Endozoicomonas numazuensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Endozoicomonadaceae; Endozoicomonas

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081NL42|A0A081NL42_9GAMM Uncharacterized protein OS=Endozoicomonas numazuensis OX=1137799 GN=GZ78_03980 PE=4 SV=1
MM1 pKa = 7.38LSSWLCFLLLIFSLPVIALDD21 pKa = 4.03LDD23 pKa = 3.82GWDD26 pKa = 3.89EE27 pKa = 4.1LSAGEE32 pKa = 4.54GNTASVWQSGADD44 pKa = 3.36QNLTLEE50 pKa = 4.33QSGEE54 pKa = 4.16ANFFRR59 pKa = 11.84SAQFDD64 pKa = 4.06FNNNLKK70 pKa = 10.61ASQNGFANSITAIQRR85 pKa = 11.84GSGNQASISQTGDD98 pKa = 3.14YY99 pKa = 10.38NDD101 pKa = 3.65VVMHH105 pKa = 6.45QSGFDD110 pKa = 3.27NYY112 pKa = 11.41SEE114 pKa = 4.02ITQVGSFNVAEE125 pKa = 4.07LTQLGEE131 pKa = 4.13NNSAYY136 pKa = 9.72IIQNGYY142 pKa = 8.68GLSVSVIQSGNQNVQIEE159 pKa = 4.4QNNN162 pKa = 3.39

Molecular weight: 17.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081NE92|A0A081NE92_9GAMM Peptidoglycan D D-transpeptidase FtsI OS=Endozoicomonas numazuensis OX=1137799 GN=ftsI PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4400

0

4400

1400203

40

4265

318.2

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.016 ± 0.04

1.112 ± 0.015

5.589 ± 0.038

6.771 ± 0.041

3.966 ± 0.027

6.943 ± 0.04

2.291 ± 0.018

5.772 ± 0.029

5.393 ± 0.039

10.466 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.022

3.97 ± 0.028

4.347 ± 0.029

4.504 ± 0.031

5.09 ± 0.033

7.134 ± 0.044

5.23 ± 0.038

6.609 ± 0.03

1.266 ± 0.014

2.941 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski