Catabacter hongkongensis
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M2NB90|A0A0M2NB90_9FIRM D-ribose pyranase OS=Catabacter hongkongensis OX=270498 GN=CHK_3076 PE=4 SV=1
MM1 pKa = 7.84 AFTEE5 pKa = 4.14 NEE7 pKa = 3.84 KK8 pKa = 10.13 TGEE11 pKa = 4.02 KK12 pKa = 10.61 NYY14 pKa = 10.2 FPDD17 pKa = 4.29 LKK19 pKa = 11.05 NIDD22 pKa = 4.59 EE23 pKa = 4.69 ILIQNPDD30 pKa = 3.63 LGCVVSNKK38 pKa = 9.7 ISVEE42 pKa = 4.14 GYY44 pKa = 10.38 EE45 pKa = 4.13 IGCMYY50 pKa = 10.38 RR51 pKa = 11.84 DD52 pKa = 3.64 EE53 pKa = 5.1 PLPDD57 pKa = 3.4 VADD60 pKa = 4.02 SGWVFLAGDD69 pKa = 3.72 EE70 pKa = 4.68 DD71 pKa = 4.88 ADD73 pKa = 4.36 YY74 pKa = 10.95 MNTPGNMSAFPLNTMAHH91 pKa = 6.45 YY92 pKa = 10.39 DD93 pKa = 3.59 PAVIPLLDD101 pKa = 4.23 ADD103 pKa = 4.76 VFTAYY108 pKa = 10.48 CRR110 pKa = 11.84 DD111 pKa = 3.55 EE112 pKa = 4.84 DD113 pKa = 4.14 GKK115 pKa = 10.0 LTLTEE120 pKa = 4.4 HH121 pKa = 6.44 FFDD124 pKa = 5.07 GADD127 pKa = 3.62 EE128 pKa = 4.42 EE129 pKa = 4.79 SLPDD133 pKa = 3.38
Molecular weight: 14.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.846
Patrickios 0.985
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A0M2NII0|A0A0M2NII0_9FIRM 50S ribosomal protein L1 OS=Catabacter hongkongensis OX=270498 GN=rplA PE=3 SV=1
MM1 pKa = 7.83 IITVLSRR8 pKa = 11.84 QRR10 pKa = 11.84 FRR12 pKa = 11.84 FRR14 pKa = 11.84 RR15 pKa = 11.84 EE16 pKa = 3.62 KK17 pKa = 8.02 ITAWRR22 pKa = 11.84 RR23 pKa = 11.84 IAARR27 pKa = 11.84 IARR30 pKa = 11.84 MKK32 pKa = 9.91 MLAGQSRR39 pKa = 11.84 PARR42 pKa = 11.84 GGKK45 pKa = 9.31 SVLPRR50 pKa = 11.84 GG51 pKa = 3.7
Molecular weight: 5.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3148
0
3148
940206
37
7398
298.7
33.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.632 ± 0.045
1.455 ± 0.025
5.716 ± 0.036
7.102 ± 0.042
4.046 ± 0.033
7.739 ± 0.045
1.615 ± 0.02
7.237 ± 0.051
6.476 ± 0.037
8.678 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.167 ± 0.029
4.077 ± 0.036
3.587 ± 0.024
3.44 ± 0.024
4.469 ± 0.04
5.552 ± 0.035
5.428 ± 0.063
7.149 ± 0.041
0.811 ± 0.014
3.624 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here