Desulforhopalus singaporensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulforhopalus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0R7U2|A0A1H0R7U2_9DELT Protein HflK OS=Desulforhopalus singaporensis OX=91360 GN=SAMN05660330_02210 PE=3 SV=1
DDD2 pKa = 3.94EE3 pKa = 4.43TTRR6 pKa = 11.84GEEE9 pKa = 3.56DDD11 pKa = 3.64YY12 pKa = 10.18YYY14 pKa = 11.14KK15 pKa = 10.91QIVDDD20 pKa = 3.19KK21 pKa = 10.37GNVGNVDDD29 pKa = 3.79QDDD32 pKa = 3.28TIDDD36 pKa = 3.61SGPQDDD42 pKa = 3.46TIDDD46 pKa = 3.28TGITEEE52 pKa = 4.07DD53 pKa = 3.74GLVADDD59 pKa = 5.62DD60 pKa = 5.19YY61 pKa = 9.79YY62 pKa = 11.4NDDD65 pKa = 3.17TLVVSGVLGVALLAGEEE82 pKa = 4.41VQVQLDDD89 pKa = 3.25GNWYYY94 pKa = 7.66ATIDDD99 pKa = 3.54TGTGWSFDDD108 pKa = 3.92SSTTLDDD115 pKa = 3.43EE116 pKa = 4.77DDD118 pKa = 3.84HH119 pKa = 5.85FNAKKK124 pKa = 9.09VNAAGNDDD132 pKa = 3.6ATTSKKK138 pKa = 9.85VTVDDD143 pKa = 3.32TADDD147 pKa = 3.51AVAEEE152 pKa = 4.41TSIADDD158 pKa = 3.37DD159 pKa = 3.65GMVTDDD165 pKa = 5.62DD166 pKa = 4.25VTNDDD171 pKa = 3.27TLTVYYY177 pKa = 8.7TLDDD181 pKa = 3.12KK182 pKa = 11.31LGEEE186 pKa = 4.03SYYY189 pKa = 10.57EEE191 pKa = 3.6WGARR195 pKa = 11.84EEE197 pKa = 3.54QTPVKKK203 pKa = 10.18GVATVDDD210 pKa = 3.42TGTGWSFDDD219 pKa = 3.59SGNPHHH225 pKa = 6.77EE226 pKa = 5.41DD227 pKa = 3.14EEE229 pKa = 4.5YY230 pKa = 9.33YYY232 pKa = 10.1YYY234 pKa = 8.5EE235 pKa = 5.6RR236 pKa = 11.84VFDDD240 pKa = 3.49GGNSGSVDDD249 pKa = 3.52KKK251 pKa = 11.22VQIDDD256 pKa = 3.64VGPEEE261 pKa = 4.09LNLDDD266 pKa = 3.3TFEEE270 pKa = 4.34LTDDD274 pKa = 4.02YYY276 pKa = 11.0HH277 pKa = 7.68LDDD280 pKa = 5.06DDD282 pKa = 4.43EEE284 pKa = 4.71DD285 pKa = 4.45VSGINNDDD293 pKa = 4.54ITRR296 pKa = 11.84DDD298 pKa = 3.72YY299 pKa = 10.76ITIAGEEE306 pKa = 4.04SSSLQAGEEE315 pKa = 4.26YY316 pKa = 10.09QISTDDD322 pKa = 3.58EEE324 pKa = 3.98WEEE327 pKa = 4.67LLQDDD332 pKa = 4.79GKKK335 pKa = 9.08WSWKKK340 pKa = 10.11VDDD343 pKa = 3.16DDD345 pKa = 4.07YY346 pKa = 11.56VDDD349 pKa = 3.55TTTYYY354 pKa = 11.4YY355 pKa = 10.92RR356 pKa = 11.84LVDDD360 pKa = 3.85AGNEEE365 pKa = 4.04TGTVFKKK372 pKa = 10.29DDD374 pKa = 2.54GYYY377 pKa = 10.17EE378 pKa = 4.12TVDDD382 pKa = 3.62TAPDDD387 pKa = 5.56ILVAPGGVPEEE398 pKa = 4.34TSTTEEE404 pKa = 3.49EE405 pKa = 4.06TFDDD409 pKa = 3.13NTYYY413 pKa = 10.17KKK415 pKa = 10.19EEE417 pKa = 4.21NCYYY421 pKa = 8.1ALYYY425 pKa = 10.74DDD427 pKa = 3.59ANDDD431 pKa = 3.56SYYY434 pKa = 10.93LGVDDD439 pKa = 3.62AIHHH443 pKa = 5.38YY444 pKa = 7.9QADDD448 pKa = 3.38SGNWSMTTKKK458 pKa = 10.81PAGSNNLTFAVWDDD472 pKa = 3.68EE473 pKa = 4.17GNRR476 pKa = 11.84SQFGPSTSVGVSEEE490 pKa = 4.58TGSEEE495 pKa = 4.7DDD497 pKa = 3.45LTWGGTVNPGLGLNSAAVTINEEE520 pKa = 4.32DD521 pKa = 3.76LWSFFQSSAALSGTTTANAGWVFAAKKK548 pKa = 10.44QDDD551 pKa = 3.76DD552 pKa = 4.06YY553 pKa = 11.98ATYYY557 pKa = 10.77EEE559 pKa = 4.22PRR561 pKa = 11.84DDD563 pKa = 3.82TSAAYYY569 pKa = 9.85EE570 pKa = 4.11FVNAALFADDD580 pKa = 4.24YY581 pKa = 11.14RR582 pKa = 11.84DDD584 pKa = 2.95YYY586 pKa = 11.63DDD588 pKa = 4.08ISQVSSYYY596 pKa = 11.46NASRR600 pKa = 11.84TAMWTNNGDDD610 pKa = 3.43TYYY613 pKa = 9.91PGSINQGTLNHHH625 pKa = 6.72GGAIAYYY632 pKa = 9.03DD633 pKa = 3.84KK634 pKa = 11.43EE635 pKa = 4.33DDD637 pKa = 3.72YYY639 pKa = 11.47DDD641 pKa = 3.96VLADDD646 pKa = 3.86EEE648 pKa = 4.4DDD650 pKa = 3.32LSFLKKK656 pKa = 10.97VDDD659 pKa = 3.81DD660 pKa = 4.75SGGRR664 pKa = 11.84KKK666 pKa = 7.17TYYY669 pKa = 8.53EE670 pKa = 4.14NAGYYY675 pKa = 10.84YY676 pKa = 9.95GVPAGVDDD684 pKa = 2.92YYY686 pKa = 11.04SILHHH691 pKa = 5.75EE692 pKa = 4.38SAVDDD697 pKa = 4.4DDD699 pKa = 3.93DDD701 pKa = 3.84SVDDD705 pKa = 3.38AAHHH709 pKa = 5.36DDD711 pKa = 3.18LGGGNPGRR719 pKa = 11.84VLTVFHHH726 pKa = 7.07EEE728 pKa = 3.94NDDD731 pKa = 3.59SSQQFTMQDDD741 pKa = 3.32YY742 pKa = 9.91NVFRR746 pKa = 11.84NDDD749 pKa = 3.21YYY751 pKa = 11.61DDD753 pKa = 3.79YY754 pKa = 11.45NLSYYY759 pKa = 11.81MTWGDDD765 pKa = 3.75YY766 pKa = 11.2DD767 pKa = 4.22DDD769 pKa = 4.22YYY771 pKa = 11.66DDD773 pKa = 4.19FLSRR777 pKa = 11.84GNIKKK782 pKa = 10.67GVGNSDDD789 pKa = 3.29EE790 pKa = 4.19RR791 pKa = 11.84IYYY794 pKa = 10.92NDDD797 pKa = 3.61SGGLKKK803 pKa = 9.12ATEEE807 pKa = 4.48DD808 pKa = 3.7TWMGDDD814 pKa = 2.87LAGGTSFAVDDD825 pKa = 3.49NHHH828 pKa = 7.16DD829 pKa = 3.31LMDDD833 pKa = 4.4VEEE836 pKa = 4.8PNSRR840 pKa = 11.84AVQFGPTLYYY850 pKa = 10.68NQGDDD855 pKa = 4.49TWEEE859 pKa = 3.81MTMTEEE865 pKa = 4.29LGSTITGGVALDDD878 pKa = 4.13YY879 pKa = 11.05WDDD882 pKa = 3.46AMDDD886 pKa = 4.42VLYYY890 pKa = 10.18PSSTSLQSGADDD902 pKa = 3.71NTAPSKKK909 pKa = 10.61VNNTNIAADDD919 pKa = 3.94TSLQLRR925 pKa = 11.84ILDDD929 pKa = 3.77EEE931 pKa = 4.32MNVYYY936 pKa = 10.3YY937 pKa = 10.98NTVKKK942 pKa = 10.82YYY944 pKa = 10.73DD945 pKa = 3.53AGNCVATTLMNPQGSGSSNAMGLVSFYYY973 pKa = 11.33LDDD976 pKa = 3.42DDD978 pKa = 3.97EE979 pKa = 5.01YYY981 pKa = 11.03VQLLRR986 pKa = 11.84TTNGEEE992 pKa = 3.91DDD994 pKa = 3.23HH995 pKa = 6.62GSVASQGGFTNSTVNSSWGGLTTGKKK1021 pKa = 9.85HHH1023 pKa = 7.78DD1024 pKa = 3.68YYY1026 pKa = 10.05LVGEEE1031 pKa = 4.77DDD1033 pKa = 3.68STASSTNEEE1042 pKa = 3.3GIVGSGYYY1050 pKa = 10.46DDD1052 pKa = 3.53FVSSAGDDD1060 pKa = 3.75DD1061 pKa = 3.32FNGSGGWSQLVISDDD1076 pKa = 4.57VWEEE1080 pKa = 4.24DDD1082 pKa = 3.56GLDDD1086 pKa = 3.22VDDD1089 pKa = 3.43YY1090 pKa = 11.22KKK1092 pKa = 10.24TGGGVQANLATGVVTGAAGNDDD1114 pKa = 3.36LINIEEE1120 pKa = 4.19LLGTSADDD1128 pKa = 3.86DD1129 pKa = 3.74FTDDD1133 pKa = 3.43AANNVFEEE1141 pKa = 4.5RR1142 pKa = 11.84GGNDDD1147 pKa = 3.05YYY1149 pKa = 11.27LTGGGRR1155 pKa = 11.84DDD1157 pKa = 3.31LLYYY1161 pKa = 10.35EE1162 pKa = 4.23LADDD1166 pKa = 4.12DDD1168 pKa = 4.05TGGNGSDDD1176 pKa = 4.24INDDD1180 pKa = 3.56TVGLVKKK1187 pKa = 10.55DD1188 pKa = 3.85ADDD1191 pKa = 4.18DDD1193 pKa = 4.22INISDDD1199 pKa = 4.06LNYYY1203 pKa = 9.84GTVGTYYY1210 pKa = 8.8DDD1212 pKa = 3.21SGTYYY1217 pKa = 8.56HH1218 pKa = 7.83DDD1220 pKa = 3.42ASEEE1224 pKa = 4.2VNDDD1228 pKa = 4.24VKKK1231 pKa = 9.88TTSGGNTEEE1240 pKa = 3.91AIDDD1244 pKa = 3.61DDD1246 pKa = 3.5AGNYYY1251 pKa = 6.54TLVTLEEE1258 pKa = 4.04TEEE1261 pKa = 4.04DDD1263 pKa = 3.96LSLLTNQQLIVEEE1276 pKa = 4.64SADDD1280 pKa = 3.51GASGAAASQQEEE1292 pKa = 4.37DD1293 pKa = 4.24EEE1295 pKa = 4.68STGSTGGDDD1304 pKa = 3.72DD1305 pKa = 3.79TASDDD1310 pKa = 4.75STTAPIADDD1319 pKa = 3.83SGNTTDDD1326 pKa = 3.24SGVVDDD1332 pKa = 3.9ADDD1335 pKa = 3.25VTTRR1339 pKa = 11.84VEEE1342 pKa = 4.34DD1343 pKa = 4.67DD1344 pKa = 5.04VTQLNSPTVI

Molecular weight: 142.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0J2U3|A0A1H0J2U3_9DELT Cu+-exporting ATPase OS=Desulforhopalus singaporensis OX=91360 GN=SAMN05660330_00122 PE=3 SV=1
MM1 pKa = 7.48AKK3 pKa = 9.86KK4 pKa = 9.04RR5 pKa = 11.84TYY7 pKa = 10.26RR8 pKa = 11.84VVDD11 pKa = 3.23GRR13 pKa = 11.84MNFVCPEE20 pKa = 3.78CHH22 pKa = 5.51TRR24 pKa = 11.84RR25 pKa = 11.84LVGVSPHH32 pKa = 6.09LKK34 pKa = 9.55NRR36 pKa = 11.84SIKK39 pKa = 9.26CHH41 pKa = 5.79KK42 pKa = 9.87CSTVVSCTLNRR53 pKa = 11.84RR54 pKa = 11.84EE55 pKa = 4.49TIRR58 pKa = 11.84EE59 pKa = 3.85QQFGKK64 pKa = 10.78VLLTRR69 pKa = 11.84YY70 pKa = 9.35DD71 pKa = 3.55RR72 pKa = 11.84SVVEE76 pKa = 4.64VNLLDD81 pKa = 3.49ISTWGVGFEE90 pKa = 4.37LPFRR94 pKa = 11.84EE95 pKa = 4.31LKK97 pKa = 10.28KK98 pKa = 10.79LSVGEE103 pKa = 4.26RR104 pKa = 11.84IEE106 pKa = 5.76FRR108 pKa = 11.84CPWNPKK114 pKa = 9.95LLGSSAYY121 pKa = 10.03VIRR124 pKa = 11.84AINGRR129 pKa = 11.84RR130 pKa = 11.84VGAEE134 pKa = 3.95RR135 pKa = 11.84LRR137 pKa = 11.84KK138 pKa = 9.45SS139 pKa = 3.13

Molecular weight: 16.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4348

0

4348

1432539

15

1863

329.5

36.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.161 ± 0.036

1.461 ± 0.017

5.435 ± 0.03

6.358 ± 0.034

4.284 ± 0.028

7.795 ± 0.037

2.031 ± 0.016

6.739 ± 0.031

5.615 ± 0.032

9.987 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.017

3.759 ± 0.021

4.197 ± 0.022

3.362 ± 0.023

5.392 ± 0.035

6.067 ± 0.024

5.346 ± 0.025

7.229 ± 0.029

1.073 ± 0.014

3.032 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski