Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek s disease herpesvirus type 1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Mardivirus; Gallid alphaherpesvirus 2

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q77MR5|VP16_GAHVM Tegument protein VP16 homolog OS=Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) OX=10389 GN=MDV061 PE=3 SV=1
MM1 pKa = 7.58GLMDD5 pKa = 3.88IHH7 pKa = 6.54NAVCSLVIGVAILIATSQATFVDD30 pKa = 3.87WGSSITSMGDD40 pKa = 2.94FWEE43 pKa = 4.92STCSAVGVSIAFSSGFSVLFYY64 pKa = 9.71MGLVAVISALLAGSYY79 pKa = 8.93HH80 pKa = 5.92ACFRR84 pKa = 11.84LFTADD89 pKa = 3.19MFKK92 pKa = 11.09EE93 pKa = 4.32EE94 pKa = 4.02WW95 pKa = 3.25

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9E6P5|KITH_GAHVM Thymidine kinase OS=Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) OX=10389 GN=TK PE=3 SV=1
MM1 pKa = 7.53SSEE4 pKa = 4.29MPLPTTIVAPTCSMGEE20 pKa = 4.01KK21 pKa = 10.18NVLKK25 pKa = 10.33RR26 pKa = 11.84YY27 pKa = 8.91RR28 pKa = 11.84RR29 pKa = 11.84QVSSTRR35 pKa = 11.84NFKK38 pKa = 10.58RR39 pKa = 11.84HH40 pKa = 4.74VNTNVLRR47 pKa = 11.84KK48 pKa = 9.12RR49 pKa = 11.84RR50 pKa = 11.84LAAGVRR56 pKa = 11.84CHH58 pKa = 6.91DD59 pKa = 3.41RR60 pKa = 11.84FYY62 pKa = 11.5KK63 pKa = 10.3RR64 pKa = 11.84LYY66 pKa = 10.22AEE68 pKa = 4.41AMCLGSQVYY77 pKa = 9.4DD78 pKa = 3.2WPGRR82 pKa = 11.84SFAKK86 pKa = 10.31IFGKK90 pKa = 10.93VMVLDD95 pKa = 3.81VFKK98 pKa = 11.06QLTDD102 pKa = 3.64FRR104 pKa = 11.84LVFEE108 pKa = 4.72VNLEE112 pKa = 3.88RR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84PDD117 pKa = 4.09CICMFKK123 pKa = 10.72LPRR126 pKa = 11.84DD127 pKa = 3.6LWEE130 pKa = 4.18VGCDD134 pKa = 3.72GVCVILEE141 pKa = 4.17LKK143 pKa = 8.3TCKK146 pKa = 10.03FSRR149 pKa = 11.84NLKK152 pKa = 8.43TRR154 pKa = 11.84SKK156 pKa = 10.7HH157 pKa = 4.12EE158 pKa = 3.8QRR160 pKa = 11.84LTGIKK165 pKa = 9.8QLLDD169 pKa = 3.61SKK171 pKa = 11.32SLIGQIAPQGSDD183 pKa = 4.05CIVICPMLVFVARR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 10.36LSVLHH203 pKa = 6.09VMCLKK208 pKa = 10.19KK209 pKa = 10.54RR210 pKa = 11.84HH211 pKa = 5.93IVTDD215 pKa = 4.1FHH217 pKa = 6.89RR218 pKa = 11.84LCSILATASDD228 pKa = 3.98YY229 pKa = 10.86KK230 pKa = 11.33ARR232 pKa = 11.84ITDD235 pKa = 3.07THH237 pKa = 6.84KK238 pKa = 10.25ILKK241 pKa = 9.44RR242 pKa = 11.84HH243 pKa = 5.08NTNTIHH249 pKa = 6.07IQRR252 pKa = 11.84RR253 pKa = 11.84SGKK256 pKa = 10.14ASANVTDD263 pKa = 4.46LRR265 pKa = 11.84NHH267 pKa = 6.44IACNKK272 pKa = 7.38TATLSFSNNQEE283 pKa = 4.14RR284 pKa = 11.84TGGTAMRR291 pKa = 11.84NMANIIARR299 pKa = 11.84LVQRR303 pKa = 11.84PP304 pKa = 3.28

Molecular weight: 34.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

1

91

42836

84

3342

470.7

52.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.169 ± 0.187

2.386 ± 0.136

5.507 ± 0.167

5.355 ± 0.153

3.803 ± 0.176

5.619 ± 0.174

2.493 ± 0.08

6.546 ± 0.245

4.249 ± 0.196

9.205 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.126

4.337 ± 0.156

5.57 ± 0.506

2.685 ± 0.1

6.665 ± 0.253

8.635 ± 0.285

6.695 ± 0.143

5.843 ± 0.14

1.137 ± 0.073

3.497 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski