Devosia crocina
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3590 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I7ND35|A0A1I7ND35_9RHIZ NADH-quinone oxidoreductase subunit N OS=Devosia crocina OX=429728 GN=nuoN PE=3 SV=1
MM1 pKa = 7.34 FVRR4 pKa = 11.84 SLSLGVAAAALMVGGAQAADD24 pKa = 4.78 LIIPTTPQPIYY35 pKa = 10.56 EE36 pKa = 4.27 SAGFDD41 pKa = 3.62 WEE43 pKa = 4.15 GLYY46 pKa = 10.9 AGVRR50 pKa = 11.84 IGGQWVGEE58 pKa = 3.88 QSYY61 pKa = 11.4 GNIGFGDD68 pKa = 5.52 DD69 pKa = 3.08 ITQGVIGGAVGVNFIPVDD87 pKa = 3.64 PFLLGLEE94 pKa = 4.28 VTGDD98 pKa = 4.04 YY99 pKa = 11.2 VWGNDD104 pKa = 3.18 LATGEE109 pKa = 4.31 FFANLRR115 pKa = 11.84 AGAVVTDD122 pKa = 3.89 SVLVYY127 pKa = 10.78 ALGGVGVQTGPEE139 pKa = 4.38 GTSGTEE145 pKa = 3.61 GLYY148 pKa = 10.24 QLGGGVEE155 pKa = 4.3 FAVTDD160 pKa = 5.21 AITVRR165 pKa = 11.84 GEE167 pKa = 3.73 IVGQGDD173 pKa = 3.54 FADD176 pKa = 4.01 NGNDD180 pKa = 3.56 FFNTAKK186 pKa = 9.86 ATVGVFYY193 pKa = 10.88 HH194 pKa = 6.45 FF195 pKa = 4.46
Molecular weight: 20.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.808
Patrickios 0.846
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A1I7NNJ9|A0A1I7NNJ9_9RHIZ Regulator of nucleoside diphosphate kinase OS=Devosia crocina OX=429728 GN=SAMN05216456_2390 PE=4 SV=1
MM1 pKa = 7.43 LRR3 pKa = 11.84 QRR5 pKa = 11.84 TLSSQPHH12 pKa = 5.43 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 HH18 pKa = 5.25 HH19 pKa = 7.02 RR20 pKa = 11.84 IFMSAAVSPIEE31 pKa = 3.91 SRR33 pKa = 11.84 ANLGIALVLTSQIVLLMLDD52 pKa = 4.79 ISAKK56 pKa = 7.63 WLSVEE61 pKa = 4.36 GMPTTEE67 pKa = 3.71 IVFVRR72 pKa = 11.84 YY73 pKa = 9.66 GMHH76 pKa = 7.44 LALLLLLFLPVSGARR91 pKa = 11.84 LFQTGNLKK99 pKa = 10.91 LEE101 pKa = 4.35 ILRR104 pKa = 11.84 GACLLITTGLNFLAMKK120 pKa = 8.72 FLPLTVTAAIQFTSPLIICALSGPLLGDD148 pKa = 3.3 KK149 pKa = 10.66 VGWRR153 pKa = 11.84 RR154 pKa = 11.84 WLAIGCGFVGILIIVRR170 pKa = 11.84 PGSEE174 pKa = 3.97 AFQPAAFLSLACAFFLALFSILTRR198 pKa = 11.84 KK199 pKa = 9.27 LAGVDD204 pKa = 3.56 SAQTQQFFAGATPVILLLPVAFTDD228 pKa = 4.02 WVWPSQPISWVAFFVMGAAGLGGHH252 pKa = 6.62 FLNSVAHH259 pKa = 6.9 RR260 pKa = 11.84 FATPATLAPFSYY272 pKa = 10.68 LSLVYY277 pKa = 10.27 LSIASWLIFNEE288 pKa = 4.78 PPDD291 pKa = 3.31 EE292 pKa = 4.07 WFILGVGIIISSGLYY307 pKa = 7.67 IWLRR311 pKa = 11.84 EE312 pKa = 4.09 RR313 pKa = 11.84 QLSRR317 pKa = 11.84 PVTGEE322 pKa = 3.66 VNLRR326 pKa = 3.52
Molecular weight: 35.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.545
IPC_protein 10.511
Toseland 10.54
ProMoST 10.584
Dawson 10.657
Bjellqvist 10.438
Wikipedia 10.906
Rodwell 10.73
Grimsley 10.73
Solomon 10.804
Lehninger 10.76
Nozaki 10.57
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.438
IPC_peptide 10.804
IPC2_peptide 9.736
IPC2.peptide.svr19 8.676
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3590
0
3590
1108822
32
2668
308.9
33.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.208 ± 0.056
0.666 ± 0.012
5.7 ± 0.035
5.882 ± 0.04
3.865 ± 0.03
8.511 ± 0.038
2.008 ± 0.02
5.443 ± 0.03
3.021 ± 0.03
10.287 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.018
2.774 ± 0.023
5.034 ± 0.03
3.32 ± 0.025
6.619 ± 0.039
5.591 ± 0.029
5.467 ± 0.025
7.537 ± 0.037
1.305 ± 0.018
2.23 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here