Bifidobacterium hapali

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261FYD3|A0A261FYD3_9BIFI Methylmalonyl-CoA carboxyltransferase OS=Bifidobacterium hapali OX=1630172 GN=BHAP_1619 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.82SPRR9 pKa = 11.84NKK11 pKa = 10.84DD12 pKa = 3.11EE13 pKa = 5.7DD14 pKa = 4.16EE15 pKa = 4.93EE16 pKa = 4.45SLQALGKK23 pKa = 10.12SSQNTSSDD31 pKa = 3.0IDD33 pKa = 3.89DD34 pKa = 4.97DD35 pKa = 4.45EE36 pKa = 4.37NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 5.03LSNEE53 pKa = 4.1DD54 pKa = 3.77ASVTVIPMQGDD65 pKa = 3.48EE66 pKa = 5.3FICSEE71 pKa = 4.5CYY73 pKa = 9.63LVKK76 pKa = 10.53HH77 pKa = 6.5RR78 pKa = 11.84SQLAYY83 pKa = 7.63TTEE86 pKa = 4.78DD87 pKa = 3.41GQPVCKK93 pKa = 10.23EE94 pKa = 3.94CAAA97 pKa = 4.21

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261G5C9|A0A261G5C9_9BIFI 50S ribosomal protein L27 OS=Bifidobacterium hapali OX=1630172 GN=rpmA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2249

0

2249

807543

29

3209

359.1

39.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.87 ± 0.071

0.969 ± 0.017

6.753 ± 0.05

5.224 ± 0.05

3.433 ± 0.036

7.605 ± 0.042

2.365 ± 0.026

5.624 ± 0.038

3.516 ± 0.047

8.756 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.027

3.535 ± 0.037

4.528 ± 0.029

3.502 ± 0.032

6.098 ± 0.053

6.203 ± 0.043

6.538 ± 0.055

7.633 ± 0.049

1.427 ± 0.019

2.844 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski