Chondrus crispus (Carrageen Irish moss) (Polymorpha crispa)
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9598 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7QPH3|R7QPH3_CHOCR Symplekin-like OS=Chondrus crispus OX=2769 GN=CHC_T00010348001 PE=4 SV=1
MM1 pKa = 7.47 EE2 pKa = 4.63 WNEE5 pKa = 3.9 LVEE8 pKa = 4.99 GSDD11 pKa = 4.77 LEE13 pKa = 5.28 ADD15 pKa = 4.34 GYY17 pKa = 6.96 TTFIEE22 pKa = 4.48 VQVNRR27 pKa = 11.84 TLAPAVSLIAPTGPFGTQGTDD48 pKa = 3.13 QVVVYY53 pKa = 9.55 IDD55 pKa = 3.6 NVAEE59 pKa = 4.57 GEE61 pKa = 4.21 DD62 pKa = 5.18 DD63 pKa = 3.9 PTSWTYY69 pKa = 11.48 DD70 pKa = 3.45 LSILFADD77 pKa = 4.85 GAKK80 pKa = 10.12 SATVDD85 pKa = 3.74 TPAVTQFGNGTISVAFTLPAGSGVDD110 pKa = 4.05 LPWNLTITKK119 pKa = 9.27 PDD121 pKa = 3.45 AQVVPVVDD129 pKa = 3.48 ATNPSFLFSFVSQLSVIDD147 pKa = 3.97 IQPSSGPEE155 pKa = 3.5 EE156 pKa = 4.4 GGITVSLIGTFPGFDD171 pKa = 3.37 TEE173 pKa = 4.64 AVDD176 pKa = 5.1 VLFDD180 pKa = 4.13 GNPINPEE187 pKa = 4.72 LITVIGPEE195 pKa = 4.47 NITFTLPPKK204 pKa = 10.46 VSIGTEE210 pKa = 3.48 FDD212 pKa = 3.74 VNVTVSIGAQVTSGITFSYY231 pKa = 9.98 IPSILLQSLTPASGPVEE248 pKa = 4.57 GGTQVTLVGQFIDD261 pKa = 3.95 FTSDD265 pKa = 2.57 IDD267 pKa = 3.67 GTGIYY272 pKa = 10.35 FGGEE276 pKa = 4.19 KK277 pKa = 9.17 IDD279 pKa = 3.95 SDD281 pKa = 5.7 LITASNSSVIVFTTPPQSFFASEE304 pKa = 3.8 SVYY307 pKa = 10.64 AYY309 pKa = 10.22 DD310 pKa = 3.69 VSVHH314 pKa = 6.13 IGNSSSNSIVFSYY327 pKa = 10.35 DD328 pKa = 2.74 APLQITSIAPNSGTEE343 pKa = 3.91 EE344 pKa = 4.41 GGTDD348 pKa = 2.92 ITLGGTFQAFDD359 pKa = 3.92 LSNSNVYY366 pKa = 10.11 IGSTKK371 pKa = 9.54 IDD373 pKa = 3.23 SSTIVHH379 pKa = 6.75 NSTSILFITPPRR391 pKa = 11.84 EE392 pKa = 3.97 EE393 pKa = 4.02 VGNGYY398 pKa = 7.91 TYY400 pKa = 10.52 NIWITIEE407 pKa = 4.25 TTSSNGTSTTITSNTVTFTYY427 pKa = 10.47 EE428 pKa = 3.96 DD429 pKa = 3.26 VGSQVSIDD437 pKa = 3.49 ASGGSFNSDD446 pKa = 2.04 GHH448 pKa = 6.85 YY449 pKa = 10.89 EE450 pKa = 4.08 LGQCVEE456 pKa = 4.51 SLYY459 pKa = 10.35 QASISRR465 pKa = 11.84 GARR468 pKa = 11.84 LQNASYY474 pKa = 10.35 RR475 pKa = 11.84 WSLVANDD482 pKa = 4.3 TEE484 pKa = 4.4 TDD486 pKa = 3.13 ILSAKK491 pKa = 10.46 GIVTNSDD498 pKa = 3.58 VLLLPYY504 pKa = 10.0 TVFSEE509 pKa = 4.24 EE510 pKa = 3.62 NAAYY514 pKa = 9.0 TLTLTVDD521 pKa = 3.12 TDD523 pKa = 4.12 FFSFTKK529 pKa = 10.36 TLVLTQLSSQNIGVRR544 pKa = 11.84 IKK546 pKa = 10.57 NPRR549 pKa = 11.84 PRR551 pKa = 11.84 SISGPNTTLTIPADD565 pKa = 4.15 LYY567 pKa = 11.67 LPGCQGNVIIVNSTEE582 pKa = 3.81 MTYY585 pKa = 10.11 EE586 pKa = 3.67 WVFRR590 pKa = 11.84 GEE592 pKa = 4.48 TYY594 pKa = 10.9 VFSHH598 pKa = 5.93 QNEE601 pKa = 4.5 SAPEE605 pKa = 3.94 EE606 pKa = 4.18 QASPTLLGRR615 pKa = 11.84 EE616 pKa = 3.78 FHH618 pKa = 6.74 IPQAVMEE625 pKa = 4.38 YY626 pKa = 10.25 GSFGISLTAYY636 pKa = 8.23 FTDD639 pKa = 3.49 QPSIKK644 pKa = 10.63 GIDD647 pKa = 3.4 TSTVVINPAALISQINGGEE666 pKa = 3.83 ASQLISEE673 pKa = 4.45 TGKK676 pKa = 10.13 FHH678 pKa = 6.94 SLWFTIPRR686 pKa = 11.84 SS687 pKa = 3.52
Molecular weight: 73.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.125
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|R7Q251|R7Q251_CHOCR Uncharacterized protein OS=Chondrus crispus OX=2769 GN=CHC_T00001368001 PE=4 SV=1
MM1 pKa = 7.47 TKK3 pKa = 9.12 GTNRR7 pKa = 11.84 KK8 pKa = 9.3 KK9 pKa = 9.82 IRR11 pKa = 11.84 KK12 pKa = 8.2 AGFRR16 pKa = 11.84 ARR18 pKa = 11.84 MKK20 pKa = 8.98 TVSGRR25 pKa = 11.84 RR26 pKa = 11.84 IIKK29 pKa = 9.51 ARR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 8.44 KK35 pKa = 10.03 RR36 pKa = 11.84 KK37 pKa = 9.82 RR38 pKa = 11.84 IGLL41 pKa = 3.8
Molecular weight: 4.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.272
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.778
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.501
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9598
0
9598
3411166
10
5324
355.4
39.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.083 ± 0.027
1.749 ± 0.015
5.399 ± 0.022
6.123 ± 0.033
3.618 ± 0.019
6.788 ± 0.028
2.531 ± 0.015
4.332 ± 0.02
4.763 ± 0.024
8.995 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.328 ± 0.011
3.295 ± 0.017
5.519 ± 0.04
3.446 ± 0.02
7.486 ± 0.026
8.141 ± 0.034
5.424 ± 0.019
7.021 ± 0.022
1.171 ± 0.009
2.315 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here