Winkia neuii BV029A5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Winkia; Winkia neuii

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0YV40|K0YV40_9ACTO 2-oxoglutarate dehydrogenase E1 component OS=Winkia neuii BV029A5 OX=888439 GN=HMPREF9240_00757 PE=4 SV=1
MM1 pKa = 7.57TYY3 pKa = 10.6VIAQPCVDD11 pKa = 3.66VKK13 pKa = 11.25DD14 pKa = 3.94RR15 pKa = 11.84ACVDD19 pKa = 3.71EE20 pKa = 5.5CPVDD24 pKa = 4.39CIYY27 pKa = 11.16EE28 pKa = 4.33GARR31 pKa = 11.84TLYY34 pKa = 9.75IHH36 pKa = 7.47PDD38 pKa = 3.07EE39 pKa = 5.5CVDD42 pKa = 4.38CGACEE47 pKa = 3.97PVCPTEE53 pKa = 4.64AIFYY57 pKa = 10.46EE58 pKa = 4.79DD59 pKa = 5.0DD60 pKa = 5.13LPEE63 pKa = 4.19EE64 pKa = 3.78WSDD67 pKa = 3.67YY68 pKa = 10.44YY69 pKa = 10.92RR70 pKa = 11.84ANTDD74 pKa = 3.76FFNLKK79 pKa = 10.43DD80 pKa = 3.67LGSPGGAAAVGPVDD94 pKa = 4.37YY95 pKa = 9.78DD96 pKa = 3.65DD97 pKa = 5.25PMIAEE102 pKa = 5.46LPPQAA107 pKa = 4.94

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0Z1F4|K0Z1F4_9ACTO UPF0234 protein HMPREF9240_01402 OS=Winkia neuii BV029A5 OX=888439 GN=HMPREF9240_01402 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1934

0

1934

650714

29

2228

336.5

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.927 ± 0.079

0.841 ± 0.017

5.465 ± 0.053

5.92 ± 0.068

3.218 ± 0.039

8.367 ± 0.047

1.904 ± 0.02

5.053 ± 0.04

4.528 ± 0.05

9.707 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.024

2.765 ± 0.03

4.931 ± 0.036

3.759 ± 0.036

5.822 ± 0.051

6.047 ± 0.036

5.655 ± 0.036

8.065 ± 0.049

1.394 ± 0.022

2.361 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski