Salinarchaeum sp. Harcht-Bsk1
Average proteome isoelectric point is 4.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4VYG8|R4VYG8_9EURY Peptide methionine sulfoxide reductase MsrB OS=Salinarchaeum sp. Harcht-Bsk1 OX=1333523 GN=msrB PE=3 SV=1
MM1 pKa = 7.74 TDD3 pKa = 3.66 DD4 pKa = 4.18 DD5 pKa = 4.33 TTIEE9 pKa = 3.98 LSRR12 pKa = 11.84 RR13 pKa = 11.84 TVLGGLGGIGVAATGAALGTSAYY36 pKa = 8.83 FTDD39 pKa = 5.09 DD40 pKa = 3.06 EE41 pKa = 4.6 AFEE44 pKa = 4.71 GNSLAAGSLDD54 pKa = 5.25 LKK56 pKa = 11.03 VDD58 pKa = 3.07 WQEE61 pKa = 3.8 WYY63 pKa = 10.87 NGTKK67 pKa = 9.62 IEE69 pKa = 5.28 AYY71 pKa = 9.21 PDD73 pKa = 4.0 PDD75 pKa = 3.47 NDD77 pKa = 3.8 GVQDD81 pKa = 4.06 AFASEE86 pKa = 4.45 PGQTTAKK93 pKa = 10.12 GVGYY97 pKa = 10.2 VCEE100 pKa = 5.34 DD101 pKa = 3.83 GADD104 pKa = 3.77 TPADD108 pKa = 4.13 LDD110 pKa = 4.14 PEE112 pKa = 4.5 HH113 pKa = 7.0 SLRR116 pKa = 11.84 TTVSGEE122 pKa = 4.25 TFGSGPPLPLVHH134 pKa = 6.66 LTDD137 pKa = 5.42 LKK139 pKa = 11.15 PGDD142 pKa = 4.39 CGGLTLSFHH151 pKa = 6.87 LCDD154 pKa = 3.42 NPGFVWMNGTLTEE167 pKa = 4.02 NAEE170 pKa = 4.14 NDD172 pKa = 3.43 VTEE175 pKa = 4.74 PEE177 pKa = 4.4 SAVDD181 pKa = 4.22 DD182 pKa = 3.92 SDD184 pKa = 4.52 GQGEE188 pKa = 4.18 LADD191 pKa = 5.1 AIRR194 pKa = 11.84 TRR196 pKa = 11.84 LWYY199 pKa = 10.0 DD200 pKa = 3.53 ADD202 pKa = 4.26 CDD204 pKa = 4.04 GEE206 pKa = 4.57 RR207 pKa = 11.84 DD208 pKa = 3.88 DD209 pKa = 5.71 GEE211 pKa = 4.52 VPITPEE217 pKa = 3.63 LSLAEE222 pKa = 3.98 LLGVLGSDD230 pKa = 4.13 HH231 pKa = 7.51 GIPLDD236 pKa = 4.13 GALGDD241 pKa = 4.64 DD242 pKa = 4.32 GATIAQAPSLGPGTCPEE259 pKa = 4.09 TPYY262 pKa = 11.47 DD263 pKa = 3.78 EE264 pKa = 4.49 VTTGTEE270 pKa = 3.88 EE271 pKa = 4.32 FPNGTTRR278 pKa = 11.84 QRR280 pKa = 11.84 MNPKK284 pKa = 10.0 CSDD287 pKa = 3.44 FGLVEE292 pKa = 4.12 AVRR295 pKa = 11.84 IGSDD299 pKa = 3.51 EE300 pKa = 4.34 PGGPLPAPGACATYY314 pKa = 8.42 EE315 pKa = 3.88 TDD317 pKa = 3.47 YY318 pKa = 11.71 GDD320 pKa = 4.07 VRR322 pKa = 11.84 VCRR325 pKa = 11.84 DD326 pKa = 3.08 ADD328 pKa = 3.64 GTITSWEE335 pKa = 4.24 TDD337 pKa = 3.52 DD338 pKa = 5.87 SPDD341 pKa = 3.41 GSFDD345 pKa = 3.38 ASGDD349 pKa = 3.75 GFCVSKK355 pKa = 11.11 VVVKK359 pKa = 10.76 GGNQGANIYY368 pKa = 10.48 HH369 pKa = 6.98 YY370 pKa = 10.92 DD371 pKa = 3.57 RR372 pKa = 11.84 DD373 pKa = 3.81 ADD375 pKa = 3.76 PVEE378 pKa = 4.5 RR379 pKa = 11.84 SSGDD383 pKa = 3.01 EE384 pKa = 4.06 GSFVTPTGQDD394 pKa = 3.37 YY395 pKa = 11.36 SHH397 pKa = 7.22 VSVCVDD403 pKa = 3.99 LVAEE407 pKa = 5.2 DD408 pKa = 4.46 GNGDD412 pKa = 3.72 DD413 pKa = 6.11 GRR415 pKa = 11.84 DD416 pKa = 3.48 CFEE419 pKa = 4.91 NSTTHH424 pKa = 6.68 CVGLEE429 pKa = 3.2 WWLPVDD435 pKa = 5.2 RR436 pKa = 11.84 GNEE439 pKa = 3.92 LQTDD443 pKa = 3.9 SVGFDD448 pKa = 2.76 VGFYY452 pKa = 10.99 AEE454 pKa = 4.14 QCRR457 pKa = 11.84 HH458 pKa = 6.11 NDD460 pKa = 4.74 DD461 pKa = 4.09 PPQTQRR467 pKa = 11.84 IEE469 pKa = 4.12 LGKK472 pKa = 10.37 DD473 pKa = 2.76 AARR476 pKa = 11.84 NTGFEE481 pKa = 4.14 AVSDD485 pKa = 3.62 GGYY488 pKa = 10.23 FGSGYY493 pKa = 10.09 WKK495 pKa = 10.27 DD496 pKa = 5.44 DD497 pKa = 3.52 PTQTNQDD504 pKa = 3.19 GGTYY508 pKa = 8.57 EE509 pKa = 3.97 QLYY512 pKa = 8.4 LTFDD516 pKa = 3.45 QSFGPYY522 pKa = 8.74 YY523 pKa = 10.81 VSALAPFTIGDD534 pKa = 3.79 IQSIRR539 pKa = 11.84 YY540 pKa = 6.81 RR541 pKa = 11.84 TNTPSGVSQNYY552 pKa = 8.88 FLEE555 pKa = 5.46 IYY557 pKa = 8.9 TSPDD561 pKa = 2.78 GSNDD565 pKa = 3.26 DD566 pKa = 3.74 ASWYY570 pKa = 10.38 GRR572 pKa = 11.84 LLQALPGDD580 pKa = 3.87 ALNRR584 pKa = 11.84 TVSPDD589 pKa = 2.59 TWVTWRR595 pKa = 11.84 TEE597 pKa = 3.71 SGTNQLTFYY606 pKa = 10.76 DD607 pKa = 4.46 HH608 pKa = 6.57 NHH610 pKa = 7.35 DD611 pKa = 4.1 YY612 pKa = 9.55 DD613 pKa = 4.26 TTDD616 pKa = 3.38 STPGDD621 pKa = 3.77 APNAYY626 pKa = 9.77 LGQDD630 pKa = 3.43 TGVTLADD637 pKa = 4.2 LQSTDD642 pKa = 3.14 AFDD645 pKa = 3.27 WSNYY649 pKa = 9.0 VSDD652 pKa = 5.28 ADD654 pKa = 4.02 STPKK658 pKa = 10.42 NYY660 pKa = 9.55 RR661 pKa = 11.84 DD662 pKa = 3.48 EE663 pKa = 4.2 EE664 pKa = 4.2 VRR666 pKa = 11.84 ALRR669 pKa = 11.84 FATGSAWEE677 pKa = 4.95 DD678 pKa = 3.38 DD679 pKa = 4.34 HH680 pKa = 7.25 EE681 pKa = 5.31 GCLDD685 pKa = 4.27 AIEE688 pKa = 4.42 ITLEE692 pKa = 4.17 DD693 pKa = 3.46 GRR695 pKa = 11.84 SAVVDD700 pKa = 4.2 LEE702 pKa = 4.24 PP703 pKa = 5.71
Molecular weight: 75.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.897
Patrickios 1.189
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|R4W6K2|R4W6K2_9EURY UDP-glucose 4-epimerase OS=Salinarchaeum sp. Harcht-Bsk1 OX=1333523 GN=L593_02460 PE=4 SV=1
MM1 pKa = 7.44 LFYY4 pKa = 11.02 YY5 pKa = 9.99 EE6 pKa = 4.82 SQSQPISLKK15 pKa = 10.38 KK16 pKa = 10.35 DD17 pKa = 3.11 DD18 pKa = 4.15 FAQIVLMRR26 pKa = 11.84 CGRR29 pKa = 11.84 QSLVMEE35 pKa = 4.41 VEE37 pKa = 3.97 RR38 pKa = 11.84 RR39 pKa = 11.84 PLRR42 pKa = 11.84 TSLTYY47 pKa = 11.01 SLDD50 pKa = 3.31 VRR52 pKa = 11.84 CNTRR56 pKa = 11.84 VFKK59 pKa = 10.85 RR60 pKa = 11.84 RR61 pKa = 11.84 FSVPFIKK68 pKa = 10.29 QRR70 pKa = 11.84 IKK72 pKa = 9.71 MVKK75 pKa = 9.93 RR76 pKa = 11.84 NN77 pKa = 3.37
Molecular weight: 9.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.838
IPC_protein 10.745
Toseland 10.877
ProMoST 10.657
Dawson 10.965
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.155
Grimsley 11.008
Solomon 11.125
Lehninger 11.082
Nozaki 10.862
DTASelect 10.701
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.906
Patrickios 10.906
IPC_peptide 11.125
IPC2_peptide 9.838
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3013
0
3013
911803
31
2381
302.6
32.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.658 ± 0.067
0.686 ± 0.014
8.843 ± 0.052
8.881 ± 0.062
3.111 ± 0.029
8.833 ± 0.04
2.048 ± 0.021
4.013 ± 0.03
1.479 ± 0.024
8.794 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.637 ± 0.021
2.079 ± 0.027
4.815 ± 0.029
2.577 ± 0.027
6.355 ± 0.044
5.398 ± 0.041
6.154 ± 0.042
8.989 ± 0.043
1.14 ± 0.018
2.511 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here