Gordonia phage Gaea
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3M8W0|A0A3G3M8W0_9CAUD Uncharacterized protein OS=Gordonia phage Gaea OX=2483669 GN=63 PE=4 SV=1
MM1 pKa = 7.64 SDD3 pKa = 3.12 AAGPRR8 pKa = 11.84 ADD10 pKa = 3.83 EE11 pKa = 4.85 CSCPMTDD18 pKa = 3.61 PGVPGIRR25 pKa = 11.84 PAEE28 pKa = 4.04 FEE30 pKa = 3.87 QDD32 pKa = 3.63 PLCWHH37 pKa = 6.95 HH38 pKa = 7.02 PMDD41 pKa = 4.88 GGTFLDD47 pKa = 4.63 PVQSAPIGWIQPRR60 pKa = 11.84 YY61 pKa = 9.02 YY62 pKa = 11.04 VEE64 pKa = 4.28 TFDD67 pKa = 5.44 AAPSSAWLPVADD79 pKa = 5.72 LPDD82 pKa = 4.04 LHH84 pKa = 7.11 PSDD87 pKa = 5.37 DD88 pKa = 3.85 PVIVGGDD95 pKa = 3.48 GRR97 pKa = 11.84 PWHH100 pKa = 6.69 PLPDD104 pKa = 4.1 GTWAPIEE111 pKa = 4.07 PTT113 pKa = 3.41
Molecular weight: 12.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.694
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.884
Rodwell 3.745
Grimsley 3.605
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.77
EMBOSS 3.884
Sillero 4.037
Patrickios 1.939
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A3G3M9X6|A0A3G3M9X6_9CAUD Uncharacterized protein OS=Gordonia phage Gaea OX=2483669 GN=46 PE=4 SV=1
MM1 pKa = 7.37 IPGVRR6 pKa = 11.84 FRR8 pKa = 11.84 ATRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 PSGLITFDD21 pKa = 3.18 NSTVLDD27 pKa = 4.74 GSATTSFGLTVGGSNATIIVFAAGNLTNAATVDD60 pKa = 4.05 GVAMTLIGKK69 pKa = 9.42 SSNAAMWAITGLAAGARR86 pKa = 11.84 NVVVTRR92 pKa = 11.84 AAATSYY98 pKa = 10.2 IVSAMSYY105 pKa = 10.42 LGAASFAGGVVNSGTGTAMSSAPTGGQLKK134 pKa = 10.21 VAGFDD139 pKa = 3.99 FSSSSADD146 pKa = 3.33 FAGITTDD153 pKa = 2.73 GTQRR157 pKa = 11.84 VKK159 pKa = 10.78 YY160 pKa = 10.35 RR161 pKa = 11.84 RR162 pKa = 11.84 VNGNNVLVADD172 pKa = 4.81 KK173 pKa = 8.75 PTGPIGATLVGSSSWTSIGVSLAAA197 pKa = 4.98
Molecular weight: 19.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.809
IPC_protein 10.76
Toseland 10.76
ProMoST 10.54
Dawson 10.862
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 10.994
Grimsley 10.921
Solomon 11.008
Lehninger 10.965
Nozaki 10.716
DTASelect 10.613
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.76
IPC_peptide 11.008
IPC2_peptide 9.516
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
18398
27
1670
213.9
23.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.431 ± 0.391
0.772 ± 0.116
7.669 ± 0.554
5.414 ± 0.416
2.397 ± 0.171
8.778 ± 0.498
2.06 ± 0.183
4.441 ± 0.257
2.44 ± 0.175
7.68 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.104
2.707 ± 0.152
6.18 ± 0.272
3.718 ± 0.174
7.485 ± 0.449
4.984 ± 0.294
6.533 ± 0.298
7.887 ± 0.213
2.109 ± 0.123
2.169 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here