Streptobacillus moniliformis (strain ATCC 14647 / DSM 12112 / NCTC 10651 / 9901)
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1431 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1AWA7|D1AWA7_STRM9 Uncharacterized protein OS=Streptobacillus moniliformis (strain ATCC 14647 / DSM 12112 / NCTC 10651 / 9901) OX=519441 GN=Smon_0086 PE=4 SV=1
MM1 pKa = 7.09 KK2 pKa = 9.86 TGIYY6 pKa = 10.25 FGTTTGTTEE15 pKa = 5.16 DD16 pKa = 3.09 IANRR20 pKa = 11.84 ISNHH24 pKa = 5.9 FDD26 pKa = 3.15 DD27 pKa = 4.3 VDD29 pKa = 4.11 VIEE32 pKa = 4.26 VSEE35 pKa = 5.14 GIDD38 pKa = 3.18 TFSNYY43 pKa = 10.64 DD44 pKa = 3.87 LLILGSPTWGLGDD57 pKa = 5.18 LQDD60 pKa = 4.57 DD61 pKa = 3.94 WMACVDD67 pKa = 5.03 EE68 pKa = 6.12 IDD70 pKa = 6.17 DD71 pKa = 4.16 MDD73 pKa = 5.88 LSDD76 pKa = 5.19 KK77 pKa = 10.85 YY78 pKa = 11.23 VALFGTGDD86 pKa = 3.32 QASFADD92 pKa = 3.83 SFIDD96 pKa = 5.22 AIEE99 pKa = 3.97 ILYY102 pKa = 10.54 KK103 pKa = 10.51 KK104 pKa = 10.18 ALKK107 pKa = 10.62 ANAKK111 pKa = 10.09 IIGFTDD117 pKa = 2.81 IDD119 pKa = 4.44 GYY121 pKa = 11.62 DD122 pKa = 3.62 FTDD125 pKa = 3.37 SLAVKK130 pKa = 10.07 NGRR133 pKa = 11.84 FICLAIDD140 pKa = 3.96 EE141 pKa = 4.99 LNQPEE146 pKa = 4.5 LSDD149 pKa = 3.49 EE150 pKa = 5.43 RR151 pKa = 11.84 IEE153 pKa = 4.1 NWCNQLKK160 pKa = 10.66 EE161 pKa = 4.48 EE162 pKa = 4.35 IEE164 pKa = 4.24 KK165 pKa = 10.99
Molecular weight: 18.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.137
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 3.414
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|D1AWC1|D1AWC1_STRM9 Type II site-specific deoxyribonuclease OS=Streptobacillus moniliformis (strain ATCC 14647 / DSM 12112 / NCTC 10651 / 9901) OX=519441 GN=Smon_0101 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.21 RR10 pKa = 11.84 KK11 pKa = 10.24 RR12 pKa = 11.84 KK13 pKa = 7.34 MDD15 pKa = 3.12 HH16 pKa = 6.11 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.2 SGRR28 pKa = 11.84 NVLKK32 pKa = 10.45 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.53 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 10.833
IPC_protein 12.223
Toseland 12.384
ProMoST 12.866
Dawson 12.398
Bjellqvist 12.384
Wikipedia 12.852
Rodwell 12.223
Grimsley 12.427
Solomon 12.881
Lehninger 12.778
Nozaki 12.384
DTASelect 12.384
Thurlkill 12.384
EMBOSS 12.881
Sillero 12.384
Patrickios 11.959
IPC_peptide 12.881
IPC2_peptide 11.857
IPC2.peptide.svr19 9.014
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1431
0
1431
484340
33
2192
338.5
38.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.734 ± 0.075
0.533 ± 0.019
5.609 ± 0.057
7.817 ± 0.076
4.72 ± 0.064
6.15 ± 0.082
1.276 ± 0.021
10.404 ± 0.088
10.365 ± 0.081
9.475 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.03
7.301 ± 0.081
2.23 ± 0.035
1.879 ± 0.027
3.237 ± 0.039
6.437 ± 0.058
4.711 ± 0.056
5.794 ± 0.052
0.57 ± 0.019
4.501 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here