Poseidonibacter parvus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2691 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8KNE5|A0A1P8KNE5_9PROT Uncharacterized protein OS=Poseidonibacter parvus OX=1850254 GN=LPB137_09530 PE=3 SV=1
MM1 pKa = 7.66 NDD3 pKa = 3.23 YY4 pKa = 10.86 LCTVRR9 pKa = 11.84 GYY11 pKa = 10.3 IYY13 pKa = 10.03 QPKK16 pKa = 10.43 LGDD19 pKa = 3.75 PDD21 pKa = 4.57 VGIKK25 pKa = 10.27 PGTAFEE31 pKa = 4.5 EE32 pKa = 4.6 LPEE35 pKa = 4.5 DD36 pKa = 4.45 WEE38 pKa = 4.94 CPDD41 pKa = 4.24 CGSPKK46 pKa = 10.47 EE47 pKa = 4.18 DD48 pKa = 3.55 FEE50 pKa = 4.58 EE51 pKa = 4.34 LLEE54 pKa = 4.22 EE55 pKa = 4.32
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.859
IPC_protein 3.745
Toseland 3.567
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.478
Solomon 3.694
Lehninger 3.643
Nozaki 3.846
DTASelect 3.973
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A1P8KP38|A0A1P8KP38_9PROT Uncharacterized protein OS=Poseidonibacter parvus OX=1850254 GN=LPB137_10880 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 6.0 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MATKK25 pKa = 10.2 NGRR28 pKa = 11.84 KK29 pKa = 9.2 VIKK32 pKa = 10.14 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2691
0
2691
878469
24
11219
326.4
36.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.896 ± 0.058
0.845 ± 0.02
6.064 ± 0.14
7.031 ± 0.059
5.047 ± 0.064
5.449 ± 0.131
1.513 ± 0.03
9.425 ± 0.06
9.308 ± 0.133
9.628 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.042
6.474 ± 0.064
2.651 ± 0.043
2.799 ± 0.032
2.738 ± 0.043
6.866 ± 0.052
5.428 ± 0.124
5.901 ± 0.065
0.713 ± 0.016
3.941 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here