Equid gammaherpesvirus 2 (Equine herpesvirus 2)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3F1B9|A3F1B9_9GAMA E6 OS=Equid gammaherpesvirus 2 OX=12657 GN=E6 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 GILLYY7 pKa = 10.17 GCVWVFLMQFTSTLANQTTVATTTSTTVPATVSTTVPTTVPATVPTTVPATVPTTVPTTVPATVPTTVPTTVPTTVPATVPTTVPTTVPATVPTTVPTTVPATVPTTVTTTVTTTVTTTVTTTVPTTVPATSVASTTQAQTTSNQTTHH155 pKa = 5.61 NTSNPTTTTGGEE167 pKa = 4.0 NEE169 pKa = 4.26 TTIFTSSPITTQNSTEE185 pKa = 4.26 SEE187 pKa = 4.41 VNATEE192 pKa = 4.34 SASTTSTQTSSTPSDD207 pKa = 3.68 LLTFITGNQEE217 pKa = 3.8 TPEE220 pKa = 4.38 PSDD223 pKa = 4.84 DD224 pKa = 5.7 FIDD227 pKa = 4.44 LLPSNISSFFNGGLLLVAAFVLIMLLIVIWNICLCISTHH266 pKa = 5.25 NQLKK270 pKa = 8.57 NHH272 pKa = 6.86 MYY274 pKa = 8.48 QTVPP278 pKa = 3.17
Molecular weight: 28.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.063
IPC2_protein 4.291
IPC_protein 4.126
Toseland 3.973
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.973
Rodwell 3.973
Grimsley 3.897
Solomon 4.075
Lehninger 4.024
Nozaki 4.215
DTASelect 4.329
Thurlkill 4.012
EMBOSS 3.986
Sillero 4.253
Patrickios 0.477
IPC_peptide 4.075
IPC2_peptide 4.228
IPC2.peptide.svr19 4.143
Protein with the highest isoelectric point:
>tr|A0A0B4Q5C5|A0A0B4Q5C5_9GAMA Envelope glycoprotein H OS=Equid gammaherpesvirus 2 OX=12657 GN=ORF22 PE=3 SV=1
MM1 pKa = 7.29 FGVAKK6 pKa = 9.63 PPPSPIPKK14 pKa = 7.77 PTRR17 pKa = 11.84 GRR19 pKa = 11.84 HH20 pKa = 4.86 FGEE23 pKa = 4.97 KK24 pKa = 10.28 YY25 pKa = 9.45 PEE27 pKa = 4.25 SVNFPSRR34 pKa = 11.84 GARR37 pKa = 11.84 QRR39 pKa = 11.84 ASTRR43 pKa = 11.84 HH44 pKa = 5.26 RR45 pKa = 11.84 PSIKK49 pKa = 10.18 EE50 pKa = 3.69 VWEE53 pKa = 4.43 PPAPPGAGMIFWGEE67 pKa = 3.74 NGEE70 pKa = 4.23 NGDD73 pKa = 4.49 FGQSAPSCYY82 pKa = 8.81 EE83 pKa = 3.36 RR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 ARR88 pKa = 11.84 SPGGQGAEE96 pKa = 3.87 NAAPVLRR103 pKa = 11.84 PGPEE107 pKa = 3.31 GRR109 pKa = 11.84 RR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 EE113 pKa = 3.68 SLILLEE119 pKa = 4.38 GDD121 pKa = 3.66 MVRR124 pKa = 11.84 EE125 pKa = 4.31 TATVAPWTVSPLRR138 pKa = 11.84 PEE140 pKa = 4.12 RR141 pKa = 11.84 PKK143 pKa = 11.11 SLLGRR148 pKa = 11.84 FRR150 pKa = 11.84 SKK152 pKa = 9.49 LTVWRR157 pKa = 11.84 EE158 pKa = 3.86 VPRR161 pKa = 11.84 TPGPVAPIPEE171 pKa = 4.6 LPGEE175 pKa = 4.37 ADD177 pKa = 4.15 DD178 pKa = 5.14 PPTRR182 pKa = 11.84 TPPPPPDD189 pKa = 3.65 DD190 pKa = 3.62
Molecular weight: 20.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.326
IPC_protein 10.043
Toseland 10.555
ProMoST 10.248
Dawson 10.643
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.73
Grimsley 10.672
Solomon 10.789
Lehninger 10.76
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.555
EMBOSS 10.965
Sillero 10.57
Patrickios 10.482
IPC_peptide 10.789
IPC2_peptide 9.209
IPC2.peptide.svr19 8.71
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
38132
89
3755
488.9
53.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.164 ± 0.388
2.337 ± 0.183
4.841 ± 0.189
6.202 ± 0.373
4.453 ± 0.22
7.804 ± 0.331
2.31 ± 0.118
3.805 ± 0.18
4.28 ± 0.196
9.714 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.08 ± 0.133
3.422 ± 0.156
6.952 ± 0.773
3.574 ± 0.325
6.068 ± 0.254
7.385 ± 0.225
5.46 ± 0.267
6.947 ± 0.268
1.023 ± 0.071
3.178 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here