Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Average proteome isoelectric point is 7.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 628 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6KHK8|Q6KHK8_MYCMO Expressed protein OS=Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711) OX=267748 GN=MMOB4360 PE=4 SV=1
MM1 pKa = 7.23 MKK3 pKa = 10.03 INNEE7 pKa = 3.78 IKK9 pKa = 10.11 DD10 pKa = 3.67 LRR12 pKa = 11.84 EE13 pKa = 3.35 IDD15 pKa = 4.12 VYY17 pKa = 11.5 DD18 pKa = 3.88 EE19 pKa = 4.2 NGKK22 pKa = 9.34 KK23 pKa = 10.5 LKK25 pKa = 10.86 GIVLFTFEE33 pKa = 4.36 EE34 pKa = 4.45 AGDD37 pKa = 3.73 TFICYY42 pKa = 8.63 TIKK45 pKa = 11.01 EE46 pKa = 4.28 EE47 pKa = 4.76 VFFSKK52 pKa = 10.8 LNEE55 pKa = 4.59 DD56 pKa = 3.56 NSLSNLEE63 pKa = 3.78 EE64 pKa = 4.37 DD65 pKa = 3.66 EE66 pKa = 4.45 YY67 pKa = 11.59 KK68 pKa = 10.3 IVEE71 pKa = 4.42 QIWEE75 pKa = 4.47 DD76 pKa = 4.37 FIQSDD81 pKa = 3.76 KK82 pKa = 11.1 FKK84 pKa = 11.11 EE85 pKa = 3.89 LAEE88 pKa = 4.37 KK89 pKa = 11.24 YY90 pKa = 9.62 NFEE93 pKa = 4.85 GIGGPLVDD101 pKa = 5.52 GGDD104 pKa = 4.67 DD105 pKa = 4.28 EE106 pKa = 7.74 DD107 pKa = 6.87 DD108 pKa = 4.57 DD109 pKa = 7.09 DD110 pKa = 5.76 EE111 pKa = 7.53 DD112 pKa = 5.73 EE113 pKa = 5.92 DD114 pKa = 6.33 DD115 pKa = 5.86 EE116 pKa = 7.09 DD117 pKa = 6.6 DD118 pKa = 4.61 EE119 pKa = 7.19 DD120 pKa = 5.98 EE121 pKa = 5.42 DD122 pKa = 6.5 DD123 pKa = 4.7 EE124 pKa = 7.25 DD125 pKa = 4.7 EE126 pKa = 6.07 DD127 pKa = 6.13 DD128 pKa = 4.62 DD129 pKa = 4.15 EE130 pKa = 5.42 EE131 pKa = 5.46 YY132 pKa = 11.31 YY133 pKa = 11.36 GDD135 pKa = 3.74 YY136 pKa = 11.34 DD137 pKa = 3.88 EE138 pKa = 7.07 DD139 pKa = 3.86 EE140 pKa = 5.09 DD141 pKa = 5.12 EE142 pKa = 4.57 YY143 pKa = 11.61 EE144 pKa = 3.81 EE145 pKa = 5.61 DD146 pKa = 3.73 YY147 pKa = 11.83 EE148 pKa = 4.87 EE149 pKa = 4.12 FSKK152 pKa = 10.86 DD153 pKa = 3.49 IKK155 pKa = 10.92 DD156 pKa = 3.46 SYY158 pKa = 11.31 KK159 pKa = 11.02 NLIEE163 pKa = 4.3 KK164 pKa = 10.57 RR165 pKa = 11.84 NIINN169 pKa = 3.4
Molecular weight: 20.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.17
IPC2_protein 3.617
IPC_protein 3.63
Toseland 3.414
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.528
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.554
Nozaki 3.719
DTASelect 3.923
Thurlkill 3.452
EMBOSS 3.541
Sillero 3.745
Patrickios 3.249
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|Q6KHR1|Q6KHR1_MYCMO Expressed protein OS=Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711) OX=267748 GN=MMOB3810 PE=4 SV=1
MM1 pKa = 8.05 LYY3 pKa = 10.22 RR4 pKa = 11.84 LSYY7 pKa = 7.9 RR8 pKa = 11.84 AKK10 pKa = 7.72 WRR12 pKa = 11.84 SRR14 pKa = 11.84 RR15 pKa = 11.84 GSNPRR20 pKa = 11.84 SPPWQGGVLNHH31 pKa = 5.94 FTTEE35 pKa = 3.93 PLVAEE40 pKa = 4.46 VGIEE44 pKa = 3.82 PTTFRR49 pKa = 11.84 LWAWRR54 pKa = 11.84 ATTALLRR61 pKa = 11.84 DD62 pKa = 4.01 IIFLCKK68 pKa = 10.41 FIIAKK73 pKa = 10.0 IKK75 pKa = 9.93 IFVNFFEE82 pKa = 5.33 IYY84 pKa = 10.07 LNKK87 pKa = 10.3 KK88 pKa = 8.21 IQQILNFLWWYY99 pKa = 9.04 NKK101 pKa = 7.5 WRR103 pKa = 11.84 AMRR106 pKa = 11.84 DD107 pKa = 3.3 SNPRR111 pKa = 11.84 EE112 pKa = 4.07 FYY114 pKa = 11.06 LLTVFKK120 pKa = 9.64 TVPFSQTWVIALFGSPSWDD139 pKa = 2.97 WTSDD143 pKa = 3.09 LTIISRR149 pKa = 11.84 VLL151 pKa = 3.08
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.809
IPC_protein 10.54
Toseland 10.643
ProMoST 10.321
Dawson 10.76
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 11.052
Grimsley 10.818
Solomon 10.847
Lehninger 10.818
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.687
Patrickios 10.774
IPC_peptide 10.847
IPC2_peptide 9.341
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
628
0
628
232639
38
4727
370.4
42.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.807 ± 0.099
0.268 ± 0.016
4.696 ± 0.067
6.933 ± 0.13
6.323 ± 0.103
4.809 ± 0.097
1.253 ± 0.038
10.634 ± 0.102
10.001 ± 0.253
9.666 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.511 ± 0.042
8.492 ± 0.177
2.646 ± 0.067
3.014 ± 0.064
2.879 ± 0.064
7.488 ± 0.114
5.159 ± 0.124
5.243 ± 0.069
0.81 ± 0.034
3.367 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here