Amino acid dipepetide frequency for Bacillus phage vB_BthS-HD29phi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.859AlaAla: 4.859 ± 1.368
0.317AlaCys: 0.317 ± 0.172
3.486AlaAsp: 3.486 ± 0.608
4.965AlaGlu: 4.965 ± 0.778
2.113AlaPhe: 2.113 ± 0.425
3.697AlaGly: 3.697 ± 0.675
1.056AlaHis: 1.056 ± 0.383
4.12AlaIle: 4.12 ± 0.5
5.282AlaLys: 5.282 ± 0.842
5.07AlaLeu: 5.07 ± 0.786
1.901AlaMet: 1.901 ± 0.423
2.007AlaAsn: 2.007 ± 0.553
1.162AlaPro: 1.162 ± 0.242
3.38AlaGln: 3.38 ± 0.571
2.746AlaArg: 2.746 ± 0.521
3.063AlaSer: 3.063 ± 0.515
2.852AlaThr: 2.852 ± 0.605
3.697AlaVal: 3.697 ± 0.651
1.69AlaTrp: 1.69 ± 0.589
1.796AlaTyr: 1.796 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.423CysAla: 0.423 ± 0.242
0.211CysCys: 0.211 ± 0.142
0.739CysAsp: 0.739 ± 0.293
0.739CysGlu: 0.739 ± 0.328
0.106CysPhe: 0.106 ± 0.111
0.634CysGly: 0.634 ± 0.278
0.317CysHis: 0.317 ± 0.232
0.739CysIle: 0.739 ± 0.249
0.634CysLys: 0.634 ± 0.313
0.211CysLeu: 0.211 ± 0.164
0.106CysMet: 0.106 ± 0.101
0.211CysAsn: 0.211 ± 0.148
0.634CysPro: 0.634 ± 0.334
0.634CysGln: 0.634 ± 0.288
0.211CysArg: 0.211 ± 0.171
0.528CysSer: 0.528 ± 0.211
0.211CysThr: 0.211 ± 0.171
0.634CysVal: 0.634 ± 0.285
0.0CysTrp: 0.0 ± 0.0
0.845CysTyr: 0.845 ± 0.348
0.0CysXaa: 0.0 ± 0.0
Asp
3.697AspAla: 3.697 ± 0.729
0.317AspCys: 0.317 ± 0.171
3.486AspAsp: 3.486 ± 0.59
4.859AspGlu: 4.859 ± 0.818
2.641AspPhe: 2.641 ± 0.512
3.908AspGly: 3.908 ± 0.527
0.634AspHis: 0.634 ± 0.331
4.859AspIle: 4.859 ± 0.792
4.12AspLys: 4.12 ± 0.531
4.331AspLeu: 4.331 ± 0.632
2.324AspMet: 2.324 ± 0.481
3.063AspAsn: 3.063 ± 0.581
2.007AspPro: 2.007 ± 0.499
1.69AspGln: 1.69 ± 0.404
2.535AspArg: 2.535 ± 0.516
2.852AspSer: 2.852 ± 0.408
2.641AspThr: 2.641 ± 0.497
4.225AspVal: 4.225 ± 0.637
0.951AspTrp: 0.951 ± 0.413
2.007AspTyr: 2.007 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
5.387GluAla: 5.387 ± 0.831
0.634GluCys: 0.634 ± 0.285
4.753GluAsp: 4.753 ± 0.883
7.5GluGlu: 7.5 ± 1.195
4.014GluPhe: 4.014 ± 0.582
3.908GluGly: 3.908 ± 0.523
2.113GluHis: 2.113 ± 0.547
6.232GluIle: 6.232 ± 0.906
8.345GluLys: 8.345 ± 1.101
7.5GluLeu: 7.5 ± 0.953
3.063GluMet: 3.063 ± 0.441
5.176GluAsn: 5.176 ± 0.723
1.69GluPro: 1.69 ± 0.436
4.331GluGln: 4.331 ± 0.704
2.535GluArg: 2.535 ± 0.525
3.486GluSer: 3.486 ± 0.674
3.697GluThr: 3.697 ± 0.569
4.753GluVal: 4.753 ± 0.757
0.634GluTrp: 0.634 ± 0.27
3.063GluTyr: 3.063 ± 0.731
0.0GluXaa: 0.0 ± 0.0
Phe
2.535PheAla: 2.535 ± 0.632
0.423PheCys: 0.423 ± 0.206
2.218PheAsp: 2.218 ± 0.429
3.591PheGlu: 3.591 ± 0.633
1.796PhePhe: 1.796 ± 0.641
3.275PheGly: 3.275 ± 0.579
0.528PheHis: 0.528 ± 0.255
3.169PheIle: 3.169 ± 0.474
4.436PheLys: 4.436 ± 0.665
2.852PheLeu: 2.852 ± 0.508
0.845PheMet: 0.845 ± 0.276
2.429PheAsn: 2.429 ± 0.497
1.373PhePro: 1.373 ± 0.372
1.373PheGln: 1.373 ± 0.453
1.268PheArg: 1.268 ± 0.329
3.063PheSer: 3.063 ± 0.558
2.007PheThr: 2.007 ± 0.469
2.852PheVal: 2.852 ± 0.766
0.528PheTrp: 0.528 ± 0.217
1.69PheTyr: 1.69 ± 0.489
0.0PheXaa: 0.0 ± 0.0
Gly
4.12GlyAla: 4.12 ± 0.81
1.162GlyCys: 1.162 ± 0.426
3.063GlyAsp: 3.063 ± 0.584
5.176GlyGlu: 5.176 ± 0.653
3.169GlyPhe: 3.169 ± 0.548
4.12GlyGly: 4.12 ± 0.815
0.845GlyHis: 0.845 ± 0.32
4.859GlyIle: 4.859 ± 0.858
5.387GlyLys: 5.387 ± 0.88
5.387GlyLeu: 5.387 ± 0.732
1.796GlyMet: 1.796 ± 0.351
3.38GlyAsn: 3.38 ± 0.764
0.634GlyPro: 0.634 ± 0.214
2.218GlyGln: 2.218 ± 0.64
2.852GlyArg: 2.852 ± 0.591
2.429GlySer: 2.429 ± 0.506
3.275GlyThr: 3.275 ± 0.608
5.176GlyVal: 5.176 ± 0.715
1.056GlyTrp: 1.056 ± 0.271
2.324GlyTyr: 2.324 ± 0.676
0.0GlyXaa: 0.0 ± 0.0
His
0.423HisAla: 0.423 ± 0.197
0.211HisCys: 0.211 ± 0.155
0.634HisAsp: 0.634 ± 0.28
0.951HisGlu: 0.951 ± 0.357
0.951HisPhe: 0.951 ± 0.315
0.845HisGly: 0.845 ± 0.382
0.528HisHis: 0.528 ± 0.293
1.162HisIle: 1.162 ± 0.335
2.113HisLys: 2.113 ± 0.522
1.69HisLeu: 1.69 ± 0.562
0.106HisMet: 0.106 ± 0.109
0.739HisAsn: 0.739 ± 0.237
0.423HisPro: 0.423 ± 0.212
0.739HisGln: 0.739 ± 0.335
0.845HisArg: 0.845 ± 0.342
0.739HisSer: 0.739 ± 0.29
0.634HisThr: 0.634 ± 0.267
1.796HisVal: 1.796 ± 0.392
0.317HisTrp: 0.317 ± 0.174
0.528HisTyr: 0.528 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
4.648IleAla: 4.648 ± 0.671
0.951IleCys: 0.951 ± 0.306
4.331IleAsp: 4.331 ± 0.658
7.183IleGlu: 7.183 ± 1.337
2.324IlePhe: 2.324 ± 0.578
4.12IleGly: 4.12 ± 0.823
0.951IleHis: 0.951 ± 0.339
3.803IleIle: 3.803 ± 0.648
6.443IleLys: 6.443 ± 0.896
5.598IleLeu: 5.598 ± 0.626
2.218IleMet: 2.218 ± 0.518
4.648IleAsn: 4.648 ± 0.757
2.641IlePro: 2.641 ± 0.497
2.852IleGln: 2.852 ± 0.705
3.591IleArg: 3.591 ± 0.652
3.697IleSer: 3.697 ± 0.708
5.07IleThr: 5.07 ± 0.849
4.965IleVal: 4.965 ± 0.672
0.528IleTrp: 0.528 ± 0.247
2.746IleTyr: 2.746 ± 0.469
0.0IleXaa: 0.0 ± 0.0
Lys
5.704LysAla: 5.704 ± 0.756
0.634LysCys: 0.634 ± 0.337
4.859LysAsp: 4.859 ± 0.571
8.873LysGlu: 8.873 ± 1.354
2.958LysPhe: 2.958 ± 0.535
6.443LysGly: 6.443 ± 0.813
1.901LysHis: 1.901 ± 0.512
6.127LysIle: 6.127 ± 0.887
12.253LysLys: 12.253 ± 1.521
7.077LysLeu: 7.077 ± 1.16
3.063LysMet: 3.063 ± 0.553
5.176LysAsn: 5.176 ± 0.775
1.901LysPro: 1.901 ± 0.584
4.225LysGln: 4.225 ± 0.675
4.225LysArg: 4.225 ± 0.702
4.12LysSer: 4.12 ± 0.553
6.127LysThr: 6.127 ± 0.843
5.704LysVal: 5.704 ± 0.891
0.739LysTrp: 0.739 ± 0.278
2.641LysTyr: 2.641 ± 0.522
0.0LysXaa: 0.0 ± 0.0
Leu
5.387LeuAla: 5.387 ± 0.981
0.845LeuCys: 0.845 ± 0.41
5.81LeuAsp: 5.81 ± 0.771
7.605LeuGlu: 7.605 ± 1.116
3.697LeuPhe: 3.697 ± 0.594
6.443LeuGly: 6.443 ± 0.793
0.845LeuHis: 0.845 ± 0.282
5.387LeuIle: 5.387 ± 0.796
6.866LeuLys: 6.866 ± 0.905
6.76LeuLeu: 6.76 ± 0.84
2.535LeuMet: 2.535 ± 0.562
5.282LeuAsn: 5.282 ± 0.772
2.113LeuPro: 2.113 ± 0.409
3.38LeuGln: 3.38 ± 0.748
3.803LeuArg: 3.803 ± 0.562
4.753LeuSer: 4.753 ± 0.722
5.176LeuThr: 5.176 ± 0.672
4.331LeuVal: 4.331 ± 0.553
0.951LeuTrp: 0.951 ± 0.423
2.324LeuTyr: 2.324 ± 0.548
0.0LeuXaa: 0.0 ± 0.0
Met
1.373MetAla: 1.373 ± 0.426
0.317MetCys: 0.317 ± 0.204
2.535MetAsp: 2.535 ± 0.454
2.429MetGlu: 2.429 ± 0.558
0.634MetPhe: 0.634 ± 0.25
1.584MetGly: 1.584 ± 0.487
0.423MetHis: 0.423 ± 0.207
2.324MetIle: 2.324 ± 0.518
2.746MetLys: 2.746 ± 0.53
2.852MetLeu: 2.852 ± 0.556
0.951MetMet: 0.951 ± 0.265
1.69MetAsn: 1.69 ± 0.409
1.162MetPro: 1.162 ± 0.311
0.528MetGln: 0.528 ± 0.264
1.373MetArg: 1.373 ± 0.334
2.535MetSer: 2.535 ± 0.402
1.479MetThr: 1.479 ± 0.357
1.69MetVal: 1.69 ± 0.401
0.0MetTrp: 0.0 ± 0.0
1.584MetTyr: 1.584 ± 0.64
0.0MetXaa: 0.0 ± 0.0
Asn
3.591AsnAla: 3.591 ± 0.774
0.423AsnCys: 0.423 ± 0.265
2.535AsnAsp: 2.535 ± 0.457
4.965AsnGlu: 4.965 ± 0.622
2.535AsnPhe: 2.535 ± 0.519
4.859AsnGly: 4.859 ± 0.977
0.634AsnHis: 0.634 ± 0.221
4.542AsnIle: 4.542 ± 0.648
5.598AsnLys: 5.598 ± 0.806
5.282AsnLeu: 5.282 ± 0.704
1.373AsnMet: 1.373 ± 0.348
3.063AsnAsn: 3.063 ± 0.683
1.796AsnPro: 1.796 ± 0.419
1.901AsnGln: 1.901 ± 0.461
2.324AsnArg: 2.324 ± 0.534
3.275AsnSer: 3.275 ± 0.607
2.535AsnThr: 2.535 ± 0.48
3.697AsnVal: 3.697 ± 0.651
0.845AsnTrp: 0.845 ± 0.38
2.218AsnTyr: 2.218 ± 0.479
0.0AsnXaa: 0.0 ± 0.0
Pro
1.056ProAla: 1.056 ± 0.345
0.0ProCys: 0.0 ± 0.0
1.584ProAsp: 1.584 ± 0.646
1.479ProGlu: 1.479 ± 0.365
1.479ProPhe: 1.479 ± 0.381
1.056ProGly: 1.056 ± 0.347
0.528ProHis: 0.528 ± 0.206
2.746ProIle: 2.746 ± 0.493
3.063ProLys: 3.063 ± 0.532
2.852ProLeu: 2.852 ± 0.513
0.634ProMet: 0.634 ± 0.292
1.268ProAsn: 1.268 ± 0.422
0.528ProPro: 0.528 ± 0.196
0.317ProGln: 0.317 ± 0.176
0.634ProArg: 0.634 ± 0.233
2.007ProSer: 2.007 ± 0.47
2.324ProThr: 2.324 ± 0.569
2.535ProVal: 2.535 ± 0.533
0.0ProTrp: 0.0 ± 0.0
0.845ProTyr: 0.845 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
3.063GlnAla: 3.063 ± 0.754
0.106GlnCys: 0.106 ± 0.092
1.584GlnAsp: 1.584 ± 0.292
3.275GlnGlu: 3.275 ± 0.724
2.852GlnPhe: 2.852 ± 0.615
2.007GlnGly: 2.007 ± 0.455
0.739GlnHis: 0.739 ± 0.303
3.38GlnIle: 3.38 ± 0.57
2.535GlnLys: 2.535 ± 0.534
3.803GlnLeu: 3.803 ± 0.536
1.268GlnMet: 1.268 ± 0.353
2.746GlnAsn: 2.746 ± 0.577
0.951GlnPro: 0.951 ± 0.292
1.69GlnGln: 1.69 ± 0.386
2.218GlnArg: 2.218 ± 0.499
2.535GlnSer: 2.535 ± 0.63
1.479GlnThr: 1.479 ± 0.501
2.218GlnVal: 2.218 ± 0.425
0.951GlnTrp: 0.951 ± 0.333
1.268GlnTyr: 1.268 ± 0.376
0.0GlnXaa: 0.0 ± 0.0
Arg
1.584ArgAla: 1.584 ± 0.355
0.317ArgCys: 0.317 ± 0.17
2.429ArgAsp: 2.429 ± 0.525
3.275ArgGlu: 3.275 ± 0.678
0.845ArgPhe: 0.845 ± 0.282
2.218ArgGly: 2.218 ± 0.536
1.056ArgHis: 1.056 ± 0.354
3.063ArgIle: 3.063 ± 0.683
3.486ArgLys: 3.486 ± 0.55
2.958ArgLeu: 2.958 ± 0.657
2.324ArgMet: 2.324 ± 0.459
2.641ArgAsn: 2.641 ± 0.523
1.373ArgPro: 1.373 ± 0.426
1.479ArgGln: 1.479 ± 0.382
2.324ArgArg: 2.324 ± 0.57
2.535ArgSer: 2.535 ± 0.572
1.796ArgThr: 1.796 ± 0.475
3.697ArgVal: 3.697 ± 0.557
0.845ArgTrp: 0.845 ± 0.279
1.796ArgTyr: 1.796 ± 0.691
0.0ArgXaa: 0.0 ± 0.0
Ser
3.169SerAla: 3.169 ± 0.621
0.106SerCys: 0.106 ± 0.086
3.591SerAsp: 3.591 ± 0.654
3.697SerGlu: 3.697 ± 0.875
2.113SerPhe: 2.113 ± 0.391
3.486SerGly: 3.486 ± 0.659
0.951SerHis: 0.951 ± 0.338
5.07SerIle: 5.07 ± 0.904
4.859SerLys: 4.859 ± 0.603
5.387SerLeu: 5.387 ± 0.632
1.162SerMet: 1.162 ± 0.393
3.591SerAsn: 3.591 ± 0.72
1.69SerPro: 1.69 ± 0.437
2.218SerGln: 2.218 ± 0.526
1.584SerArg: 1.584 ± 0.382
2.324SerSer: 2.324 ± 0.57
3.486SerThr: 3.486 ± 0.719
2.958SerVal: 2.958 ± 0.501
0.845SerTrp: 0.845 ± 0.292
2.641SerTyr: 2.641 ± 0.618
0.0SerXaa: 0.0 ± 0.0
Thr
3.275ThrAla: 3.275 ± 0.651
0.528ThrCys: 0.528 ± 0.244
3.275ThrAsp: 3.275 ± 0.704
3.591ThrGlu: 3.591 ± 0.571
2.429ThrPhe: 2.429 ± 0.569
3.803ThrGly: 3.803 ± 0.996
0.528ThrHis: 0.528 ± 0.242
4.12ThrIle: 4.12 ± 0.792
4.648ThrLys: 4.648 ± 0.79
4.648ThrLeu: 4.648 ± 0.569
1.479ThrMet: 1.479 ± 0.377
2.958ThrAsn: 2.958 ± 0.612
1.69ThrPro: 1.69 ± 0.424
2.641ThrGln: 2.641 ± 0.612
1.268ThrArg: 1.268 ± 0.323
2.746ThrSer: 2.746 ± 0.524
2.324ThrThr: 2.324 ± 0.609
3.275ThrVal: 3.275 ± 0.649
0.845ThrTrp: 0.845 ± 0.295
1.584ThrTyr: 1.584 ± 0.499
0.0ThrXaa: 0.0 ± 0.0
Val
2.852ValAla: 2.852 ± 0.58
0.423ValCys: 0.423 ± 0.215
3.697ValAsp: 3.697 ± 0.54
4.753ValGlu: 4.753 ± 0.606
2.852ValPhe: 2.852 ± 0.784
3.697ValGly: 3.697 ± 0.796
1.056ValHis: 1.056 ± 0.379
4.542ValIle: 4.542 ± 0.638
6.76ValLys: 6.76 ± 0.759
4.859ValLeu: 4.859 ± 0.633
2.113ValMet: 2.113 ± 0.578
4.014ValAsn: 4.014 ± 0.643
1.901ValPro: 1.901 ± 0.407
3.486ValGln: 3.486 ± 0.621
3.38ValArg: 3.38 ± 0.559
4.542ValSer: 4.542 ± 0.689
2.746ValThr: 2.746 ± 0.528
4.12ValVal: 4.12 ± 0.673
0.528ValTrp: 0.528 ± 0.17
2.852ValTyr: 2.852 ± 0.409
0.0ValXaa: 0.0 ± 0.0
Trp
0.528TrpAla: 0.528 ± 0.232
0.211TrpCys: 0.211 ± 0.151
0.739TrpAsp: 0.739 ± 0.253
1.584TrpGlu: 1.584 ± 0.463
0.317TrpPhe: 0.317 ± 0.153
0.528TrpGly: 0.528 ± 0.262
0.0TrpHis: 0.0 ± 0.0
0.951TrpIle: 0.951 ± 0.286
1.268TrpLys: 1.268 ± 0.286
1.373TrpLeu: 1.373 ± 0.472
0.106TrpMet: 0.106 ± 0.122
1.162TrpAsn: 1.162 ± 0.421
0.0TrpPro: 0.0 ± 0.0
0.211TrpGln: 0.211 ± 0.117
0.739TrpArg: 0.739 ± 0.268
1.268TrpSer: 1.268 ± 0.471
0.423TrpThr: 0.423 ± 0.275
0.528TrpVal: 0.528 ± 0.219
0.0TrpTrp: 0.0 ± 0.0
0.845TrpTyr: 0.845 ± 0.339
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.584TyrAla: 1.584 ± 0.469
0.634TyrCys: 0.634 ± 0.323
1.796TyrAsp: 1.796 ± 0.451
2.324TyrGlu: 2.324 ± 0.476
2.429TyrPhe: 2.429 ± 0.409
1.796TyrGly: 1.796 ± 0.448
0.739TyrHis: 0.739 ± 0.262
2.113TyrIle: 2.113 ± 0.331
4.014TyrLys: 4.014 ± 0.656
3.803TyrLeu: 3.803 ± 0.837
0.739TyrMet: 0.739 ± 0.299
2.746TyrAsn: 2.746 ± 0.623
1.268TyrPro: 1.268 ± 0.436
1.373TyrGln: 1.373 ± 0.254
1.584TyrArg: 1.584 ± 0.389
2.429TyrSer: 2.429 ± 0.628
1.373TyrThr: 1.373 ± 0.294
2.324TyrVal: 2.324 ± 0.5
0.528TyrTrp: 0.528 ± 0.218
0.634TyrTyr: 0.634 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (9468 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski