Amino acid dipepetide frequency for Gordonia phage Gaea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.285AlaAla: 17.285 ± 1.357
0.815AlaCys: 0.815 ± 0.224
9.567AlaAsp: 9.567 ± 1.088
8.48AlaGlu: 8.48 ± 0.851
3.207AlaPhe: 3.207 ± 0.46
12.665AlaGly: 12.665 ± 1.135
2.011AlaHis: 2.011 ± 0.288
6.74AlaIle: 6.74 ± 0.947
4.131AlaLys: 4.131 ± 0.44
9.784AlaLeu: 9.784 ± 1.14
2.663AlaMet: 2.663 ± 0.449
3.207AlaAsn: 3.207 ± 0.583
6.36AlaPro: 6.36 ± 0.655
4.783AlaGln: 4.783 ± 0.468
7.882AlaArg: 7.882 ± 0.686
5.871AlaSer: 5.871 ± 0.845
6.414AlaThr: 6.414 ± 0.615
7.936AlaVal: 7.936 ± 0.588
2.283AlaTrp: 2.283 ± 0.39
2.229AlaTyr: 2.229 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
1.359CysAla: 1.359 ± 0.267
0.054CysCys: 0.054 ± 0.062
0.598CysAsp: 0.598 ± 0.199
0.435CysGlu: 0.435 ± 0.145
0.109CysPhe: 0.109 ± 0.078
1.359CysGly: 1.359 ± 0.359
0.217CysHis: 0.217 ± 0.135
0.109CysIle: 0.109 ± 0.075
0.109CysLys: 0.109 ± 0.081
0.326CysLeu: 0.326 ± 0.143
0.054CysMet: 0.054 ± 0.061
0.163CysAsn: 0.163 ± 0.089
0.489CysPro: 0.489 ± 0.166
0.326CysGln: 0.326 ± 0.156
0.544CysArg: 0.544 ± 0.197
0.652CysSer: 0.652 ± 0.205
0.38CysThr: 0.38 ± 0.14
0.38CysVal: 0.38 ± 0.139
0.054CysTrp: 0.054 ± 0.061
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.914AspAla: 8.914 ± 0.995
0.217AspCys: 0.217 ± 0.102
8.045AspAsp: 8.045 ± 1.15
6.577AspGlu: 6.577 ± 1.049
1.033AspPhe: 1.033 ± 0.312
7.338AspGly: 7.338 ± 0.671
1.576AspHis: 1.576 ± 0.331
1.848AspIle: 1.848 ± 0.254
1.141AspLys: 1.141 ± 0.217
5.49AspLeu: 5.49 ± 0.623
1.468AspMet: 1.468 ± 0.275
2.337AspAsn: 2.337 ± 0.351
7.882AspPro: 7.882 ± 0.842
2.5AspGln: 2.5 ± 0.444
4.729AspArg: 4.729 ± 0.576
2.555AspSer: 2.555 ± 0.329
3.316AspThr: 3.316 ± 0.473
6.251AspVal: 6.251 ± 0.563
1.576AspTrp: 1.576 ± 0.282
1.902AspTyr: 1.902 ± 0.423
0.0AspXaa: 0.0 ± 0.0
Glu
5.653GluAla: 5.653 ± 0.676
0.544GluCys: 0.544 ± 0.186
3.153GluAsp: 3.153 ± 0.535
1.359GluGlu: 1.359 ± 0.346
1.794GluPhe: 1.794 ± 0.337
3.696GluGly: 3.696 ± 0.503
2.066GluHis: 2.066 ± 0.414
2.663GluIle: 2.663 ± 0.309
1.305GluLys: 1.305 ± 0.281
5.11GluLeu: 5.11 ± 0.706
0.924GluMet: 0.924 ± 0.236
1.468GluAsn: 1.468 ± 0.254
3.153GluPro: 3.153 ± 0.485
3.696GluGln: 3.696 ± 0.533
3.642GluArg: 3.642 ± 0.529
2.174GluSer: 2.174 ± 0.439
3.098GluThr: 3.098 ± 0.529
4.729GluVal: 4.729 ± 0.583
1.848GluTrp: 1.848 ± 0.463
2.066GluTyr: 2.066 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
3.751PheAla: 3.751 ± 0.493
0.435PheCys: 0.435 ± 0.198
2.446PheAsp: 2.446 ± 0.379
1.631PheGlu: 1.631 ± 0.376
0.489PhePhe: 0.489 ± 0.211
2.99PheGly: 2.99 ± 0.409
0.489PheHis: 0.489 ± 0.144
1.305PheIle: 1.305 ± 0.274
0.598PheLys: 0.598 ± 0.185
1.848PheLeu: 1.848 ± 0.356
0.435PheMet: 0.435 ± 0.118
0.38PheAsn: 0.38 ± 0.16
0.924PhePro: 0.924 ± 0.201
0.435PheGln: 0.435 ± 0.138
1.087PheArg: 1.087 ± 0.202
1.033PheSer: 1.033 ± 0.223
1.576PheThr: 1.576 ± 0.283
1.576PheVal: 1.576 ± 0.322
0.326PheTrp: 0.326 ± 0.137
0.217PheTyr: 0.217 ± 0.102
0.0PheXaa: 0.0 ± 0.0
Gly
9.947GlyAla: 9.947 ± 1.216
0.707GlyCys: 0.707 ± 0.21
6.088GlyAsp: 6.088 ± 0.773
4.131GlyGlu: 4.131 ± 0.484
2.066GlyPhe: 2.066 ± 0.397
8.099GlyGly: 8.099 ± 1.142
2.174GlyHis: 2.174 ± 0.414
3.751GlyIle: 3.751 ± 0.467
2.935GlyLys: 2.935 ± 0.537
6.034GlyLeu: 6.034 ± 0.966
2.609GlyMet: 2.609 ± 0.368
2.283GlyAsn: 2.283 ± 0.393
3.968GlyPro: 3.968 ± 0.563
3.207GlyGln: 3.207 ± 0.502
7.556GlyArg: 7.556 ± 0.734
5.327GlySer: 5.327 ± 0.576
6.197GlyThr: 6.197 ± 0.761
7.121GlyVal: 7.121 ± 0.721
1.413GlyTrp: 1.413 ± 0.301
2.174GlyTyr: 2.174 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.902HisAla: 1.902 ± 0.295
0.0HisCys: 0.0 ± 0.0
1.739HisAsp: 1.739 ± 0.301
1.685HisGlu: 1.685 ± 0.362
0.326HisPhe: 0.326 ± 0.126
1.25HisGly: 1.25 ± 0.274
0.38HisHis: 0.38 ± 0.172
0.924HisIle: 0.924 ± 0.229
0.38HisLys: 0.38 ± 0.133
2.066HisLeu: 2.066 ± 0.403
0.707HisMet: 0.707 ± 0.201
0.272HisAsn: 0.272 ± 0.13
1.305HisPro: 1.305 ± 0.287
0.761HisGln: 0.761 ± 0.19
1.902HisArg: 1.902 ± 0.354
0.707HisSer: 0.707 ± 0.229
1.957HisThr: 1.957 ± 0.376
1.576HisVal: 1.576 ± 0.334
0.489HisTrp: 0.489 ± 0.18
0.272HisTyr: 0.272 ± 0.109
0.0HisXaa: 0.0 ± 0.0
Ile
6.142IleAla: 6.142 ± 0.661
0.163IleCys: 0.163 ± 0.089
3.642IleAsp: 3.642 ± 0.376
3.968IleGlu: 3.968 ± 0.534
0.435IlePhe: 0.435 ± 0.236
4.512IleGly: 4.512 ± 0.915
0.652IleHis: 0.652 ± 0.174
2.12IleIle: 2.12 ± 0.394
0.87IleLys: 0.87 ± 0.417
2.066IleLeu: 2.066 ± 0.311
0.652IleMet: 0.652 ± 0.199
0.707IleAsn: 0.707 ± 0.207
2.935IlePro: 2.935 ± 0.371
1.033IleGln: 1.033 ± 0.282
2.881IleArg: 2.881 ± 0.366
1.957IleSer: 1.957 ± 0.393
4.022IleThr: 4.022 ± 0.5
4.185IleVal: 4.185 ± 0.496
0.761IleTrp: 0.761 ± 0.18
0.707IleTyr: 0.707 ± 0.178
0.0IleXaa: 0.0 ± 0.0
Lys
2.772LysAla: 2.772 ± 0.435
0.054LysCys: 0.054 ± 0.059
1.087LysAsp: 1.087 ± 0.266
0.707LysGlu: 0.707 ± 0.176
1.141LysPhe: 1.141 ± 0.307
1.739LysGly: 1.739 ± 0.422
0.598LysHis: 0.598 ± 0.173
1.685LysIle: 1.685 ± 0.3
0.652LysLys: 0.652 ± 0.176
1.739LysLeu: 1.739 ± 0.29
0.598LysMet: 0.598 ± 0.161
0.707LysAsn: 0.707 ± 0.235
1.141LysPro: 1.141 ± 0.276
0.815LysGln: 0.815 ± 0.199
2.174LysArg: 2.174 ± 0.321
1.685LysSer: 1.685 ± 0.326
1.576LysThr: 1.576 ± 0.272
2.12LysVal: 2.12 ± 0.302
0.544LysTrp: 0.544 ± 0.158
0.87LysTyr: 0.87 ± 0.222
0.0LysXaa: 0.0 ± 0.0
Leu
9.567LeuAla: 9.567 ± 0.843
0.815LeuCys: 0.815 ± 0.243
5.979LeuAsp: 5.979 ± 0.798
2.555LeuGlu: 2.555 ± 0.414
2.283LeuPhe: 2.283 ± 0.393
6.795LeuGly: 6.795 ± 1.173
1.957LeuHis: 1.957 ± 0.333
3.153LeuIle: 3.153 ± 0.485
0.924LeuLys: 0.924 ± 0.234
5.762LeuLeu: 5.762 ± 0.583
1.141LeuMet: 1.141 ± 0.217
1.957LeuAsn: 1.957 ± 0.361
5.001LeuPro: 5.001 ± 0.47
3.37LeuGln: 3.37 ± 0.472
6.577LeuArg: 6.577 ± 0.465
3.316LeuSer: 3.316 ± 0.379
5.653LeuThr: 5.653 ± 0.547
6.903LeuVal: 6.903 ± 0.738
1.576LeuTrp: 1.576 ± 0.291
1.468LeuTyr: 1.468 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
1.522MetAla: 1.522 ± 0.296
0.163MetCys: 0.163 ± 0.099
0.87MetAsp: 0.87 ± 0.184
0.652MetGlu: 0.652 ± 0.208
0.544MetPhe: 0.544 ± 0.168
1.522MetGly: 1.522 ± 0.328
0.38MetHis: 0.38 ± 0.17
0.815MetIle: 0.815 ± 0.176
0.652MetLys: 0.652 ± 0.161
1.957MetLeu: 1.957 ± 0.361
0.326MetMet: 0.326 ± 0.133
0.87MetAsn: 0.87 ± 0.219
1.739MetPro: 1.739 ± 0.339
0.87MetGln: 0.87 ± 0.319
1.576MetArg: 1.576 ± 0.288
1.468MetSer: 1.468 ± 0.271
3.316MetThr: 3.316 ± 0.448
1.685MetVal: 1.685 ± 0.275
0.38MetTrp: 0.38 ± 0.143
0.163MetTyr: 0.163 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.751AsnAla: 3.751 ± 0.524
0.38AsnCys: 0.38 ± 0.139
2.229AsnAsp: 2.229 ± 0.361
1.087AsnGlu: 1.087 ± 0.299
0.544AsnPhe: 0.544 ± 0.137
3.261AsnGly: 3.261 ± 0.414
0.489AsnHis: 0.489 ± 0.162
0.978AsnIle: 0.978 ± 0.274
0.489AsnLys: 0.489 ± 0.175
2.011AsnLeu: 2.011 ± 0.35
0.761AsnMet: 0.761 ± 0.205
0.815AsnAsn: 0.815 ± 0.184
2.229AsnPro: 2.229 ± 0.399
0.435AsnGln: 0.435 ± 0.137
1.359AsnArg: 1.359 ± 0.316
1.522AsnSer: 1.522 ± 0.306
1.413AsnThr: 1.413 ± 0.263
2.011AsnVal: 2.011 ± 0.31
0.761AsnTrp: 0.761 ± 0.194
0.544AsnTyr: 0.544 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
7.882ProAla: 7.882 ± 0.711
0.326ProCys: 0.326 ± 0.192
5.979ProAsp: 5.979 ± 0.866
3.479ProGlu: 3.479 ± 0.527
1.468ProPhe: 1.468 ± 0.336
5.218ProGly: 5.218 ± 0.501
1.359ProHis: 1.359 ± 0.302
3.424ProIle: 3.424 ± 0.433
1.359ProLys: 1.359 ± 0.312
4.24ProLeu: 4.24 ± 0.488
1.196ProMet: 1.196 ± 0.294
2.337ProAsn: 2.337 ± 0.327
4.022ProPro: 4.022 ± 0.588
1.522ProGln: 1.522 ± 0.286
3.533ProArg: 3.533 ± 0.492
2.881ProSer: 2.881 ± 0.42
3.968ProThr: 3.968 ± 0.506
5.327ProVal: 5.327 ± 0.708
0.978ProTrp: 0.978 ± 0.25
1.305ProTyr: 1.305 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
5.273GlnAla: 5.273 ± 0.554
0.326GlnCys: 0.326 ± 0.106
0.978GlnAsp: 0.978 ± 0.258
1.141GlnGlu: 1.141 ± 0.206
1.087GlnPhe: 1.087 ± 0.215
1.902GlnGly: 1.902 ± 0.313
0.815GlnHis: 0.815 ± 0.291
1.631GlnIle: 1.631 ± 0.311
0.978GlnLys: 0.978 ± 0.221
3.261GlnLeu: 3.261 ± 0.398
0.924GlnMet: 0.924 ± 0.243
0.87GlnAsn: 0.87 ± 0.209
2.99GlnPro: 2.99 ± 0.414
2.881GlnGln: 2.881 ± 0.438
3.642GlnArg: 3.642 ± 0.547
1.739GlnSer: 1.739 ± 0.334
2.337GlnThr: 2.337 ± 0.407
2.935GlnVal: 2.935 ± 0.464
0.761GlnTrp: 0.761 ± 0.216
0.707GlnTyr: 0.707 ± 0.173
0.0GlnXaa: 0.0 ± 0.0
Arg
9.295ArgAla: 9.295 ± 0.722
0.544ArgCys: 0.544 ± 0.159
4.675ArgAsp: 4.675 ± 0.501
3.37ArgGlu: 3.37 ± 0.537
1.576ArgPhe: 1.576 ± 0.301
4.62ArgGly: 4.62 ± 0.724
0.978ArgHis: 0.978 ± 0.275
3.968ArgIle: 3.968 ± 0.524
2.337ArgLys: 2.337 ± 0.366
5.436ArgLeu: 5.436 ± 0.623
2.229ArgMet: 2.229 ± 0.302
2.609ArgAsn: 2.609 ± 0.394
3.642ArgPro: 3.642 ± 0.516
2.772ArgGln: 2.772 ± 0.382
9.458ArgArg: 9.458 ± 1.198
3.37ArgSer: 3.37 ± 0.409
4.022ArgThr: 4.022 ± 0.525
5.925ArgVal: 5.925 ± 0.729
2.011ArgTrp: 2.011 ± 0.442
2.011ArgTyr: 2.011 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
6.36SerAla: 6.36 ± 0.778
0.435SerCys: 0.435 ± 0.164
2.99SerAsp: 2.99 ± 0.468
2.283SerGlu: 2.283 ± 0.384
1.141SerPhe: 1.141 ± 0.241
5.653SerGly: 5.653 ± 0.642
1.196SerHis: 1.196 ± 0.24
1.902SerIle: 1.902 ± 0.322
1.413SerLys: 1.413 ± 0.366
3.588SerLeu: 3.588 ± 0.398
0.978SerMet: 0.978 ± 0.23
1.087SerAsn: 1.087 ± 0.265
2.772SerPro: 2.772 ± 0.446
1.413SerGln: 1.413 ± 0.357
3.316SerArg: 3.316 ± 0.474
3.533SerSer: 3.533 ± 0.811
3.914SerThr: 3.914 ± 0.527
3.424SerVal: 3.424 ± 0.419
1.359SerTrp: 1.359 ± 0.31
1.087SerTyr: 1.087 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
8.425ThrAla: 8.425 ± 0.773
0.652ThrCys: 0.652 ± 0.209
5.762ThrAsp: 5.762 ± 0.577
3.479ThrGlu: 3.479 ± 0.444
1.739ThrPhe: 1.739 ± 0.453
5.707ThrGly: 5.707 ± 0.64
0.87ThrHis: 0.87 ± 0.285
2.772ThrIle: 2.772 ± 0.421
1.739ThrLys: 1.739 ± 0.282
5.055ThrLeu: 5.055 ± 0.496
1.305ThrMet: 1.305 ± 0.236
1.794ThrAsn: 1.794 ± 0.319
4.838ThrPro: 4.838 ± 0.613
1.631ThrGln: 1.631 ± 0.284
3.751ThrArg: 3.751 ± 0.458
3.642ThrSer: 3.642 ± 0.498
4.294ThrThr: 4.294 ± 0.576
5.436ThrVal: 5.436 ± 0.694
1.196ThrTrp: 1.196 ± 0.248
1.25ThrTyr: 1.25 ± 0.255
0.0ThrXaa: 0.0 ± 0.0
Val
9.349ValAla: 9.349 ± 0.708
0.761ValCys: 0.761 ± 0.225
8.154ValAsp: 8.154 ± 0.72
4.512ValGlu: 4.512 ± 0.542
1.576ValPhe: 1.576 ± 0.255
6.577ValGly: 6.577 ± 0.537
1.305ValHis: 1.305 ± 0.254
3.153ValIle: 3.153 ± 0.371
1.631ValLys: 1.631 ± 0.199
6.197ValLeu: 6.197 ± 0.576
1.196ValMet: 1.196 ± 0.263
2.337ValAsn: 2.337 ± 0.363
4.022ValPro: 4.022 ± 0.509
3.207ValGln: 3.207 ± 0.331
5.49ValArg: 5.49 ± 0.679
3.859ValSer: 3.859 ± 0.393
5.544ValThr: 5.544 ± 0.616
6.197ValVal: 6.197 ± 0.669
1.902ValTrp: 1.902 ± 0.271
1.902ValTyr: 1.902 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
2.718TrpAla: 2.718 ± 0.357
0.272TrpCys: 0.272 ± 0.11
1.141TrpAsp: 1.141 ± 0.268
1.033TrpGlu: 1.033 ± 0.253
0.707TrpPhe: 0.707 ± 0.18
0.87TrpGly: 0.87 ± 0.238
0.707TrpHis: 0.707 ± 0.257
0.978TrpIle: 0.978 ± 0.223
0.489TrpLys: 0.489 ± 0.225
2.555TrpLeu: 2.555 ± 0.406
0.707TrpMet: 0.707 ± 0.19
0.435TrpAsn: 0.435 ± 0.19
1.25TrpPro: 1.25 ± 0.264
0.924TrpGln: 0.924 ± 0.223
1.794TrpArg: 1.794 ± 0.315
1.359TrpSer: 1.359 ± 0.282
1.141TrpThr: 1.141 ± 0.233
1.196TrpVal: 1.196 ± 0.267
0.435TrpTrp: 0.435 ± 0.178
0.38TrpTyr: 0.38 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.446TyrAla: 2.446 ± 0.515
0.054TyrCys: 0.054 ± 0.053
1.576TyrAsp: 1.576 ± 0.339
1.576TyrGlu: 1.576 ± 0.33
0.707TyrPhe: 0.707 ± 0.173
2.011TyrGly: 2.011 ± 0.262
0.38TyrHis: 0.38 ± 0.144
0.489TyrIle: 0.489 ± 0.178
0.272TyrLys: 0.272 ± 0.117
2.283TyrLeu: 2.283 ± 0.411
0.544TyrMet: 0.544 ± 0.133
0.435TyrAsn: 0.435 ± 0.16
1.141TyrPro: 1.141 ± 0.28
0.598TyrGln: 0.598 ± 0.185
1.957TyrArg: 1.957 ± 0.36
1.196TyrSer: 1.196 ± 0.199
1.196TyrThr: 1.196 ± 0.323
1.957TyrVal: 1.957 ± 0.341
0.435TyrTrp: 0.435 ± 0.145
0.435TyrTyr: 0.435 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (18398 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski