Oceanisphaera psychrotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Aeromonadaceae; Oceanisphaera

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3066 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J4QFM6|A0A1J4QFM6_9GAMM Aspartate 1-decarboxylase OS=Oceanisphaera psychrotolerans OX=1414654 GN=panD PE=3 SV=1
MM1 pKa = 8.36DD2 pKa = 3.87NRR4 pKa = 11.84LWSLCFLDD12 pKa = 4.18EE13 pKa = 4.36GVALSVISRR22 pKa = 11.84KK23 pKa = 7.43EE24 pKa = 4.01TRR26 pKa = 11.84CQWLSDD32 pKa = 3.56EE33 pKa = 4.52DD34 pKa = 3.79QARR37 pKa = 11.84HH38 pKa = 5.23YY39 pKa = 10.84LLNEE43 pKa = 4.06YY44 pKa = 9.07LQHH47 pKa = 5.42VAEE50 pKa = 4.96LGEE53 pKa = 4.6LDD55 pKa = 3.74EE56 pKa = 4.32QQTLVARR63 pKa = 11.84EE64 pKa = 3.8RR65 pKa = 11.84FEE67 pKa = 5.75LLMEE71 pKa = 4.31QYY73 pKa = 10.17PEE75 pKa = 4.38PEE77 pKa = 4.06TLVEE81 pKa = 4.09YY82 pKa = 11.15LNDD85 pKa = 3.53LTSGLTRR92 pKa = 11.84ILWFGPLSALAEE104 pKa = 4.73DD105 pKa = 4.55YY106 pKa = 11.52SDD108 pKa = 3.62FALALRR114 pKa = 11.84AYY116 pKa = 8.64YY117 pKa = 9.47WEE119 pKa = 4.83EE120 pKa = 3.84YY121 pKa = 11.09GEE123 pKa = 4.77DD124 pKa = 4.17DD125 pKa = 4.91EE126 pKa = 7.01DD127 pKa = 4.13PATPVAEE134 pKa = 4.9DD135 pKa = 3.03DD136 pKa = 3.73WIYY139 pKa = 10.99LVEE142 pKa = 5.09AMDD145 pKa = 6.11DD146 pKa = 4.38FLLQDD151 pKa = 4.69DD152 pKa = 4.59YY153 pKa = 12.29

Molecular weight: 18.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J4QHW5|A0A1J4QHW5_9GAMM TPR_REGION domain-containing protein OS=Oceanisphaera psychrotolerans OX=1414654 GN=BFR47_08895 PE=4 SV=1
MM1 pKa = 7.38GLILGLIIGGLAGWIAGNLTKK22 pKa = 10.9GQGFGLLGNILVGLVGGFLGGVVFRR47 pKa = 11.84LLGLAATGVIGSLIMSVIGAVLLIFIVGKK76 pKa = 9.98IRR78 pKa = 11.84RR79 pKa = 11.84SRR81 pKa = 11.84SS82 pKa = 3.07

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3066

0

3066

968909

44

5148

316.0

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.192 ± 0.054

1.038 ± 0.016

5.384 ± 0.039

5.973 ± 0.041

3.708 ± 0.03

7.852 ± 0.052

2.361 ± 0.027

5.181 ± 0.041

3.533 ± 0.039

11.893 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.023

3.138 ± 0.029

4.599 ± 0.037

4.776 ± 0.041

6.101 ± 0.049

5.655 ± 0.033

4.984 ± 0.037

6.99 ± 0.035

1.392 ± 0.022

2.604 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski