Aspergillus versicolor CBS 583.65
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13222 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9PMJ3|A0A1L9PMJ3_ASPVE Uncharacterized protein OS=Aspergillus versicolor CBS 583.65 OX=1036611 GN=ASPVEDRAFT_131628 PE=4 SV=1
MM1 pKa = 6.74 THH3 pKa = 5.71 STLGSLYY10 pKa = 10.74 LFLAPILSVLAQDD23 pKa = 5.33 LPADD27 pKa = 3.7 LTGCTEE33 pKa = 3.94 VDD35 pKa = 4.12 CPNDD39 pKa = 3.23 GWDD42 pKa = 3.43 SCTVADD48 pKa = 4.2 EE49 pKa = 4.62 TYY51 pKa = 11.14 AGIGLSGIDD60 pKa = 4.17 SVPDD64 pKa = 3.51 SLDD67 pKa = 3.88 GISLVKK73 pKa = 10.25 GVHH76 pKa = 5.8 VEE78 pKa = 4.03 DD79 pKa = 5.71 DD80 pKa = 3.81 EE81 pKa = 6.23 DD82 pKa = 6.29 RR83 pKa = 11.84 GDD85 pKa = 3.77 TDD87 pKa = 3.37 NGADD91 pKa = 3.16 KK92 pKa = 10.38 TRR94 pKa = 11.84 PFRR97 pKa = 11.84 SVYY100 pKa = 10.84 YY101 pKa = 10.41 LGTPANVNVDD111 pKa = 4.19 DD112 pKa = 5.19 LSGCAVVFNDD122 pKa = 4.34 PPTGHH127 pKa = 6.46 FNKK130 pKa = 10.16 PLINDD135 pKa = 3.67 SVNVNTRR142 pKa = 11.84 AAYY145 pKa = 7.08 GTCPDD150 pKa = 4.94 VIQPDD155 pKa = 4.7 CIDD158 pKa = 3.95 KK159 pKa = 9.36 LTEE162 pKa = 3.68 QARR165 pKa = 11.84 KK166 pKa = 7.28 TKK168 pKa = 10.78 YY169 pKa = 10.09 NGSSPCSTLQSALQDD184 pKa = 3.81 NPPDD188 pKa = 3.68 EE189 pKa = 5.71 CSDD192 pKa = 3.57 LTGGGDD198 pKa = 3.67 GLGKK202 pKa = 10.21 FDD204 pKa = 5.61 VISLGNLSTISKK216 pKa = 10.05 SDD218 pKa = 3.64 NASSDD223 pKa = 3.6 CWPVLPKK230 pKa = 10.73 SNNLAQLTDD239 pKa = 3.61 DD240 pKa = 3.85 TAMGNYY246 pKa = 8.5 TNEE249 pKa = 3.84 GLLAEE254 pKa = 4.75 MYY256 pKa = 10.39 KK257 pKa = 8.78 ITPVLTVFTSGNNSNSLVNEE277 pKa = 4.5 TSASLTCLKK286 pKa = 10.78 VVTTPNIDD294 pKa = 3.27 NGTDD298 pKa = 3.28 ADD300 pKa = 4.11 TDD302 pKa = 3.77 TDD304 pKa = 3.46 TGAAALSPANMVAVTLGALATAAFALLL331 pKa = 3.78
Molecular weight: 34.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.266
Thurlkill 3.656
EMBOSS 3.821
Sillero 3.961
Patrickios 1.278
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A1L9PC49|A0A1L9PC49_ASPVE Uncharacterized protein OS=Aspergillus versicolor CBS 583.65 OX=1036611 GN=ASPVEDRAFT_25939 PE=4 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.41 SFRR8 pKa = 11.84 TKK10 pKa = 10.38 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13222
0
13222
6349617
49
9209
480.2
53.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.448 ± 0.017
1.341 ± 0.007
5.635 ± 0.015
5.999 ± 0.02
3.861 ± 0.012
7.028 ± 0.018
2.396 ± 0.009
5.082 ± 0.013
4.399 ± 0.015
9.147 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.009
3.661 ± 0.01
5.982 ± 0.022
3.965 ± 0.015
6.042 ± 0.018
8.256 ± 0.022
5.918 ± 0.014
6.273 ± 0.014
1.542 ± 0.008
2.913 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here