Amino acid dipepetide frequency for Gordonia phage Guacamole

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.033AlaAla: 17.033 ± 1.311
0.617AlaCys: 0.617 ± 0.176
8.331AlaAsp: 8.331 ± 0.758
6.665AlaGlu: 6.665 ± 0.687
3.333AlaPhe: 3.333 ± 0.545
9.195AlaGly: 9.195 ± 0.978
2.037AlaHis: 2.037 ± 0.362
5.554AlaIle: 5.554 ± 0.551
3.641AlaLys: 3.641 ± 0.649
10.615AlaLeu: 10.615 ± 1.086
2.962AlaMet: 2.962 ± 0.583
3.209AlaAsn: 3.209 ± 0.485
5.739AlaPro: 5.739 ± 0.599
4.258AlaGln: 4.258 ± 0.535
8.455AlaArg: 8.455 ± 0.825
6.233AlaSer: 6.233 ± 0.768
6.912AlaThr: 6.912 ± 0.583
7.776AlaVal: 7.776 ± 0.866
2.037AlaTrp: 2.037 ± 0.292
1.913AlaTyr: 1.913 ± 0.317
0.0AlaXaa: 0.0 ± 0.0
Cys
0.679CysAla: 0.679 ± 0.222
0.123CysCys: 0.123 ± 0.113
1.111CysAsp: 1.111 ± 0.349
0.247CysGlu: 0.247 ± 0.125
0.062CysPhe: 0.062 ± 0.064
0.741CysGly: 0.741 ± 0.24
0.555CysHis: 0.555 ± 0.247
0.123CysIle: 0.123 ± 0.081
0.123CysLys: 0.123 ± 0.089
0.494CysLeu: 0.494 ± 0.151
0.432CysMet: 0.432 ± 0.169
0.247CysAsn: 0.247 ± 0.134
0.926CysPro: 0.926 ± 0.259
0.37CysGln: 0.37 ± 0.146
0.432CysArg: 0.432 ± 0.155
0.309CysSer: 0.309 ± 0.137
0.555CysThr: 0.555 ± 0.199
0.37CysVal: 0.37 ± 0.155
0.185CysTrp: 0.185 ± 0.155
0.123CysTyr: 0.123 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
7.035AspAla: 7.035 ± 0.631
0.37AspCys: 0.37 ± 0.158
5.307AspAsp: 5.307 ± 0.697
4.32AspGlu: 4.32 ± 0.491
1.913AspPhe: 1.913 ± 0.346
7.035AspGly: 7.035 ± 0.837
1.913AspHis: 1.913 ± 0.367
2.901AspIle: 2.901 ± 0.406
1.481AspLys: 1.481 ± 0.322
6.171AspLeu: 6.171 ± 0.732
1.481AspMet: 1.481 ± 0.334
1.975AspAsn: 1.975 ± 0.35
3.888AspPro: 3.888 ± 0.528
2.777AspGln: 2.777 ± 0.365
4.443AspArg: 4.443 ± 0.697
3.579AspSer: 3.579 ± 0.406
4.073AspThr: 4.073 ± 0.51
5.801AspVal: 5.801 ± 0.683
1.234AspTrp: 1.234 ± 0.224
1.851AspTyr: 1.851 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
5.554GluAla: 5.554 ± 0.632
0.555GluCys: 0.555 ± 0.182
3.086GluAsp: 3.086 ± 0.411
2.16GluGlu: 2.16 ± 0.444
2.098GluPhe: 2.098 ± 0.27
4.196GluGly: 4.196 ± 0.531
1.173GluHis: 1.173 ± 0.281
2.469GluIle: 2.469 ± 0.344
1.975GluLys: 1.975 ± 0.306
4.814GluLeu: 4.814 ± 0.647
1.666GluMet: 1.666 ± 0.316
1.173GluAsn: 1.173 ± 0.26
3.765GluPro: 3.765 ± 0.653
2.962GluGln: 2.962 ± 0.485
4.505GluArg: 4.505 ± 0.576
2.037GluSer: 2.037 ± 0.328
2.53GluThr: 2.53 ± 0.352
5.184GluVal: 5.184 ± 0.466
1.173GluTrp: 1.173 ± 0.276
1.605GluTyr: 1.605 ± 0.368
0.0GluXaa: 0.0 ± 0.0
Phe
3.147PheAla: 3.147 ± 0.437
0.123PheCys: 0.123 ± 0.077
2.283PheAsp: 2.283 ± 0.31
1.913PheGlu: 1.913 ± 0.31
0.926PhePhe: 0.926 ± 0.21
2.16PheGly: 2.16 ± 0.353
0.432PheHis: 0.432 ± 0.174
0.864PheIle: 0.864 ± 0.256
1.049PheLys: 1.049 ± 0.275
1.913PheLeu: 1.913 ± 0.346
0.494PheMet: 0.494 ± 0.145
0.617PheAsn: 0.617 ± 0.195
1.605PhePro: 1.605 ± 0.251
0.617PheGln: 0.617 ± 0.165
2.345PheArg: 2.345 ± 0.28
1.666PheSer: 1.666 ± 0.406
2.16PheThr: 2.16 ± 0.282
2.715PheVal: 2.715 ± 0.37
0.432PheTrp: 0.432 ± 0.173
0.802PheTyr: 0.802 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
8.578GlyAla: 8.578 ± 1.013
0.494GlyCys: 0.494 ± 0.204
5.369GlyAsp: 5.369 ± 0.495
4.752GlyGlu: 4.752 ± 0.677
2.777GlyPhe: 2.777 ± 0.482
6.727GlyGly: 6.727 ± 0.839
1.79GlyHis: 1.79 ± 0.319
3.95GlyIle: 3.95 ± 0.538
3.518GlyLys: 3.518 ± 0.503
8.146GlyLeu: 8.146 ± 1.083
1.358GlyMet: 1.358 ± 0.243
2.469GlyAsn: 2.469 ± 0.386
3.703GlyPro: 3.703 ± 0.487
3.209GlyGln: 3.209 ± 0.377
6.788GlyArg: 6.788 ± 0.581
4.814GlySer: 4.814 ± 0.588
4.567GlyThr: 4.567 ± 0.495
5.924GlyVal: 5.924 ± 0.696
2.283GlyTrp: 2.283 ± 0.382
2.53GlyTyr: 2.53 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
2.592HisAla: 2.592 ± 0.334
0.247HisCys: 0.247 ± 0.134
1.79HisAsp: 1.79 ± 0.298
0.741HisGlu: 0.741 ± 0.163
0.555HisPhe: 0.555 ± 0.201
1.419HisGly: 1.419 ± 0.307
0.494HisHis: 0.494 ± 0.199
0.987HisIle: 0.987 ± 0.262
0.494HisLys: 0.494 ± 0.179
1.975HisLeu: 1.975 ± 0.486
0.309HisMet: 0.309 ± 0.136
0.555HisAsn: 0.555 ± 0.159
1.605HisPro: 1.605 ± 0.274
0.679HisGln: 0.679 ± 0.21
1.79HisArg: 1.79 ± 0.267
1.049HisSer: 1.049 ± 0.274
1.111HisThr: 1.111 ± 0.289
1.543HisVal: 1.543 ± 0.318
0.432HisTrp: 0.432 ± 0.168
0.37HisTyr: 0.37 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
5.863IleAla: 5.863 ± 0.535
0.185IleCys: 0.185 ± 0.104
4.196IleAsp: 4.196 ± 0.541
2.345IleGlu: 2.345 ± 0.447
0.926IlePhe: 0.926 ± 0.215
4.999IleGly: 4.999 ± 0.77
0.679IleHis: 0.679 ± 0.189
1.419IleIle: 1.419 ± 0.35
1.728IleLys: 1.728 ± 0.384
2.715IleLeu: 2.715 ± 0.5
0.309IleMet: 0.309 ± 0.125
0.987IleAsn: 0.987 ± 0.248
3.271IlePro: 3.271 ± 0.471
0.926IleGln: 0.926 ± 0.219
3.579IleArg: 3.579 ± 0.491
2.098IleSer: 2.098 ± 0.408
3.703IleThr: 3.703 ± 0.409
4.258IleVal: 4.258 ± 0.428
0.432IleTrp: 0.432 ± 0.125
0.926IleTyr: 0.926 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
3.394LysAla: 3.394 ± 0.481
0.37LysCys: 0.37 ± 0.168
1.79LysAsp: 1.79 ± 0.435
1.234LysGlu: 1.234 ± 0.278
1.234LysPhe: 1.234 ± 0.287
2.345LysGly: 2.345 ± 0.422
0.555LysHis: 0.555 ± 0.245
1.481LysIle: 1.481 ± 0.358
1.975LysLys: 1.975 ± 0.363
2.901LysLeu: 2.901 ± 0.426
0.802LysMet: 0.802 ± 0.274
0.864LysAsn: 0.864 ± 0.271
2.715LysPro: 2.715 ± 0.382
1.049LysGln: 1.049 ± 0.267
2.222LysArg: 2.222 ± 0.319
1.851LysSer: 1.851 ± 0.349
2.283LysThr: 2.283 ± 0.415
2.715LysVal: 2.715 ± 0.396
0.617LysTrp: 0.617 ± 0.185
0.741LysTyr: 0.741 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
11.664LeuAla: 11.664 ± 0.841
0.679LeuCys: 0.679 ± 0.205
5.739LeuAsp: 5.739 ± 0.678
3.456LeuGlu: 3.456 ± 0.545
2.098LeuPhe: 2.098 ± 0.348
6.542LeuGly: 6.542 ± 0.713
1.049LeuHis: 1.049 ± 0.237
3.456LeuIle: 3.456 ± 0.388
2.037LeuLys: 2.037 ± 0.295
5.122LeuLeu: 5.122 ± 0.687
2.098LeuMet: 2.098 ± 0.39
2.283LeuAsn: 2.283 ± 0.39
4.628LeuPro: 4.628 ± 0.491
1.79LeuGln: 1.79 ± 0.321
4.999LeuArg: 4.999 ± 0.709
5.122LeuSer: 5.122 ± 0.617
5.863LeuThr: 5.863 ± 0.668
6.233LeuVal: 6.233 ± 0.643
2.345LeuTrp: 2.345 ± 0.394
1.543LeuTyr: 1.543 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
3.518MetAla: 3.518 ± 0.483
0.185MetCys: 0.185 ± 0.115
0.741MetAsp: 0.741 ± 0.193
0.741MetGlu: 0.741 ± 0.197
0.741MetPhe: 0.741 ± 0.191
1.605MetGly: 1.605 ± 0.315
0.247MetHis: 0.247 ± 0.119
0.926MetIle: 0.926 ± 0.22
0.741MetLys: 0.741 ± 0.204
1.543MetLeu: 1.543 ± 0.319
0.185MetMet: 0.185 ± 0.143
0.617MetAsn: 0.617 ± 0.202
1.913MetPro: 1.913 ± 0.344
0.802MetGln: 0.802 ± 0.273
2.222MetArg: 2.222 ± 0.434
1.728MetSer: 1.728 ± 0.347
2.469MetThr: 2.469 ± 0.444
0.741MetVal: 0.741 ± 0.237
0.679MetTrp: 0.679 ± 0.225
0.309MetTyr: 0.309 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
2.283AsnAla: 2.283 ± 0.454
0.247AsnCys: 0.247 ± 0.138
1.913AsnAsp: 1.913 ± 0.283
0.926AsnGlu: 0.926 ± 0.187
0.555AsnPhe: 0.555 ± 0.16
3.271AsnGly: 3.271 ± 0.454
0.741AsnHis: 0.741 ± 0.23
0.741AsnIle: 0.741 ± 0.221
0.679AsnLys: 0.679 ± 0.199
2.283AsnLeu: 2.283 ± 0.325
0.617AsnMet: 0.617 ± 0.179
0.679AsnAsn: 0.679 ± 0.229
2.901AsnPro: 2.901 ± 0.41
0.926AsnGln: 0.926 ± 0.234
1.913AsnArg: 1.913 ± 0.437
1.851AsnSer: 1.851 ± 0.316
2.407AsnThr: 2.407 ± 0.404
2.098AsnVal: 2.098 ± 0.383
0.494AsnTrp: 0.494 ± 0.159
0.802AsnTyr: 0.802 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
6.295ProAla: 6.295 ± 0.8
0.802ProCys: 0.802 ± 0.282
4.443ProAsp: 4.443 ± 0.501
4.505ProGlu: 4.505 ± 0.608
1.728ProPhe: 1.728 ± 0.327
5.307ProGly: 5.307 ± 0.641
1.234ProHis: 1.234 ± 0.246
3.271ProIle: 3.271 ± 0.409
2.592ProLys: 2.592 ± 0.345
3.333ProLeu: 3.333 ± 0.556
1.358ProMet: 1.358 ± 0.402
1.79ProAsn: 1.79 ± 0.317
3.147ProPro: 3.147 ± 0.628
1.851ProGln: 1.851 ± 0.326
3.394ProArg: 3.394 ± 0.521
3.086ProSer: 3.086 ± 0.356
4.258ProThr: 4.258 ± 0.554
4.258ProVal: 4.258 ± 0.531
1.173ProTrp: 1.173 ± 0.263
1.358ProTyr: 1.358 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
3.209GlnAla: 3.209 ± 0.457
0.185GlnCys: 0.185 ± 0.104
1.173GlnAsp: 1.173 ± 0.289
1.419GlnGlu: 1.419 ± 0.287
1.173GlnPhe: 1.173 ± 0.358
2.345GlnGly: 2.345 ± 0.42
1.296GlnHis: 1.296 ± 0.288
1.358GlnIle: 1.358 ± 0.252
0.987GlnLys: 0.987 ± 0.191
3.456GlnLeu: 3.456 ± 0.374
0.987GlnMet: 0.987 ± 0.253
0.864GlnAsn: 0.864 ± 0.217
2.53GlnPro: 2.53 ± 0.466
1.851GlnGln: 1.851 ± 0.364
2.715GlnArg: 2.715 ± 0.427
1.913GlnSer: 1.913 ± 0.329
1.913GlnThr: 1.913 ± 0.365
2.592GlnVal: 2.592 ± 0.474
0.926GlnTrp: 0.926 ± 0.306
0.926GlnTyr: 0.926 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
8.084ArgAla: 8.084 ± 0.775
0.802ArgCys: 0.802 ± 0.264
5.739ArgAsp: 5.739 ± 0.588
4.258ArgGlu: 4.258 ± 0.486
1.975ArgPhe: 1.975 ± 0.357
5.924ArgGly: 5.924 ± 0.598
2.037ArgHis: 2.037 ± 0.444
3.826ArgIle: 3.826 ± 0.516
2.16ArgLys: 2.16 ± 0.311
5.616ArgLeu: 5.616 ± 0.506
2.098ArgMet: 2.098 ± 0.313
2.222ArgAsn: 2.222 ± 0.317
3.271ArgPro: 3.271 ± 0.443
2.715ArgGln: 2.715 ± 0.5
7.159ArgArg: 7.159 ± 1.034
3.765ArgSer: 3.765 ± 0.469
4.628ArgThr: 4.628 ± 0.553
5.369ArgVal: 5.369 ± 0.65
1.605ArgTrp: 1.605 ± 0.341
1.358ArgTyr: 1.358 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
6.418SerAla: 6.418 ± 0.79
0.123SerCys: 0.123 ± 0.093
3.086SerAsp: 3.086 ± 0.383
3.394SerGlu: 3.394 ± 0.591
1.481SerPhe: 1.481 ± 0.315
5.492SerGly: 5.492 ± 0.696
0.555SerHis: 0.555 ± 0.169
3.024SerIle: 3.024 ± 0.416
1.358SerLys: 1.358 ± 0.25
3.271SerLeu: 3.271 ± 0.496
1.419SerMet: 1.419 ± 0.251
2.037SerAsn: 2.037 ± 0.363
2.839SerPro: 2.839 ± 0.392
1.358SerGln: 1.358 ± 0.342
3.826SerArg: 3.826 ± 0.642
2.037SerSer: 2.037 ± 0.381
4.011SerThr: 4.011 ± 0.478
4.258SerVal: 4.258 ± 0.469
1.358SerTrp: 1.358 ± 0.232
0.926SerTyr: 0.926 ± 0.205
0.0SerXaa: 0.0 ± 0.0
Thr
7.591ThrAla: 7.591 ± 0.686
0.617ThrCys: 0.617 ± 0.228
4.999ThrAsp: 4.999 ± 0.608
3.765ThrGlu: 3.765 ± 0.527
2.16ThrPhe: 2.16 ± 0.457
5.492ThrGly: 5.492 ± 0.64
1.481ThrHis: 1.481 ± 0.327
3.456ThrIle: 3.456 ± 0.437
2.407ThrLys: 2.407 ± 0.439
5.307ThrLeu: 5.307 ± 0.579
0.987ThrMet: 0.987 ± 0.232
2.222ThrAsn: 2.222 ± 0.323
4.32ThrPro: 4.32 ± 0.659
2.16ThrGln: 2.16 ± 0.359
4.073ThrArg: 4.073 ± 0.521
3.147ThrSer: 3.147 ± 0.42
4.073ThrThr: 4.073 ± 0.469
6.233ThrVal: 6.233 ± 0.505
1.419ThrTrp: 1.419 ± 0.237
1.234ThrTyr: 1.234 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
9.319ValAla: 9.319 ± 0.964
1.111ValCys: 1.111 ± 0.324
6.233ValAsp: 6.233 ± 0.753
4.999ValGlu: 4.999 ± 0.606
1.419ValPhe: 1.419 ± 0.309
5.801ValGly: 5.801 ± 0.553
1.234ValHis: 1.234 ± 0.286
4.073ValIle: 4.073 ± 0.441
2.469ValLys: 2.469 ± 0.371
5.184ValLeu: 5.184 ± 0.655
1.975ValMet: 1.975 ± 0.321
1.79ValAsn: 1.79 ± 0.277
3.95ValPro: 3.95 ± 0.546
2.345ValGln: 2.345 ± 0.37
6.295ValArg: 6.295 ± 0.625
3.518ValSer: 3.518 ± 0.542
6.295ValThr: 6.295 ± 0.731
7.406ValVal: 7.406 ± 0.621
1.543ValTrp: 1.543 ± 0.367
1.975ValTyr: 1.975 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
2.098TrpAla: 2.098 ± 0.389
0.309TrpCys: 0.309 ± 0.144
1.296TrpAsp: 1.296 ± 0.336
0.802TrpGlu: 0.802 ± 0.211
0.679TrpPhe: 0.679 ± 0.25
0.864TrpGly: 0.864 ± 0.248
0.494TrpHis: 0.494 ± 0.165
0.802TrpIle: 0.802 ± 0.209
0.864TrpLys: 0.864 ± 0.209
2.222TrpLeu: 2.222 ± 0.4
0.494TrpMet: 0.494 ± 0.17
0.926TrpAsn: 0.926 ± 0.326
1.419TrpPro: 1.419 ± 0.273
0.555TrpGln: 0.555 ± 0.187
1.728TrpArg: 1.728 ± 0.307
1.296TrpSer: 1.296 ± 0.275
1.419TrpThr: 1.419 ± 0.217
1.79TrpVal: 1.79 ± 0.274
0.494TrpTrp: 0.494 ± 0.155
0.741TrpTyr: 0.741 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.16TyrAla: 2.16 ± 0.295
0.062TyrCys: 0.062 ± 0.063
0.926TyrAsp: 0.926 ± 0.209
2.037TyrGlu: 2.037 ± 0.38
0.432TyrPhe: 0.432 ± 0.157
2.283TyrGly: 2.283 ± 0.343
0.741TyrHis: 0.741 ± 0.197
0.864TyrIle: 0.864 ± 0.298
0.864TyrLys: 0.864 ± 0.245
1.666TyrLeu: 1.666 ± 0.33
0.555TyrMet: 0.555 ± 0.182
0.802TyrAsn: 0.802 ± 0.216
1.111TyrPro: 1.111 ± 0.232
0.617TyrGln: 0.617 ± 0.227
1.728TyrArg: 1.728 ± 0.3
1.173TyrSer: 1.173 ± 0.214
1.913TyrThr: 1.913 ± 0.329
1.666TyrVal: 1.666 ± 0.303
0.432TyrTrp: 0.432 ± 0.142
0.617TyrTyr: 0.617 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (16205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski