Salinimonas sediminis
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3700 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346NHQ7|A0A346NHQ7_9ALTE Class A beta-lactamase-related serine hydrolase OS=Salinimonas sediminis OX=2303538 GN=D0Y50_00975 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 9.24 LTRR5 pKa = 11.84 YY6 pKa = 9.52 GVLGVMAGTVLAACGGGSDD25 pKa = 4.17 NSSSSTQNGSQTAGKK40 pKa = 9.68 DD41 pKa = 3.06 ASQIIGNASAHH52 pKa = 6.01 SVSEE56 pKa = 4.09 LNKK59 pKa = 9.94 AARR62 pKa = 11.84 SLASASYY69 pKa = 9.67 TGSQEE74 pKa = 3.99 KK75 pKa = 11.13 AEE77 pKa = 4.54 LDD79 pKa = 3.55 FLLAQQVTRR88 pKa = 11.84 MLLDD92 pKa = 4.65 DD93 pKa = 4.59 DD94 pKa = 4.21 VLRR97 pKa = 11.84 VPEE100 pKa = 4.26 IVTHH104 pKa = 5.76 EE105 pKa = 4.4 LSPDD109 pKa = 2.95 ARR111 pKa = 11.84 QDD113 pKa = 3.44 EE114 pKa = 4.93 NINATFDD121 pKa = 3.79 CDD123 pKa = 3.56 EE124 pKa = 5.14 GGSASYY130 pKa = 10.45 SGQLNEE136 pKa = 4.66 SGEE139 pKa = 4.29 ASLSLNFDD147 pKa = 3.47 NCKK150 pKa = 10.43 SYY152 pKa = 11.79 YY153 pKa = 9.96 NDD155 pKa = 3.43 SALTGVAALVIKK167 pKa = 10.84 LDD169 pKa = 3.67 VQQNTSAYY177 pKa = 8.74 TLYY180 pKa = 10.62 FDD182 pKa = 4.33 NLRR185 pKa = 11.84 WHH187 pKa = 7.45 DD188 pKa = 3.85 GQAVSLSGLMAFNEE202 pKa = 4.37 SADD205 pKa = 3.05 WEE207 pKa = 4.52 MPYY210 pKa = 7.92 TTLSQQYY217 pKa = 9.3 IALSVGNEE225 pKa = 4.07 TYY227 pKa = 11.18 LLDD230 pKa = 4.92 AEE232 pKa = 4.74 TTGEE236 pKa = 4.14 AEE238 pKa = 4.19 SGSLLEE244 pKa = 4.3 SMQGRR249 pKa = 11.84 LSVASRR255 pKa = 11.84 GYY257 pKa = 10.06 IDD259 pKa = 5.87 FSYY262 pKa = 10.87 EE263 pKa = 3.98 PQTYY267 pKa = 8.35 TYY269 pKa = 10.32 DD270 pKa = 3.82 FNGSTLFFNGNEE282 pKa = 3.9 AYY284 pKa = 11.09 VNFEE288 pKa = 3.46 QDD290 pKa = 2.47 TAAFVIDD297 pKa = 3.83 TDD299 pKa = 4.22 QDD301 pKa = 3.48 GTTDD305 pKa = 3.06 AGKK308 pKa = 10.03 ILSLADD314 pKa = 4.17 FFAPPPADD322 pKa = 3.47 ASLNPLTEE330 pKa = 4.28 ISSQPYY336 pKa = 9.91 AGEE339 pKa = 4.5 PYY341 pKa = 10.18 TNEE344 pKa = 3.36 SYY346 pKa = 9.61 YY347 pKa = 10.62 TVSTDD352 pKa = 2.79 IDD354 pKa = 3.34 ISPGYY359 pKa = 9.97 FFDD362 pKa = 4.33 IEE364 pKa = 4.51 TPRR367 pKa = 11.84 SQLEE371 pKa = 3.75 ISYY374 pKa = 9.88 NWYY377 pKa = 9.92 INGILLEE384 pKa = 4.47 GVHH387 pKa = 6.7 GSTLPAYY394 pKa = 10.09 SAGVHH399 pKa = 6.72 DD400 pKa = 5.23 EE401 pKa = 4.42 VTVSMVVSDD410 pKa = 3.69 ATHH413 pKa = 6.55 SYY415 pKa = 10.87 EE416 pKa = 5.78 SYY418 pKa = 10.04 QQYY421 pKa = 10.08 IWLEE425 pKa = 4.0 DD426 pKa = 3.55 MPAEE430 pKa = 4.09 LTVTDD435 pKa = 4.51 MPASIAAGEE444 pKa = 4.32 TVSFSVVLTDD454 pKa = 4.97 PDD456 pKa = 3.71 IEE458 pKa = 4.51 SADD461 pKa = 3.47 NLARR465 pKa = 11.84 LTNAPQGASMSEE477 pKa = 4.22 DD478 pKa = 3.19 GTVTWQVPQDD488 pKa = 3.92 LLFSEE493 pKa = 4.2 QNYY496 pKa = 9.19 YY497 pKa = 10.88 FGFSNRR503 pKa = 11.84 LNPNEE508 pKa = 4.33 TIEE511 pKa = 4.25 KK512 pKa = 9.34 TVTATVNASQPLPLFRR528 pKa = 11.84 SGIEE532 pKa = 3.7 VPAANRR538 pKa = 11.84 SQWVVDD544 pKa = 3.44 IDD546 pKa = 4.51 GDD548 pKa = 4.16 GQNEE552 pKa = 4.3 LLSTDD557 pKa = 2.88 NDD559 pKa = 3.22 QRR561 pKa = 11.84 VFLLSLDD568 pKa = 3.51 NGQYY572 pKa = 6.43 QQKK575 pKa = 8.31 WAYY578 pKa = 9.36 PYY580 pKa = 10.93 VLSQQEE586 pKa = 4.38 TIQQVLPIGRR596 pKa = 11.84 NQAGSKK602 pKa = 9.68 PILVVTDD609 pKa = 3.57 HH610 pKa = 6.77 ALWRR614 pKa = 11.84 IDD616 pKa = 5.49 DD617 pKa = 4.4 LAQPARR623 pKa = 11.84 QIFNTDD629 pKa = 2.56 NFLRR633 pKa = 11.84 SALLADD639 pKa = 3.56 IDD641 pKa = 4.21 GDD643 pKa = 3.93 GTEE646 pKa = 3.94 EE647 pKa = 4.91 LIYY650 pKa = 10.62 HH651 pKa = 7.1 AAADD655 pKa = 4.28 AYY657 pKa = 10.63 SATLAIVNVVALDD670 pKa = 4.08 DD671 pKa = 4.53 PSTEE675 pKa = 3.96 LMSFTVDD682 pKa = 2.88 EE683 pKa = 5.02 FSTFDD688 pKa = 3.39 VGNVDD693 pKa = 2.88 TDD695 pKa = 3.81 ANLEE699 pKa = 4.16 LVLNTGSVFDD709 pKa = 5.02 LVTAEE714 pKa = 4.82 NEE716 pKa = 4.09 WQLGTGFSTQYY727 pKa = 9.32 VTTGDD732 pKa = 3.9 LNGDD736 pKa = 4.14 GIDD739 pKa = 4.32 EE740 pKa = 5.0 IISADD745 pKa = 3.18 SWGAMYY751 pKa = 10.66 VFSVTEE757 pKa = 3.98 KK758 pKa = 9.28 TQLDD762 pKa = 3.68 SRR764 pKa = 11.84 DD765 pKa = 3.77 AEE767 pKa = 4.74 DD768 pKa = 3.58 ICDD771 pKa = 3.66 LRR773 pKa = 11.84 AFNLNQDD780 pKa = 3.93 DD781 pKa = 3.87 TDD783 pKa = 4.25 EE784 pKa = 5.69 IIISDD789 pKa = 4.26 CQWGEE794 pKa = 4.0 VQAFTLDD801 pKa = 3.65 EE802 pKa = 4.32 NSALAQLWSVDD813 pKa = 3.91 LQEE816 pKa = 5.23 HH817 pKa = 6.74 GSTSLSAGDD826 pKa = 4.12 IDD828 pKa = 4.26 NDD830 pKa = 3.59 GHH832 pKa = 8.37 IEE834 pKa = 4.08 VLWGSGVSSSGRR846 pKa = 11.84 DD847 pKa = 3.2 ILVTADD853 pKa = 3.55 VVGTTATVKK862 pKa = 10.72 PEE864 pKa = 4.0 TSPVQLDD871 pKa = 3.58 AFAAAGWATTGADD884 pKa = 3.58 DD885 pKa = 3.87 EE886 pKa = 4.66 QGVFIVPTTDD896 pKa = 2.54 SGYY899 pKa = 10.65 SGLSIARR906 pKa = 11.84 LNQNGTVNVNDD917 pKa = 4.18 KK918 pKa = 10.78 FSSFWQSGMFSAITDD933 pKa = 3.65 TDD935 pKa = 3.27 NDD937 pKa = 4.94 GIDD940 pKa = 5.52 DD941 pKa = 4.05 ILLPTTGTYY950 pKa = 10.23 DD951 pKa = 3.35 YY952 pKa = 11.36 GLGAFALSDD961 pKa = 4.18 LSSLWTVYY969 pKa = 10.97 GDD971 pKa = 3.43 YY972 pKa = 9.49 DD973 pKa = 4.13 TNINQIKK980 pKa = 9.88 LQDD983 pKa = 3.71 INNDD987 pKa = 2.6 GHH989 pKa = 6.21 TDD991 pKa = 3.18 VMFADD996 pKa = 4.34 GDD998 pKa = 4.05 VLTVIDD1004 pKa = 4.39 TQTQEE1009 pKa = 3.61 QLTSYY1014 pKa = 10.52 RR1015 pKa = 11.84 FSQSIADD1022 pKa = 4.11 FDD1024 pKa = 3.95 VADD1027 pKa = 3.78 IDD1029 pKa = 4.24 GVTTVLVTLRR1039 pKa = 11.84 EE1040 pKa = 4.03 QVHH1043 pKa = 6.16 ILTLKK1048 pKa = 7.09 EE1049 pKa = 3.89 TRR1051 pKa = 11.84 FTVVDD1056 pKa = 4.0 SVSDD1060 pKa = 3.39 ILCNRR1065 pKa = 11.84 ILFYY1069 pKa = 10.83 GQSNQRR1075 pKa = 11.84 SVACLWTDD1083 pKa = 3.17 YY1084 pKa = 10.81 GASLRR1089 pKa = 11.84 TMRR1092 pKa = 11.84 IVDD1095 pKa = 3.59 NQATEE1100 pKa = 4.39 LEE1102 pKa = 4.68 TFALEE1107 pKa = 4.13 HH1108 pKa = 6.1 NAVAIAVDD1116 pKa = 3.57 PTDD1119 pKa = 3.53 PQRR1122 pKa = 11.84 FIIAANTEE1130 pKa = 4.27 DD1131 pKa = 2.97 GWYY1134 pKa = 9.88 DD1135 pKa = 3.42 ARR1137 pKa = 11.84 SQLHH1141 pKa = 6.41 SLTAQGKK1148 pKa = 7.73 LVWSGPVLIGSANVASLQLRR1168 pKa = 11.84 EE1169 pKa = 4.06 QTSGKK1174 pKa = 10.6 LEE1176 pKa = 3.91 ILYY1179 pKa = 7.92 GTSKK1183 pKa = 10.6 GMYY1186 pKa = 8.4 WIHH1189 pKa = 6.63
Molecular weight: 129.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.77
IPC_protein 3.821
Toseland 3.592
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 1.456
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A346NH93|A0A346NH93_9ALTE Probable chromosome-partitioning protein ParB OS=Salinimonas sediminis OX=2303538 GN=D0Y50_00070 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3700
0
3700
1236480
11
1702
334.2
36.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.838 ± 0.047
1.005 ± 0.015
5.599 ± 0.038
5.609 ± 0.041
4.018 ± 0.024
6.89 ± 0.038
2.41 ± 0.023
5.817 ± 0.03
4.379 ± 0.032
10.259 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.023
3.993 ± 0.023
4.206 ± 0.026
5.03 ± 0.034
4.984 ± 0.028
6.27 ± 0.033
5.732 ± 0.027
6.989 ± 0.033
1.287 ± 0.017
3.159 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here