Salinarchaeum sp. Harcht-Bsk1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Salinarchaeum; unclassified Salinarchaeum

Average proteome isoelectric point is 4.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R4VYG8|R4VYG8_9EURY Peptide methionine sulfoxide reductase MsrB OS=Salinarchaeum sp. Harcht-Bsk1 OX=1333523 GN=msrB PE=3 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.66DD4 pKa = 4.18DD5 pKa = 4.33TTIEE9 pKa = 3.98LSRR12 pKa = 11.84RR13 pKa = 11.84TVLGGLGGIGVAATGAALGTSAYY36 pKa = 8.83FTDD39 pKa = 5.09DD40 pKa = 3.06EE41 pKa = 4.6AFEE44 pKa = 4.71GNSLAAGSLDD54 pKa = 5.25LKK56 pKa = 11.03VDD58 pKa = 3.07WQEE61 pKa = 3.8WYY63 pKa = 10.87NGTKK67 pKa = 9.62IEE69 pKa = 5.28AYY71 pKa = 9.21PDD73 pKa = 4.0PDD75 pKa = 3.47NDD77 pKa = 3.8GVQDD81 pKa = 4.06AFASEE86 pKa = 4.45PGQTTAKK93 pKa = 10.12GVGYY97 pKa = 10.2VCEE100 pKa = 5.34DD101 pKa = 3.83GADD104 pKa = 3.77TPADD108 pKa = 4.13LDD110 pKa = 4.14PEE112 pKa = 4.5HH113 pKa = 7.0SLRR116 pKa = 11.84TTVSGEE122 pKa = 4.25TFGSGPPLPLVHH134 pKa = 6.66LTDD137 pKa = 5.42LKK139 pKa = 11.15PGDD142 pKa = 4.39CGGLTLSFHH151 pKa = 6.87LCDD154 pKa = 3.42NPGFVWMNGTLTEE167 pKa = 4.02NAEE170 pKa = 4.14NDD172 pKa = 3.43VTEE175 pKa = 4.74PEE177 pKa = 4.4SAVDD181 pKa = 4.22DD182 pKa = 3.92SDD184 pKa = 4.52GQGEE188 pKa = 4.18LADD191 pKa = 5.1AIRR194 pKa = 11.84TRR196 pKa = 11.84LWYY199 pKa = 10.0DD200 pKa = 3.53ADD202 pKa = 4.26CDD204 pKa = 4.04GEE206 pKa = 4.57RR207 pKa = 11.84DD208 pKa = 3.88DD209 pKa = 5.71GEE211 pKa = 4.52VPITPEE217 pKa = 3.63LSLAEE222 pKa = 3.98LLGVLGSDD230 pKa = 4.13HH231 pKa = 7.51GIPLDD236 pKa = 4.13GALGDD241 pKa = 4.64DD242 pKa = 4.32GATIAQAPSLGPGTCPEE259 pKa = 4.09TPYY262 pKa = 11.47DD263 pKa = 3.78EE264 pKa = 4.49VTTGTEE270 pKa = 3.88EE271 pKa = 4.32FPNGTTRR278 pKa = 11.84QRR280 pKa = 11.84MNPKK284 pKa = 10.0CSDD287 pKa = 3.44FGLVEE292 pKa = 4.12AVRR295 pKa = 11.84IGSDD299 pKa = 3.51EE300 pKa = 4.34PGGPLPAPGACATYY314 pKa = 8.42EE315 pKa = 3.88TDD317 pKa = 3.47YY318 pKa = 11.71GDD320 pKa = 4.07VRR322 pKa = 11.84VCRR325 pKa = 11.84DD326 pKa = 3.08ADD328 pKa = 3.64GTITSWEE335 pKa = 4.24TDD337 pKa = 3.52DD338 pKa = 5.87SPDD341 pKa = 3.41GSFDD345 pKa = 3.38ASGDD349 pKa = 3.75GFCVSKK355 pKa = 11.11VVVKK359 pKa = 10.76GGNQGANIYY368 pKa = 10.48HH369 pKa = 6.98YY370 pKa = 10.92DD371 pKa = 3.57RR372 pKa = 11.84DD373 pKa = 3.81ADD375 pKa = 3.76PVEE378 pKa = 4.5RR379 pKa = 11.84SSGDD383 pKa = 3.01EE384 pKa = 4.06GSFVTPTGQDD394 pKa = 3.37YY395 pKa = 11.36SHH397 pKa = 7.22VSVCVDD403 pKa = 3.99LVAEE407 pKa = 5.2DD408 pKa = 4.46GNGDD412 pKa = 3.72DD413 pKa = 6.11GRR415 pKa = 11.84DD416 pKa = 3.48CFEE419 pKa = 4.91NSTTHH424 pKa = 6.68CVGLEE429 pKa = 3.2WWLPVDD435 pKa = 5.2RR436 pKa = 11.84GNEE439 pKa = 3.92LQTDD443 pKa = 3.9SVGFDD448 pKa = 2.76VGFYY452 pKa = 10.99AEE454 pKa = 4.14QCRR457 pKa = 11.84HH458 pKa = 6.11NDD460 pKa = 4.74DD461 pKa = 4.09PPQTQRR467 pKa = 11.84IEE469 pKa = 4.12LGKK472 pKa = 10.37DD473 pKa = 2.76AARR476 pKa = 11.84NTGFEE481 pKa = 4.14AVSDD485 pKa = 3.62GGYY488 pKa = 10.23FGSGYY493 pKa = 10.09WKK495 pKa = 10.27DD496 pKa = 5.44DD497 pKa = 3.52PTQTNQDD504 pKa = 3.19GGTYY508 pKa = 8.57EE509 pKa = 3.97QLYY512 pKa = 8.4LTFDD516 pKa = 3.45QSFGPYY522 pKa = 8.74YY523 pKa = 10.81VSALAPFTIGDD534 pKa = 3.79IQSIRR539 pKa = 11.84YY540 pKa = 6.81RR541 pKa = 11.84TNTPSGVSQNYY552 pKa = 8.88FLEE555 pKa = 5.46IYY557 pKa = 8.9TSPDD561 pKa = 2.78GSNDD565 pKa = 3.26DD566 pKa = 3.74ASWYY570 pKa = 10.38GRR572 pKa = 11.84LLQALPGDD580 pKa = 3.87ALNRR584 pKa = 11.84TVSPDD589 pKa = 2.59TWVTWRR595 pKa = 11.84TEE597 pKa = 3.71SGTNQLTFYY606 pKa = 10.76DD607 pKa = 4.46HH608 pKa = 6.57NHH610 pKa = 7.35DD611 pKa = 4.1YY612 pKa = 9.55DD613 pKa = 4.26TTDD616 pKa = 3.38STPGDD621 pKa = 3.77APNAYY626 pKa = 9.77LGQDD630 pKa = 3.43TGVTLADD637 pKa = 4.2LQSTDD642 pKa = 3.14AFDD645 pKa = 3.27WSNYY649 pKa = 9.0VSDD652 pKa = 5.28ADD654 pKa = 4.02STPKK658 pKa = 10.42NYY660 pKa = 9.55RR661 pKa = 11.84DD662 pKa = 3.48EE663 pKa = 4.2EE664 pKa = 4.2VRR666 pKa = 11.84ALRR669 pKa = 11.84FATGSAWEE677 pKa = 4.95DD678 pKa = 3.38DD679 pKa = 4.34HH680 pKa = 7.25EE681 pKa = 5.31GCLDD685 pKa = 4.27AIEE688 pKa = 4.42ITLEE692 pKa = 4.17DD693 pKa = 3.46GRR695 pKa = 11.84SAVVDD700 pKa = 4.2LEE702 pKa = 4.24PP703 pKa = 5.71

Molecular weight:
75.41 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R4W6K2|R4W6K2_9EURY UDP-glucose 4-epimerase OS=Salinarchaeum sp. Harcht-Bsk1 OX=1333523 GN=L593_02460 PE=4 SV=1
MM1 pKa = 7.44LFYY4 pKa = 11.02YY5 pKa = 9.99EE6 pKa = 4.82SQSQPISLKK15 pKa = 10.38KK16 pKa = 10.35DD17 pKa = 3.11DD18 pKa = 4.15FAQIVLMRR26 pKa = 11.84CGRR29 pKa = 11.84QSLVMEE35 pKa = 4.41VEE37 pKa = 3.97RR38 pKa = 11.84RR39 pKa = 11.84PLRR42 pKa = 11.84TSLTYY47 pKa = 11.01SLDD50 pKa = 3.31VRR52 pKa = 11.84CNTRR56 pKa = 11.84VFKK59 pKa = 10.85RR60 pKa = 11.84RR61 pKa = 11.84FSVPFIKK68 pKa = 10.29QRR70 pKa = 11.84IKK72 pKa = 9.71MVKK75 pKa = 9.93RR76 pKa = 11.84NN77 pKa = 3.37

Molecular weight:
9.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3013

0

3013

911803

31

2381

302.6

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.658 ± 0.067

0.686 ± 0.014

8.843 ± 0.052

8.881 ± 0.062

3.111 ± 0.029

8.833 ± 0.04

2.048 ± 0.021

4.013 ± 0.03

1.479 ± 0.024

8.794 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.637 ± 0.021

2.079 ± 0.027

4.815 ± 0.029

2.577 ± 0.027

6.355 ± 0.044

5.398 ± 0.041

6.154 ± 0.042

8.989 ± 0.043

1.14 ± 0.018

2.511 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski