Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf rust fungus)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4RRX9|F4RRX9_MELLP Uncharacterized protein OS=Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) OX=747676 GN=MELLADRAFT_72285 PE=4 SV=1
MM1 pKa = 8.0 PNGQKK6 pKa = 10.15 QLIPNAGQVAFLHH19 pKa = 6.63 DD20 pKa = 3.85 VWEE23 pKa = 4.63 CLLPTQHH30 pKa = 6.69 PNEE33 pKa = 4.41 RR34 pKa = 11.84 LPDD37 pKa = 3.49 AEE39 pKa = 4.32 FTAKK43 pKa = 10.18 YY44 pKa = 9.71 LKK46 pKa = 10.49 SLSEE50 pKa = 4.27 PYY52 pKa = 10.29 RR53 pKa = 11.84 GTLYY57 pKa = 11.05 DD58 pKa = 3.99 LDD60 pKa = 4.24 EE61 pKa = 4.96 DD62 pKa = 5.48 ADD64 pKa = 3.99 YY65 pKa = 11.61 KK66 pKa = 11.38 DD67 pKa = 5.46 DD68 pKa = 3.94 NDD70 pKa = 4.06 EE71 pKa = 4.14 EE72 pKa = 4.73 RR73 pKa = 11.84 GSNGMCILLSATNMPTSDD91 pKa = 4.17 EE92 pKa = 4.45 SKK94 pKa = 10.21 CYY96 pKa = 10.26 CDD98 pKa = 4.31 GDD100 pKa = 3.83 VGSLYY105 pKa = 10.8 NSNDD109 pKa = 3.11 EE110 pKa = 5.07 DD111 pKa = 4.41 VSQGPADD118 pKa = 4.51 DD119 pKa = 5.32 ADD121 pKa = 3.57 MDD123 pKa = 4.0 AA124 pKa = 5.5
Molecular weight: 13.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.923
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|F4S4N4|F4S4N4_MELLP Uncharacterized protein OS=Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) OX=747676 GN=MELLADRAFT_93289 PE=4 SV=1
MM1 pKa = 8.07 ILINTKK7 pKa = 9.64 NQTPSTIMIHH17 pKa = 6.87 PSTTTPSPTPKK28 pKa = 10.31 TMILINMKK36 pKa = 10.01 NQIPRR41 pKa = 11.84 KK42 pKa = 9.76 IIIHH46 pKa = 6.88 LNTTTPSPRR55 pKa = 11.84 PMTPTNTKK63 pKa = 9.16 NQTPRR68 pKa = 11.84 TIMIHH73 pKa = 6.71 PSTTTPSPRR82 pKa = 11.84 TTILTNTKK90 pKa = 9.13 NQTPIMIHH98 pKa = 6.8 LNTTTPSPRR107 pKa = 11.84 TIMILISIQNQTPRR121 pKa = 11.84 KK122 pKa = 9.83 IMIHH126 pKa = 6.71 LNTTTPSPPPRR137 pKa = 11.84 TIMIHH142 pKa = 6.76 LNTTTSSPRR151 pKa = 11.84 PMIPTSMKK159 pKa = 10.01 NQTPRR164 pKa = 11.84 NIMIHH169 pKa = 6.81 LNTTTPSPRR178 pKa = 11.84 PMIPTSIKK186 pKa = 10.15 NQTPRR191 pKa = 11.84 NIMIHH196 pKa = 6.81 LNTTTPSQSPRR207 pKa = 11.84 TIMTLISIKK216 pKa = 10.57 NQTPRR221 pKa = 11.84 KK222 pKa = 7.89 ITIHH226 pKa = 6.95 PSTTTPTPTPRR237 pKa = 11.84 TIMILINMTNLIPRR251 pKa = 11.84 KK252 pKa = 9.81 IMIHH256 pKa = 7.22 INMKK260 pKa = 10.01 NQTEE264 pKa = 4.09
Molecular weight: 29.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.082
IPC_protein 12.647
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.822
Wikipedia 13.305
Rodwell 12.618
Grimsley 12.866
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.325
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16237
0
16237
6087715
34
5272
374.9
41.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.488 ± 0.019
1.4 ± 0.007
5.504 ± 0.012
6.055 ± 0.019
3.694 ± 0.013
5.712 ± 0.021
2.796 ± 0.012
5.582 ± 0.015
5.86 ± 0.021
9.192 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.1 ± 0.008
4.722 ± 0.015
6.084 ± 0.022
4.444 ± 0.015
5.393 ± 0.017
9.581 ± 0.028
6.194 ± 0.014
5.375 ± 0.015
1.273 ± 0.007
2.552 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here