Escherichia phage phiv142-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; unclassified Uetakevirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2EZU9|A0A5Q2EZU9_9CAUD Uncharacterized protein OS=Escherichia phage phiv142-3 OX=2601647 PE=4 SV=1
MM1 pKa = 7.91SIRR4 pKa = 11.84TEE6 pKa = 4.01HH7 pKa = 6.52GFGPSTVEE15 pKa = 4.57VEE17 pKa = 4.22WLDD20 pKa = 3.66DD21 pKa = 4.3CPRR24 pKa = 11.84CQHH27 pKa = 5.1GKK29 pKa = 10.37ARR31 pKa = 11.84VTGWSVTKK39 pKa = 10.64DD40 pKa = 3.51SLWAGDD46 pKa = 3.55EE47 pKa = 4.35AVCSKK52 pKa = 10.4CGHH55 pKa = 6.53KK56 pKa = 11.02GEE58 pKa = 4.64IDD60 pKa = 3.53ADD62 pKa = 4.23GEE64 pKa = 4.46NAWVEE69 pKa = 3.49WDD71 pKa = 4.32EE72 pKa = 4.94IEE74 pKa = 4.42EE75 pKa = 4.19AQQ77 pKa = 4.76

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F2S2|A0A5Q2F2S2_9CAUD Uncharacterized protein OS=Escherichia phage phiv142-3 OX=2601647 PE=4 SV=1
MM1 pKa = 6.2VTHH4 pKa = 7.05EE5 pKa = 4.71FFLLITNAVICTGIAIRR22 pKa = 11.84VVTFRR27 pKa = 11.84RR28 pKa = 11.84NGSQHH33 pKa = 6.12RR34 pKa = 11.84RR35 pKa = 11.84WGGWLAYY42 pKa = 9.48FLIVAAASIPVRR54 pKa = 11.84VVYY57 pKa = 9.65AIWLRR62 pKa = 11.84TPMAVDD68 pKa = 3.96LSEE71 pKa = 4.6VIINAVMLAAVIKK84 pKa = 9.58TRR86 pKa = 11.84GNVVQIFKK94 pKa = 10.41ISRR97 pKa = 11.84SKK99 pKa = 11.04HH100 pKa = 4.33GDD102 pKa = 2.88

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11701

40

837

220.8

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.196 ± 0.582

0.906 ± 0.138

5.923 ± 0.219

6.709 ± 0.474

3.222 ± 0.181

7.461 ± 0.296

1.513 ± 0.125

5.658 ± 0.325

5.085 ± 0.289

7.709 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.291

4.547 ± 0.291

3.837 ± 0.252

4.897 ± 0.376

6.461 ± 0.349

6.598 ± 0.426

5.444 ± 0.311

6.35 ± 0.268

1.718 ± 0.172

2.957 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski