Rickettsiella grylli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Rickettsiella

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8PQB9|A8PQB9_9COXI Beta sliding clamp OS=Rickettsiella grylli OX=59196 GN=dnaN PE=3 SV=1
MM1 pKa = 7.46TGGAIQVGRR10 pKa = 11.84GDD12 pKa = 4.68EE13 pKa = 4.02ISASNDD19 pKa = 2.92GGSVMFQGGTMCTIDD34 pKa = 4.84GIGVSCSS41 pKa = 3.03

Molecular weight: 3.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8PQG7|A8PQG7_9COXI 50S ribosomal subunit assembly factor BipA OS=Rickettsiella grylli OX=59196 GN=typA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.32QPSNLKK11 pKa = 10.19RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.2KK26 pKa = 10.43GRR28 pKa = 11.84LIIKK32 pKa = 9.89RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.53GRR39 pKa = 11.84FRR41 pKa = 11.84LTAA44 pKa = 4.0

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1372

0

1372

442456

37

4210

322.5

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.199 ± 0.065

1.215 ± 0.025

4.369 ± 0.047

5.45 ± 0.064

4.83 ± 0.058

5.847 ± 0.089

2.845 ± 0.037

7.734 ± 0.058

6.636 ± 0.074

11.704 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.028

4.895 ± 0.057

4.111 ± 0.034

4.589 ± 0.059

4.735 ± 0.053

6.268 ± 0.056

5.42 ± 0.05

5.508 ± 0.058

1.143 ± 0.025

3.399 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski