Acinetobacter phage vB_AbaM_IME285

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Obolenskvirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BXF0|A0A3G3BXF0_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME285 OX=2419626 PE=4 SV=1
MM1 pKa = 7.83IYY3 pKa = 9.77QVPLAQVPNQFFTTSLNGVTWSITLEE29 pKa = 3.98TRR31 pKa = 11.84LNNLYY36 pKa = 10.26ISLYY40 pKa = 10.78NNDD43 pKa = 4.74DD44 pKa = 3.37GDD46 pKa = 3.83VLLNRR51 pKa = 11.84ICLNRR56 pKa = 11.84TYY58 pKa = 10.23LGHH61 pKa = 6.75GFIFVDD67 pKa = 3.51IDD69 pKa = 3.79GNSDD73 pKa = 3.53PEE75 pKa = 4.25YY76 pKa = 9.62TGLGTRR82 pKa = 11.84YY83 pKa = 10.14LLIWTDD89 pKa = 3.71EE90 pKa = 4.13VV91 pKa = 3.51

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BXJ2|A0A3G3BXJ2_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME285 OX=2419626 PE=4 SV=1
MM1 pKa = 7.27NPIAMILLSFALSFVFVVVLYY22 pKa = 10.63LYY24 pKa = 9.64EE25 pKa = 4.25AYY27 pKa = 9.53KK28 pKa = 10.26RR29 pKa = 11.84KK30 pKa = 10.12RR31 pKa = 11.84YY32 pKa = 7.46VQRR35 pKa = 11.84KK36 pKa = 8.76QDD38 pKa = 3.67DD39 pKa = 3.52QRR41 pKa = 11.84KK42 pKa = 8.64FNPIRR47 pKa = 11.84EE48 pKa = 4.38KK49 pKa = 10.97VWWKK53 pKa = 10.77

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

13935

37

732

163.9

18.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.939 ± 0.386

1.227 ± 0.128

5.978 ± 0.261

6.566 ± 0.331

4.363 ± 0.221

7.277 ± 0.303

1.715 ± 0.155

7.104 ± 0.288

7.786 ± 0.351

8.08 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.135

5.684 ± 0.233

3.036 ± 0.182

3.861 ± 0.274

3.947 ± 0.217

6.38 ± 0.239

5.368 ± 0.317

6.408 ± 0.228

1.371 ± 0.127

3.997 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski