Fertoebacter nigrum
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3609 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7C9K5S6|A0A7C9K5S6_9RHOB Pseudouridine synthase OS=Fertoebacter nigrum OX=2656921 GN=GEU84_15415 PE=3 SV=1
MM1 pKa = 7.27 LVAALMPASMAVAHH15 pKa = 5.78 PHH17 pKa = 5.99 IFIDD21 pKa = 3.53 TGLEE25 pKa = 4.16 VIFDD29 pKa = 3.78 ATGQATALRR38 pKa = 11.84 ITWTYY43 pKa = 11.61 DD44 pKa = 2.96 DD45 pKa = 5.32 FYY47 pKa = 11.36 SLLVMEE53 pKa = 5.54 DD54 pKa = 3.29 RR55 pKa = 11.84 GLDD58 pKa = 3.34 ADD60 pKa = 3.99 FDD62 pKa = 4.5 GVLTADD68 pKa = 3.78 EE69 pKa = 4.07 QAALDD74 pKa = 4.23 GFDD77 pKa = 3.84 MQWVAGFPGDD87 pKa = 3.47 TYY89 pKa = 11.81 ALLGTAPLDD98 pKa = 3.87 LGPPQEE104 pKa = 4.23 WTASYY109 pKa = 11.43 ADD111 pKa = 3.62 GKK113 pKa = 9.09 ITSTHH118 pKa = 5.48 LRR120 pKa = 11.84 RR121 pKa = 11.84 FAAPVAPVADD131 pKa = 3.88 PLVVQVYY138 pKa = 10.85 DD139 pKa = 3.63 PGFYY143 pKa = 9.98 SAYY146 pKa = 9.78 AIAGTPVLTAAPDD159 pKa = 3.48 CSVQVFEE166 pKa = 5.48 PDD168 pKa = 3.6 RR169 pKa = 11.84 EE170 pKa = 4.13 AADD173 pKa = 3.98 QILQAAIAEE182 pKa = 4.47 LAGSVDD188 pKa = 3.31 IEE190 pKa = 4.57 GDD192 pKa = 3.87 FPAVGAAYY200 pKa = 10.3 SEE202 pKa = 4.57 EE203 pKa = 3.88 ARR205 pKa = 11.84 ITCSAGSS212 pKa = 3.42
Molecular weight: 22.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.088
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 0.744
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A7C9GAB9|A0A7C9GAB9_9RHOB Maleylacetoacetate isomerase OS=Fertoebacter nigrum OX=2656921 GN=maiA PE=3 SV=1
MM1 pKa = 7.43 AHH3 pKa = 6.95 PRR5 pKa = 11.84 AQPFPAPGWPRR16 pKa = 11.84 PPARR20 pKa = 11.84 PVPAARR26 pKa = 11.84 PPARR30 pKa = 11.84 PQPAPAPPRR39 pKa = 11.84 HH40 pKa = 6.51 CPRR43 pKa = 11.84 PPQPRR48 pKa = 11.84 IPGSRR53 pKa = 11.84 AFPSSARR60 pKa = 11.84 PRR62 pKa = 11.84 GASRR66 pKa = 11.84 AACPRR71 pKa = 11.84 TSGFPLTPPRR81 pKa = 11.84 GSAHH85 pKa = 4.57 VV86 pKa = 3.4
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.071
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.062
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.071
Sillero 12.559
Patrickios 11.798
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.191
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3609
0
3609
1165447
28
2780
322.9
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.023 ± 0.071
0.878 ± 0.012
5.798 ± 0.034
5.002 ± 0.031
3.639 ± 0.023
9.176 ± 0.046
2.105 ± 0.022
4.724 ± 0.031
2.479 ± 0.03
10.534 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.724 ± 0.018
2.38 ± 0.022
5.458 ± 0.032
3.164 ± 0.017
6.965 ± 0.04
4.573 ± 0.029
5.433 ± 0.03
7.424 ± 0.032
1.431 ± 0.016
2.09 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here