Cognatiyoonia sediminum
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3230 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5QR44|A0A1M5QR44_9RHOB HemY protein OS=Cognatiyoonia sediminum OX=1508389 GN=SAMN05444003_2262 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 4.69 KK3 pKa = 10.63 KK4 pKa = 10.62 IAIISATALGALLLGSVAFILRR26 pKa = 11.84 GSAYY30 pKa = 10.37 ASACSASVVAGGAIGGPFTLVDD52 pKa = 3.69 EE53 pKa = 5.01 SGVEE57 pKa = 4.07 VTDD60 pKa = 3.64 QDD62 pKa = 4.82 VIDD65 pKa = 4.98 APSLIYY71 pKa = 10.29 FGYY74 pKa = 8.28 TFCPDD79 pKa = 4.22 FCPLDD84 pKa = 3.67 AQRR87 pKa = 11.84 NAVTSDD93 pKa = 2.58 ILADD97 pKa = 3.51 QGLDD101 pKa = 3.34 LNTVFITIDD110 pKa = 3.48 PARR113 pKa = 11.84 DD114 pKa = 3.56 TPEE117 pKa = 3.66 VLAEE121 pKa = 4.09 YY122 pKa = 9.94 TDD124 pKa = 3.83 NFHH127 pKa = 7.39 PDD129 pKa = 3.19 MVGLTGSDD137 pKa = 3.58 EE138 pKa = 4.34 QIKK141 pKa = 10.27 AAANEE146 pKa = 4.12 YY147 pKa = 10.04 KK148 pKa = 10.46 VVYY151 pKa = 10.34 SRR153 pKa = 11.84 ADD155 pKa = 3.69 DD156 pKa = 4.51 DD157 pKa = 4.5 PEE159 pKa = 5.19 FYY161 pKa = 11.25 LMNHH165 pKa = 5.57 SVFSYY170 pKa = 10.06 FVTPEE175 pKa = 3.93 DD176 pKa = 3.76 GFVDD180 pKa = 4.59 FFKK183 pKa = 11.32 NDD185 pKa = 3.61 DD186 pKa = 3.68 TPEE189 pKa = 3.94 AVAEE193 pKa = 4.78 RR194 pKa = 11.84 IACHH198 pKa = 6.29 IGG200 pKa = 3.0
Molecular weight: 21.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.63
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.138
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A1M5SVN0|A0A1M5SVN0_9RHOB L-glutamine-binding protein /L-glutamate-binding protein /L-aspartate-binding protein /L-asparagine-binding protein OS=Cognatiyoonia sediminum OX=1508389 GN=SAMN05444003_3147 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.35 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3230
0
3230
985169
26
3813
305.0
33.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.09 ± 0.056
0.914 ± 0.012
6.401 ± 0.04
6.286 ± 0.042
4.011 ± 0.032
8.399 ± 0.034
1.976 ± 0.021
5.685 ± 0.033
3.716 ± 0.039
9.578 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.826 ± 0.023
3.053 ± 0.022
4.727 ± 0.029
3.273 ± 0.023
5.778 ± 0.039
5.579 ± 0.032
5.646 ± 0.031
7.304 ± 0.035
1.398 ± 0.02
2.359 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here