Pothos latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q84832|Q84832_9TOMB Capsid protein OS=Pothos latent virus OX=44562 PE=3 SV=1
MM1 pKa = 7.54ANWCVKK7 pKa = 10.31VLMFPFVTVYY17 pKa = 10.33TIVTSLGRR25 pKa = 11.84GIRR28 pKa = 11.84RR29 pKa = 11.84GFRR32 pKa = 11.84ACVDD36 pKa = 3.67EE37 pKa = 4.36VEE39 pKa = 4.77NIYY42 pKa = 11.39GDD44 pKa = 3.37MCAGFRR50 pKa = 11.84VALSKK55 pKa = 11.14LSVSWIVVVGMIFVLNSLVVGLVPTLTMAAIIAACVGAKK94 pKa = 10.1YY95 pKa = 10.28GGRR98 pKa = 11.84VPRR101 pKa = 11.84YY102 pKa = 8.99IRR104 pKa = 11.84AHH106 pKa = 5.44VDD108 pKa = 4.04RR109 pKa = 11.84IRR111 pKa = 11.84KK112 pKa = 8.57SWEE115 pKa = 3.68KK116 pKa = 10.95GVDD119 pKa = 4.39DD120 pKa = 5.8DD121 pKa = 5.63DD122 pKa = 4.72CCAPLPEE129 pKa = 4.5TSLEE133 pKa = 4.02RR134 pKa = 11.84VPVKK138 pKa = 10.31LRR140 pKa = 11.84PRR142 pKa = 11.84LACKK146 pKa = 9.74IAVRR150 pKa = 11.84AIAKK154 pKa = 9.99VGLLKK159 pKa = 10.63RR160 pKa = 11.84SEE162 pKa = 4.34ANSMVYY168 pKa = 9.97QRR170 pKa = 11.84VCLDD174 pKa = 3.14VMEE177 pKa = 4.42SMKK180 pKa = 10.07MRR182 pKa = 11.84WHH184 pKa = 6.99DD185 pKa = 3.63RR186 pKa = 11.84LVILPQAVLACLEE199 pKa = 4.01RR200 pKa = 11.84PEE202 pKa = 4.2EE203 pKa = 4.11VEE205 pKa = 4.1EE206 pKa = 4.07VMKK209 pKa = 10.92AIEE212 pKa = 4.48ASCKK216 pKa = 10.7GPFDD220 pKa = 4.02VYY222 pKa = 10.13XGCLLQHH229 pKa = 6.83RR230 pKa = 11.84GYY232 pKa = 8.69NTAITSVIPDD242 pKa = 3.54GVLLNHH248 pKa = 6.85EE249 pKa = 4.32GMVVRR254 pKa = 11.84KK255 pKa = 8.62GAPITNEE262 pKa = 3.51RR263 pKa = 11.84RR264 pKa = 11.84WYY266 pKa = 10.71SFAGYY271 pKa = 10.48ASTYY275 pKa = 10.04EE276 pKa = 4.24YY277 pKa = 10.18IVHH280 pKa = 6.48NSSLVNVCRR289 pKa = 11.84GLVEE293 pKa = 4.85RR294 pKa = 11.84VFCVVRR300 pKa = 11.84DD301 pKa = 4.08GKK303 pKa = 9.68LQRR306 pKa = 11.84PLRR309 pKa = 11.84PKK311 pKa = 10.72NNVFEE316 pKa = 4.71RR317 pKa = 11.84KK318 pKa = 9.36LGNIGRR324 pKa = 11.84KK325 pKa = 8.33ISSIVGYY332 pKa = 9.29CPAMTRR338 pKa = 11.84SEE340 pKa = 4.24FCDD343 pKa = 3.45SYY345 pKa = 11.35SGTRR349 pKa = 11.84RR350 pKa = 11.84ATYY353 pKa = 9.75EE354 pKa = 3.85RR355 pKa = 11.84ARR357 pKa = 11.84LSLDD361 pKa = 3.18VLPCTRR367 pKa = 11.84KK368 pKa = 9.84DD369 pKa = 3.24AYY371 pKa = 11.22LKK373 pKa = 10.07TFVKK377 pKa = 10.54AEE379 pKa = 4.43KK380 pKa = 10.4INITLKK386 pKa = 10.25PDD388 pKa = 3.82PAPRR392 pKa = 11.84VIQPRR397 pKa = 11.84DD398 pKa = 3.11PRR400 pKa = 11.84INVEE404 pKa = 3.57VGRR407 pKa = 11.84YY408 pKa = 7.94LKK410 pKa = 10.36PLEE413 pKa = 4.31PRR415 pKa = 11.84LMKK418 pKa = 10.74AIDD421 pKa = 3.85KK422 pKa = 10.71LWGEE426 pKa = 3.96KK427 pKa = 8.64TAIKK431 pKa = 10.27GYY433 pKa = 6.52TVEE436 pKa = 4.31KK437 pKa = 10.33VGEE440 pKa = 4.02ILHH443 pKa = 5.5QKK445 pKa = 8.49SLRR448 pKa = 11.84FKK450 pKa = 9.78NPCFVGLDD458 pKa = 3.29ASRR461 pKa = 11.84FDD463 pKa = 3.64QHH465 pKa = 7.34CSRR468 pKa = 11.84QALEE472 pKa = 4.51WEE474 pKa = 4.17HH475 pKa = 7.25SVYY478 pKa = 10.83NAIFRR483 pKa = 11.84DD484 pKa = 4.23PYY486 pKa = 10.98LSEE489 pKa = 4.3LLTWQIDD496 pKa = 4.22NVGTAYY502 pKa = 10.93LKK504 pKa = 10.95DD505 pKa = 3.2GFVRR509 pKa = 11.84YY510 pKa = 9.28RR511 pKa = 11.84VDD513 pKa = 3.25GCRR516 pKa = 11.84MSGDD520 pKa = 3.85MNTSMGNYY528 pKa = 10.0LIMSCLVYY536 pKa = 9.28QFCKK540 pKa = 10.64DD541 pKa = 3.18IGVDD545 pKa = 4.14ASLANCGDD553 pKa = 3.62DD554 pKa = 3.56CVLYY558 pKa = 10.76LEE560 pKa = 5.81KK561 pKa = 10.79EE562 pKa = 4.2DD563 pKa = 4.5LPKK566 pKa = 10.98LKK568 pKa = 10.47ALPDD572 pKa = 3.31WFEE575 pKa = 4.2KK576 pKa = 9.77MGYY579 pKa = 7.98SMKK582 pKa = 10.21VEE584 pKa = 3.95KK585 pKa = 10.24PVFTVEE591 pKa = 4.44EE592 pKa = 4.2IEE594 pKa = 4.63FCQQHH599 pKa = 6.18PVQLSRR605 pKa = 11.84GYY607 pKa = 11.23VMVRR611 pKa = 11.84RR612 pKa = 11.84PDD614 pKa = 3.14VCLTKK619 pKa = 10.82DD620 pKa = 3.51LLPFVRR626 pKa = 11.84GGMTTEE632 pKa = 4.28RR633 pKa = 11.84LQRR636 pKa = 11.84WLYY639 pKa = 8.84AQHH642 pKa = 7.39DD643 pKa = 4.31GGLALAGDD651 pKa = 4.63CPVLGAFYY659 pKa = 10.66QRR661 pKa = 11.84FPSGEE666 pKa = 3.99SHH668 pKa = 6.95GEE670 pKa = 3.5LSEE673 pKa = 4.15YY674 pKa = 11.13SDD676 pKa = 3.14AHH678 pKa = 6.14KK679 pKa = 10.65FKK681 pKa = 10.88AGKK684 pKa = 9.78QYY686 pKa = 11.72GEE688 pKa = 3.97ITSLARR694 pKa = 11.84YY695 pKa = 8.53SFWLAFGITPDD706 pKa = 3.32EE707 pKa = 3.92QLAIEE712 pKa = 4.68RR713 pKa = 11.84DD714 pKa = 3.68LMNWTPNVVLGSCDD728 pKa = 3.64PRR730 pKa = 11.84PTLLDD735 pKa = 3.46YY736 pKa = 11.46CSDD739 pKa = 3.48NN740 pKa = 3.65

Molecular weight: 83.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q84834|Q84834_9TOMB Isoform of Q84830 Orf protein OS=Pothos latent virus OX=44562 GN=orf PE=4 SV=1
MM1 pKa = 7.97EE2 pKa = 4.88IQSLDD7 pKa = 3.91GILGEE12 pKa = 4.42EE13 pKa = 4.03LAIQNEE19 pKa = 4.45VKK21 pKa = 10.6KK22 pKa = 10.69ILLSHH27 pKa = 6.56KK28 pKa = 5.62TTKK31 pKa = 10.62AILPLAPISQFSKK44 pKa = 10.16WKK46 pKa = 9.54IPKK49 pKa = 9.4QGFYY53 pKa = 11.32APIDD57 pKa = 3.67VKK59 pKa = 10.92FVLTPHH65 pKa = 6.09ISEE68 pKa = 4.33RR69 pKa = 11.84AQVRR73 pKa = 11.84GVVKK77 pKa = 10.64LVDD80 pKa = 3.88SRR82 pKa = 11.84DD83 pKa = 3.61LSPSRR88 pKa = 11.84EE89 pKa = 4.0LYY91 pKa = 10.0RR92 pKa = 11.84SKK94 pKa = 10.77EE95 pKa = 3.86FNIGHH100 pKa = 6.45GLVIEE105 pKa = 5.22GSQLPFCLPVGDD117 pKa = 4.68YY118 pKa = 10.33PLQFEE123 pKa = 4.7VTVLQSQFRR132 pKa = 11.84EE133 pKa = 4.09TANLYY138 pKa = 7.62STSVEE143 pKa = 3.48WRR145 pKa = 11.84MMSSTTPLSRR155 pKa = 11.84VRR157 pKa = 11.84SVMGAAQQPAMKK169 pKa = 9.75LQPNFKK175 pKa = 9.8MSLEE179 pKa = 4.08SSKK182 pKa = 11.21GGGMKK187 pKa = 10.02SHH189 pKa = 6.14QKK191 pKa = 10.6KK192 pKa = 9.25NSKK195 pKa = 10.18PNGHH199 pKa = 5.84SRR201 pKa = 11.84RR202 pKa = 11.84GNLSGEE208 pKa = 4.43VGGSSSSLPSGAQTGEE224 pKa = 4.28WIDD227 pKa = 3.52NDD229 pKa = 3.87YY230 pKa = 11.56GDD232 pKa = 4.61SLSEE236 pKa = 3.98YY237 pKa = 10.28SGVSAA242 pKa = 5.52

Molecular weight: 26.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1707

130

740

341.4

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.971 ± 0.826

2.929 ± 0.663

4.452 ± 0.548

5.214 ± 0.421

3.515 ± 0.195

7.616 ± 0.534

1.699 ± 0.28

5.331 ± 0.311

5.097 ± 0.505

8.729 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.432

3.574 ± 0.61

4.979 ± 0.164

3.281 ± 0.774

6.678 ± 0.781

7.44 ± 1.47

5.39 ± 1.096

9.315 ± 0.979

1.465 ± 0.119

3.574 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski