Devosia sp. DBB001
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4502 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A024K8I7|A0A024K8I7_9RHIZ CblY a non-orthologous displasment for Alpha-rib azole-5'-phosphate phosphatase OS=Devosia sp. DBB001 OX=1380412 PE=4 SV=1
MM1 pKa = 8.02 AIGRR5 pKa = 11.84 LLLNSRR11 pKa = 11.84 LGFALIGALLCSPFISIEE29 pKa = 3.63 PAYY32 pKa = 10.74 ADD34 pKa = 4.18 LRR36 pKa = 11.84 VCNEE40 pKa = 3.77 TGNQVSIALGYY51 pKa = 9.19 RR52 pKa = 11.84 AEE54 pKa = 4.78 RR55 pKa = 11.84 GWQSEE60 pKa = 4.61 GWWVATPQQCAVVYY74 pKa = 10.65 QGDD77 pKa = 4.02 LNSRR81 pKa = 11.84 FYY83 pKa = 11.29 YY84 pKa = 10.45 LYY86 pKa = 10.6 VADD89 pKa = 5.84 DD90 pKa = 3.61 IGGGAWDD97 pKa = 4.22 GSVYY101 pKa = 9.99 MCTRR105 pKa = 11.84 DD106 pKa = 3.19 EE107 pKa = 4.38 SFTIFGVEE115 pKa = 3.75 DD116 pKa = 3.75 CLARR120 pKa = 11.84 GYY122 pKa = 10.83 EE123 pKa = 3.73 RR124 pKa = 11.84 TGFFEE129 pKa = 4.99 VDD131 pKa = 3.17 TQNRR135 pKa = 11.84 TDD137 pKa = 2.99 WTLQLTEE144 pKa = 4.43 NRR146 pKa = 11.84 DD147 pKa = 4.22 AIPEE151 pKa = 4.23 GDD153 pKa = 4.12 DD154 pKa = 3.41 ATGEE158 pKa = 4.11 PLGDD162 pKa = 3.63 EE163 pKa = 4.51 TGGVPPDD170 pKa = 3.84 QQDD173 pKa = 3.55 PPPPDD178 pKa = 3.44 EE179 pKa = 4.27 TTPAPEE185 pKa = 4.6 GPAAIQGSDD194 pKa = 2.77
Molecular weight: 21.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.63
ProMoST 3.948
Dawson 3.821
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.948
Patrickios 1.074
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A024KHT6|A0A024KHT6_9RHIZ Putative diheme cytochrome c-553 OS=Devosia sp. DBB001 OX=1380412 PE=4 SV=1
MM1 pKa = 7.43 RR2 pKa = 11.84 CARR5 pKa = 11.84 RR6 pKa = 11.84 ATDD9 pKa = 3.25 RR10 pKa = 11.84 TSRR13 pKa = 11.84 ATRR16 pKa = 11.84 RR17 pKa = 11.84 GVRR20 pKa = 11.84 TTRR23 pKa = 11.84 RR24 pKa = 11.84 TGRR27 pKa = 11.84 IIHH30 pKa = 6.34 RR31 pKa = 11.84 AARR34 pKa = 11.84 AAPWAHH40 pKa = 4.59 TTSRR44 pKa = 11.84 AAHH47 pKa = 5.3 TARR50 pKa = 11.84 RR51 pKa = 11.84 ARR53 pKa = 11.84 TTSRR57 pKa = 11.84 TTRR60 pKa = 11.84 ATHH63 pKa = 6.68 RR64 pKa = 11.84 ARR66 pKa = 11.84 HH67 pKa = 4.56 ICRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 NAACLCGRR80 pKa = 11.84 AGIGSRR86 pKa = 11.84 LL87 pKa = 3.42
Molecular weight: 9.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 10.745
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 12.998
Rodwell 12.018
Grimsley 12.559
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.74
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4502
0
4502
1360218
37
2687
302.1
32.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.292 ± 0.046
0.657 ± 0.011
5.679 ± 0.03
5.715 ± 0.038
3.855 ± 0.027
8.567 ± 0.034
1.918 ± 0.016
5.453 ± 0.026
3.326 ± 0.031
10.158 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.016
2.865 ± 0.025
5.14 ± 0.029
3.167 ± 0.021
6.547 ± 0.041
5.399 ± 0.026
5.459 ± 0.033
7.668 ± 0.029
1.344 ± 0.017
2.335 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here