Togninia minima (strain UCR-PA7) (Esca disease fungus) (Phaeoacremonium aleophilum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Togniniales; Togniniaceae; Phaeoacremonium; Phaeoacremonium minimum

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8833 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R8BHP3|R8BHP3_TOGMI Putative nad dependent epimerase dehydratase protein OS=Togninia minima (strain UCR-PA7) OX=1286976 GN=UCRPA7_5681 PE=4 SV=1
MM1 pKa = 7.0RR2 pKa = 11.84TVAAVAALLALAGSAIAGTLKK23 pKa = 10.55IPVHH27 pKa = 5.47KK28 pKa = 10.05QGPGSAVSKK37 pKa = 10.84HH38 pKa = 5.62PLLTKK43 pKa = 9.93RR44 pKa = 11.84ASAIEE49 pKa = 3.86EE50 pKa = 4.19LVNNITGGGYY60 pKa = 8.88YY61 pKa = 10.59ASVSVGTPGQSINLVLDD78 pKa = 3.76TGSSDD83 pKa = 3.49AFVVDD88 pKa = 4.89VNADD92 pKa = 3.31LCTSRR97 pKa = 11.84RR98 pKa = 11.84LQVEE102 pKa = 3.9YY103 pKa = 11.2GMTCDD108 pKa = 3.48ATFDD112 pKa = 4.04SSDD115 pKa = 3.07SSTFSVVQQNGFSIEE130 pKa = 4.06YY131 pKa = 9.87LDD133 pKa = 4.65GSTASGDD140 pKa = 3.73FVSDD144 pKa = 3.15SFEE147 pKa = 4.15IADD150 pKa = 3.59VTLDD154 pKa = 3.67DD155 pKa = 4.42FQFGLADD162 pKa = 3.57STVTGTGVLGIGFVAEE178 pKa = 4.29EE179 pKa = 4.44VALTTYY185 pKa = 10.83PNLPQALVDD194 pKa = 4.11AGAISVNAYY203 pKa = 10.16SLYY206 pKa = 10.44LNEE209 pKa = 4.48YY210 pKa = 10.57DD211 pKa = 4.77SDD213 pKa = 3.69TGSILFGGVDD223 pKa = 3.39TEE225 pKa = 4.24KK226 pKa = 10.92FIGEE230 pKa = 4.23LTVLPILQDD239 pKa = 3.23ARR241 pKa = 11.84GRR243 pKa = 11.84NYY245 pKa = 10.73SSFTIGLAEE254 pKa = 4.08LAAVFSNGTDD264 pKa = 3.47PANITLPSVLPAILDD279 pKa = 3.67SGTTLSYY286 pKa = 11.11LPDD289 pKa = 4.36DD290 pKa = 3.75VATPLFEE297 pKa = 4.76AVNAYY302 pKa = 8.91TYY304 pKa = 10.71TEE306 pKa = 4.68GSQGLTLIDD315 pKa = 4.19CKK317 pKa = 11.24YY318 pKa = 10.57LDD320 pKa = 3.99SDD322 pKa = 4.77EE323 pKa = 4.41EE324 pKa = 4.17LTMNFGFASSLTSSSSEE341 pKa = 3.62VTISVRR347 pKa = 11.84AAEE350 pKa = 4.47LVLDD354 pKa = 4.56VYY356 pKa = 10.86QGYY359 pKa = 9.4QSQMPSEE366 pKa = 4.19IPFDD370 pKa = 4.09EE371 pKa = 4.41VCLFGLQSSGVALPSGQAQSIEE393 pKa = 4.04FALLGDD399 pKa = 3.87TFLRR403 pKa = 11.84SAYY406 pKa = 9.88VVYY409 pKa = 10.43HH410 pKa = 6.06LEE412 pKa = 3.78NRR414 pKa = 11.84QIGLAQANLGSSDD427 pKa = 3.83SDD429 pKa = 3.78VQEE432 pKa = 4.56LSASGTSLPVFTGVASQASNPTATTTRR459 pKa = 11.84SSGGSGNGGGSSSTSQSGMVIITVTASPTASNNAAVASTRR499 pKa = 11.84PPTGEE504 pKa = 3.9AFAVMAVVGIFTLFGGLLFALL525 pKa = 4.79

Molecular weight: 54.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R8BVY4|R8BVY4_TOGMI Ribosome biogenesis protein RLP24 OS=Togninia minima (strain UCR-PA7) OX=1286976 GN=UCRPA7_963 PE=3 SV=1
MM1 pKa = 6.93VGRR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 5.89HH7 pKa = 6.51HH8 pKa = 5.09TTTTTTTRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84GLFSSRR24 pKa = 11.84RR25 pKa = 11.84QPVVVHH31 pKa = 5.1HH32 pKa = 6.06QKK34 pKa = 10.13RR35 pKa = 11.84HH36 pKa = 4.31ATLGDD41 pKa = 4.03KK42 pKa = 10.67ISGAFLKK49 pKa = 10.97LKK51 pKa = 10.56GSLTGRR57 pKa = 11.84PGEE60 pKa = 4.13KK61 pKa = 9.9AAGTRR66 pKa = 11.84RR67 pKa = 11.84MRR69 pKa = 11.84GTDD72 pKa = 2.74GRR74 pKa = 11.84GSHH77 pKa = 5.71RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84FFF83 pKa = 4.33

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8833

0

8833

3911874

66

10679

442.9

48.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.1 ± 0.026

1.07 ± 0.008

6.047 ± 0.02

6.422 ± 0.03

3.849 ± 0.017

7.343 ± 0.025

2.138 ± 0.011

5.117 ± 0.017

5.337 ± 0.025

8.707 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.011

3.676 ± 0.014

5.489 ± 0.024

3.793 ± 0.017

5.581 ± 0.02

7.316 ± 0.023

5.857 ± 0.02

6.508 ± 0.017

1.491 ± 0.011

2.896 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski