Roseospirillum parvum
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3067 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8EYL0|A0A1G8EYL0_9PROT Uncharacterized protein OS=Roseospirillum parvum OX=83401 GN=SAMN05421742_11182 PE=4 SV=1
MM1 pKa = 7.31 GNIVQSEE8 pKa = 4.02 IAANQSNKK16 pKa = 8.85 ATTANDD22 pKa = 3.21 ALAEE26 pKa = 4.05 IDD28 pKa = 4.04 AGMTEE33 pKa = 4.51 LLEE36 pKa = 5.96 IDD38 pKa = 5.29 LSTADD43 pKa = 3.54 ATLTAAQYY51 pKa = 10.63 RR52 pKa = 11.84 GAFTVKK58 pKa = 7.91 TTGNDD63 pKa = 3.47 GTYY66 pKa = 10.66 HH67 pKa = 7.2 LALPAQKK74 pKa = 9.93 RR75 pKa = 11.84 ACGVYY80 pKa = 10.38 NGGTGDD86 pKa = 4.05 LNVTVGATTVTIPAATGYY104 pKa = 10.5 HH105 pKa = 7.4 LYY107 pKa = 10.37 TDD109 pKa = 4.2 GTTDD113 pKa = 3.52 GLISLGGGGGEE124 pKa = 4.32 SDD126 pKa = 5.19 FIGLTDD132 pKa = 3.82 TPTDD136 pKa = 3.52 YY137 pKa = 11.53 TNAAGRR143 pKa = 11.84 AVMVNSTGNALEE155 pKa = 4.08 FATVVEE161 pKa = 4.64 QVGGWFDD168 pKa = 3.72 GAPSASQTVFRR179 pKa = 11.84 VALTDD184 pKa = 3.69 ATDD187 pKa = 4.03 LPADD191 pKa = 3.59 LAGSVFRR198 pKa = 11.84 AGTAPSGGDD207 pKa = 3.15 DD208 pKa = 3.74 VYY210 pKa = 11.61 DD211 pKa = 3.76 VQVAGASIGSVTFADD226 pKa = 4.74 GATSGTASVTATACAAGDD244 pKa = 4.05 VVALIAPTTLYY255 pKa = 11.0 GSADD259 pKa = 3.52 LTVTIKK265 pKa = 10.09 GTLL268 pKa = 3.28
Molecular weight: 26.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.567
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.836
Protein with the highest isoelectric point:
>tr|A0A1G7WQZ5|A0A1G7WQZ5_9PROT Nucleoside diphosphate kinase OS=Roseospirillum parvum OX=83401 GN=ndk PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 10.24 LIRR12 pKa = 11.84 KK13 pKa = 8.58 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.68 ATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.99
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3067
0
3067
1028483
25
5496
335.3
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.501 ± 0.067
0.826 ± 0.013
6.018 ± 0.053
5.856 ± 0.038
3.16 ± 0.025
9.109 ± 0.046
2.273 ± 0.019
4.022 ± 0.034
2.482 ± 0.032
11.29 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.221 ± 0.022
2.098 ± 0.021
6.232 ± 0.048
3.005 ± 0.025
7.732 ± 0.056
4.451 ± 0.031
5.225 ± 0.04
7.327 ± 0.038
1.314 ± 0.018
1.858 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here