Indibacter alkaliphilus LW1
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4650 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S2DM90|S2DM90_9BACT Uncharacterized protein OS=Indibacter alkaliphilus LW1 OX=1189612 GN=A33Q_0106 PE=4 SV=1
MM1 pKa = 7.84 RR2 pKa = 11.84 KK3 pKa = 9.22 LLKK6 pKa = 10.38 YY7 pKa = 10.39 LLVVLPFVVMAACVDD22 pKa = 3.66 EE23 pKa = 4.9 QNIEE27 pKa = 4.16 DD28 pKa = 4.67 LPPTSEE34 pKa = 4.31 DD35 pKa = 2.91 ATFSVEE41 pKa = 4.14 PTSEE45 pKa = 3.81 NPNIVNFTADD55 pKa = 3.26 RR56 pKa = 11.84 EE57 pKa = 4.32 FFRR60 pKa = 11.84 MQWDD64 pKa = 4.08 LGNGTTVEE72 pKa = 4.1 GRR74 pKa = 11.84 TVTGTYY80 pKa = 9.82 PNEE83 pKa = 3.72 GTYY86 pKa = 10.03 TVTLTVFNAAGSASFSQDD104 pKa = 2.32 IVIDD108 pKa = 3.68 QTDD111 pKa = 3.83 PTLLDD116 pKa = 3.49 SPIFNILTGGINAIEE131 pKa = 4.27 GRR133 pKa = 11.84 TWVIDD138 pKa = 3.6 SARR141 pKa = 11.84 VGHH144 pKa = 6.51 FGVGPNPSQAGDD156 pKa = 3.56 FPEE159 pKa = 4.8 WYY161 pKa = 9.86 AAQANEE167 pKa = 3.85 KK168 pKa = 9.27 RR169 pKa = 11.84 GSGMYY174 pKa = 8.93 TDD176 pKa = 5.11 RR177 pKa = 11.84 YY178 pKa = 9.18 TFFLDD183 pKa = 3.25 NFGFNMEE190 pKa = 4.53 TNGFVYY196 pKa = 10.49 LNEE199 pKa = 4.09 AQGPNFPGAFDD210 pKa = 4.56 PGVGDD215 pKa = 4.27 LSAPYY220 pKa = 8.61 EE221 pKa = 4.41 APDD224 pKa = 3.72 GLTWSYY230 pKa = 11.42 TEE232 pKa = 4.09 VEE234 pKa = 4.16 NGFDD238 pKa = 3.56 EE239 pKa = 5.13 LRR241 pKa = 11.84 ISEE244 pKa = 4.32 GGFLGYY250 pKa = 9.96 FAGGTTYY257 pKa = 10.63 QIVNLEE263 pKa = 4.12 EE264 pKa = 4.7 NEE266 pKa = 4.03 MFLRR270 pKa = 11.84 YY271 pKa = 8.81 IDD273 pKa = 3.64 QANEE277 pKa = 3.33 EE278 pKa = 4.32 LAWYY282 pKa = 9.4 IRR284 pKa = 11.84 LIPAGFDD291 pKa = 3.12 SGEE294 pKa = 4.08 DD295 pKa = 3.59 VQEE298 pKa = 4.36 EE299 pKa = 4.52 EE300 pKa = 4.43 PSDD303 pKa = 3.63 VNFNFEE309 pKa = 4.58 DD310 pKa = 4.55 LIGDD314 pKa = 4.47 GTQAWTLKK322 pKa = 10.11 SAAGAFGVGPAPGNDD337 pKa = 2.81 SFFPNGNDD345 pKa = 3.0 ISDD348 pKa = 3.64 EE349 pKa = 4.13 RR350 pKa = 11.84 ACLFNDD356 pKa = 4.49 LYY358 pKa = 10.77 IFSEE362 pKa = 4.51 DD363 pKa = 2.87 GTYY366 pKa = 10.33 EE367 pKa = 4.11 YY368 pKa = 8.98 DD369 pKa = 3.47 TQGDD373 pKa = 3.77 IFAEE377 pKa = 5.12 FYY379 pKa = 10.35 MGVSEE384 pKa = 5.44 EE385 pKa = 4.48 GCQDD389 pKa = 3.27 EE390 pKa = 5.06 SNLEE394 pKa = 4.12 GTPGEE399 pKa = 3.96 AWGSGVHH406 pKa = 5.96 SFEE409 pKa = 4.12 FTPGEE414 pKa = 4.23 GSTRR418 pKa = 11.84 PKK420 pKa = 9.51 ITVTGTGAFVVLAKK434 pKa = 10.58 AFNGGEE440 pKa = 4.13 YY441 pKa = 10.3 EE442 pKa = 4.2 QGPPNEE448 pKa = 4.46 NASVTYY454 pKa = 10.44 DD455 pKa = 3.39 VLDD458 pKa = 3.83 YY459 pKa = 11.25 DD460 pKa = 4.48 PEE462 pKa = 4.65 SKK464 pKa = 10.58 EE465 pKa = 3.77 LTLTIDD471 pKa = 3.13 ITNDD475 pKa = 2.92 GGVWWTFVLVPNEE488 pKa = 3.75
Molecular weight: 53.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.503
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.579
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.592
Sillero 3.821
Patrickios 1.049
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|S2DKF0|S2DKF0_9BACT mRNA interferase OS=Indibacter alkaliphilus LW1 OX=1189612 GN=A33Q_0549 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.78 HH16 pKa = 3.85 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.41 SRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.19 GRR39 pKa = 11.84 HH40 pKa = 5.41 KK41 pKa = 10.43 LTVSSEE47 pKa = 4.01 KK48 pKa = 9.88 TLKK51 pKa = 10.51 KK52 pKa = 10.72
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.471
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4650
0
4650
1482599
37
2932
318.8
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.411 ± 0.032
0.659 ± 0.011
5.43 ± 0.029
7.236 ± 0.036
5.524 ± 0.031
7.04 ± 0.04
1.856 ± 0.017
7.31 ± 0.03
6.872 ± 0.05
9.757 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.017
5.296 ± 0.034
3.872 ± 0.023
3.619 ± 0.021
4.149 ± 0.027
6.548 ± 0.028
4.781 ± 0.023
6.154 ± 0.031
1.255 ± 0.016
3.768 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here