Gardnerella vaginalis 00703Dmash

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Gardnerella; Gardnerella vaginalis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4MBK1|I4MBK1_GARVA DNA-damage-inducible protein P OS=Gardnerella vaginalis 00703Dmash OX=698960 GN=CGSMWGv00703Dmash_01755 PE=3 SV=1
MM1 pKa = 7.9PYY3 pKa = 10.33VIAEE7 pKa = 4.09PCVDD11 pKa = 4.1VKK13 pKa = 11.38DD14 pKa = 3.94KK15 pKa = 11.56ACVDD19 pKa = 4.0EE20 pKa = 5.6CPVDD24 pKa = 4.37CIYY27 pKa = 11.15EE28 pKa = 4.18GDD30 pKa = 3.46RR31 pKa = 11.84TLYY34 pKa = 10.34INPNEE39 pKa = 4.32CVDD42 pKa = 4.51CGACEE47 pKa = 3.78PACPVEE53 pKa = 4.47AIFYY57 pKa = 10.59EE58 pKa = 4.77DD59 pKa = 4.85DD60 pKa = 4.73LPEE63 pKa = 4.0GWEE66 pKa = 4.07WYY68 pKa = 10.03RR69 pKa = 11.84DD70 pKa = 3.43AAVDD74 pKa = 3.88YY75 pKa = 10.47FDD77 pKa = 5.63KK78 pKa = 11.47LGDD81 pKa = 3.74LGGATDD87 pKa = 4.59AGASGWDD94 pKa = 3.72EE95 pKa = 4.0EE96 pKa = 4.78RR97 pKa = 11.84VAALPSRR104 pKa = 11.84ADD106 pKa = 3.18KK107 pKa = 11.18AGNN110 pKa = 3.3

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4MB21|I4MB21_GARVA tRNA nucleotidyltransferase OS=Gardnerella vaginalis 00703Dmash OX=698960 GN=CGSMWGv00703Dmash_00845 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.83MKK15 pKa = 9.27HH16 pKa = 5.56GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1166

0

1166

436347

31

3198

374.2

41.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.893 ± 0.087

1.062 ± 0.031

6.024 ± 0.067

6.031 ± 0.068

3.543 ± 0.043

6.816 ± 0.056

2.06 ± 0.025

6.023 ± 0.065

5.805 ± 0.092

8.77 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.037

4.792 ± 0.074

4.043 ± 0.043

3.525 ± 0.042

5.063 ± 0.062

7.126 ± 0.061

5.511 ± 0.063

7.48 ± 0.065

1.164 ± 0.029

2.833 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski