Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CIP 107346 / CN-1) (Corynebacterium nigricans)
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2528 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3PIE5|C3PIE5_CORA7 Uncharacterized protein OS=Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CIP 107346 / CN-1) OX=548476 GN=cauri_2006 PE=4 SV=1
MM1 pKa = 6.2 TTYY4 pKa = 11.05 ARR6 pKa = 11.84 RR7 pKa = 11.84 DD8 pKa = 3.5 DD9 pKa = 4.8 AITRR13 pKa = 11.84 EE14 pKa = 4.14 IIEE17 pKa = 4.18 PLGEE21 pKa = 3.86 YY22 pKa = 10.06 AAEE25 pKa = 4.11 HH26 pKa = 6.54 NIDD29 pKa = 4.63 AIADD33 pKa = 3.69 EE34 pKa = 5.87 LIICDD39 pKa = 4.01 GTGLDD44 pKa = 3.44 PVYY47 pKa = 10.62 YY48 pKa = 9.77 INPDD52 pKa = 3.0 ADD54 pKa = 3.2 FWNIVANNALL64 pKa = 3.62
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|C3PL16|C3PL16_CORA7 Putative transposase OS=Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CIP 107346 / CN-1) OX=548476 GN=cauri_0421 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.5 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2528
0
2528
822074
24
2989
325.2
35.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.767 ± 0.061
0.728 ± 0.015
5.871 ± 0.041
6.755 ± 0.053
3.386 ± 0.032
8.267 ± 0.045
2.153 ± 0.025
4.924 ± 0.034
3.49 ± 0.043
9.529 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.02
2.903 ± 0.034
5.033 ± 0.037
3.305 ± 0.03
6.056 ± 0.043
6.011 ± 0.038
6.04 ± 0.032
7.911 ± 0.042
1.379 ± 0.022
2.273 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here