Bacillus phage vB_BthS-HD29phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P1MB67|A0A5P1MB67_9CAUD Uncharacterized protein OS=Bacillus phage vB_BthS-HD29phi OX=2593661 PE=4 SV=1
MM1 pKa = 8.39IEE3 pKa = 4.07EE4 pKa = 4.46SQFSLMIVALVICIVSLTVYY24 pKa = 10.57FLDD27 pKa = 4.12KK28 pKa = 10.56PIEE31 pKa = 4.08KK32 pKa = 10.03FIKK35 pKa = 10.63DD36 pKa = 3.82DD37 pKa = 4.5DD38 pKa = 4.81SDD40 pKa = 3.78VV41 pKa = 3.33

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P1MAN0|A0A5P1MAN0_9CAUD Sigma-70 family RNA polymerase sigma factor OS=Bacillus phage vB_BthS-HD29phi OX=2593661 PE=4 SV=1
MM1 pKa = 7.72GNLKK5 pKa = 10.1KK6 pKa = 10.64KK7 pKa = 10.04KK8 pKa = 9.02IKK10 pKa = 10.19KK11 pKa = 9.99AIDD14 pKa = 2.96RR15 pKa = 11.84RR16 pKa = 11.84ARR18 pKa = 11.84AMDD21 pKa = 3.47KK22 pKa = 10.92EE23 pKa = 4.3RR24 pKa = 11.84VTNAWRR30 pKa = 11.84NIFVQAGIVKK40 pKa = 10.27

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

9468

40

1173

193.2

22.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.925 ± 0.485

0.877 ± 0.197

5.714 ± 0.258

8.281 ± 0.469

4.225 ± 0.217

6.242 ± 0.401

1.637 ± 0.221

7.33 ± 0.239

9.485 ± 0.417

8.428 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.021 ± 0.196

5.798 ± 0.234

2.693 ± 0.189

4.066 ± 0.309

4.204 ± 0.314

5.492 ± 0.213

5.165 ± 0.301

6.517 ± 0.274

1.278 ± 0.202

3.623 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski