Cohnella endophytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494Y241|A0A494Y241_9BACL Sugar ABC transporter permease OS=Cohnella endophytica OX=2419778 GN=D7Z26_13885 PE=3 SV=1
MM1 pKa = 6.97YY2 pKa = 10.6SKK4 pKa = 9.89WCPDD8 pKa = 3.69CEE10 pKa = 5.07QIPCQCNEE18 pKa = 4.8LICNKK23 pKa = 10.06CGEE26 pKa = 4.33RR27 pKa = 11.84NCRR30 pKa = 11.84CKK32 pKa = 10.38QDD34 pKa = 3.0INVTVKK40 pKa = 10.16PKK42 pKa = 10.54QIVNINVPTGATGATGATGATGATGATGATGATGAGATGATGATGATGATGATGVTGVTGATGDD106 pKa = 3.83PGVTGATGATGDD118 pKa = 4.22PGVTGATGATGDD130 pKa = 4.22PGVTGATGATGAGATGATGATGATGATGATGATGPGTGATGPTGPTGATGATGATGDD187 pKa = 4.22PGVTGATGATGATGITGATGATGATGAGATGATGATGATGDD228 pKa = 4.25PGATGATGATGATGATGPGTGATGPTGPTGATGATGDD265 pKa = 4.22PGVTGATGATGATGATGATGATGATGDD292 pKa = 4.22PGVTGATGATGITGATGDD310 pKa = 4.21PGVTGATGATGATGATGITGATGATGATGDD340 pKa = 4.22PGVTGATGATGATGITGATGTTGATGITGATGATGATGDD379 pKa = 4.22PGVTGATGATGATGITGATGATGATGDD406 pKa = 4.22PGVTGATGATGATGITGATGATGATGATGVTGATGATGITGATGATGATGAGAIIPFASGLPTALTTVLGGLLGTTSLVGFGSSVTGVSILTSGDD501 pKa = 2.92IDD503 pKa = 3.72LTGAGGTLLNFSFSVPRR520 pKa = 11.84KK521 pKa = 8.3GTIDD525 pKa = 3.07SMAAYY530 pKa = 9.93FSTTAALSLLGTTITITAQLYY551 pKa = 10.91SSATPNNIFSPIPGAIVTLSPPLTGTLALGTISSGTASGLGITVSQGTRR600 pKa = 11.84LLMVFSANVTSGLSIATTIAGYY622 pKa = 10.93ASAGVDD628 pKa = 3.15ISS630 pKa = 3.48

Molecular weight: 54.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494XRB7|A0A494XRB7_9BACL Uncharacterized protein OS=Cohnella endophytica OX=2419778 GN=D7Z26_16445 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.57NGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.88VLTAA44 pKa = 4.27

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5255

0

5255

1836382

26

6412

349.5

38.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.573 ± 0.046

0.717 ± 0.011

5.306 ± 0.025

6.364 ± 0.044

4.15 ± 0.026

7.659 ± 0.036

1.911 ± 0.017

6.527 ± 0.031

5.206 ± 0.033

9.916 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.017

4.042 ± 0.032

4.073 ± 0.021

3.498 ± 0.017

5.222 ± 0.041

6.671 ± 0.032

5.585 ± 0.044

7.102 ± 0.026

1.362 ± 0.013

3.454 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski