Zavarzinia compransoris
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317DT32|A0A317DT32_9PROT 2Fe-2S ferredoxin OS=Zavarzinia compransoris OX=1264899 GN=DKG75_22160 PE=4 SV=1
MM1 pKa = 7.3 ATTAAAALSATGFVAAQDD19 pKa = 4.23 AYY21 pKa = 9.82 PVSGDD26 pKa = 3.12 QFGYY30 pKa = 8.17 GTRR33 pKa = 11.84 AVAEE37 pKa = 4.1 TSFNLEE43 pKa = 3.66 IFGGADD49 pKa = 3.56 EE50 pKa = 5.24 NGGTYY55 pKa = 10.47 GGAPSITIPLGDD67 pKa = 3.81 KK68 pKa = 10.87 LGLQVDD74 pKa = 5.01 GVAGFTADD82 pKa = 3.19 EE83 pKa = 4.48 AGFAGGAAQLFYY95 pKa = 10.79 RR96 pKa = 11.84 DD97 pKa = 4.08 PQQGLIGVAAGGYY110 pKa = 7.66 YY111 pKa = 9.88 VEE113 pKa = 5.82 GIKK116 pKa = 10.3 QYY118 pKa = 10.77 SVAGIAEE125 pKa = 4.43 YY126 pKa = 11.21 YY127 pKa = 10.26 LDD129 pKa = 4.83 NITLEE134 pKa = 4.21 GLVGYY139 pKa = 7.47 QTGDD143 pKa = 3.16 VMDD146 pKa = 3.94 SVYY149 pKa = 11.03 GRR151 pKa = 11.84 LGVSIYY157 pKa = 11.14 ANPNLRR163 pKa = 11.84 LGGGVSYY170 pKa = 11.04 SEE172 pKa = 4.05 EE173 pKa = 4.09 TKK175 pKa = 10.49 IGGDD179 pKa = 3.4 IQIEE183 pKa = 4.05 ALLTDD188 pKa = 3.92 VPGLALFATGAFDD201 pKa = 4.03 EE202 pKa = 4.9 YY203 pKa = 10.97 GTMGYY208 pKa = 10.55 GGVRR212 pKa = 11.84 FYY214 pKa = 11.35 FNSGTSLLSTDD225 pKa = 3.49 RR226 pKa = 11.84 TKK228 pKa = 10.72 QAATPSLIDD237 pKa = 2.8 MHH239 pKa = 7.11 RR240 pKa = 11.84 NLGRR244 pKa = 11.84 PNFLTNGGAGFGLRR258 pKa = 11.84 QISLVGAAKK267 pKa = 10.7 NGGDD271 pKa = 4.05 DD272 pKa = 4.29 FGDD275 pKa = 3.63 VTPPPPPPPPPPNPNANCGDD295 pKa = 3.73 GLICGVQDD303 pKa = 3.51 LVGNLTEE310 pKa = 4.19 NTILSPLNDD319 pKa = 5.65 LITGLLDD326 pKa = 4.14 PNSGALSALTGALEE340 pKa = 4.82 DD341 pKa = 4.04 LTSSGGGALGAVTDD355 pKa = 4.47 LVNGLVNTQNQALEE369 pKa = 4.11 PVISGLNSILAQLTGGLTGGLTGGLTGSGATANDD403 pKa = 3.67 GLIDD407 pKa = 3.77 VVQGVVGNLLDD418 pKa = 3.9 GTALEE423 pKa = 4.62 GVGDD427 pKa = 4.09 LVDD430 pKa = 4.7 GLVDD434 pKa = 4.16 PNTGALAGLTGALNDD449 pKa = 4.47 LTSSDD454 pKa = 4.48 AGPLGPVTDD463 pKa = 4.93 LVNGLAGANVGALDD477 pKa = 3.75 PVLDD481 pKa = 4.39 GVHH484 pKa = 7.16 DD485 pKa = 4.76 LLAGLTGGLAGALPGASSGSTDD507 pKa = 3.01 GGLIDD512 pKa = 4.1 TVQGTVDD519 pKa = 3.71 NLLHH523 pKa = 5.53 GTPLEE528 pKa = 4.36 VVSDD532 pKa = 3.92 LVGGLVDD539 pKa = 4.13 PQTGALSALTGQLNNLTAPNAPLGSLTGLVDD570 pKa = 3.99 GLVGAQNGALDD581 pKa = 4.07 PVLDD585 pKa = 4.17 TVNGLLSNNLSSVNLGAAPVVLQNVLPSIPVVGDD619 pKa = 3.65 LLSGLVGAIGGIGGG633 pKa = 3.66
Molecular weight: 62.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.528
IPC_protein 3.579
Toseland 3.338
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.745
Wikipedia 3.554
Rodwell 3.401
Grimsley 3.236
Solomon 3.579
Lehninger 3.541
Nozaki 3.694
DTASelect 3.986
Thurlkill 3.401
EMBOSS 3.554
Sillero 3.706
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.665
Protein with the highest isoelectric point:
>tr|A0A317E066|A0A317E066_9PROT ATP-dependent Clp protease ATP-binding subunit ClpA OS=Zavarzinia compransoris OX=1264899 GN=clpA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.23 QPSALVRR12 pKa = 11.84 KK13 pKa = 8.86 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.76 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTVGGRR28 pKa = 11.84 VVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4405
0
4405
1447416
26
4933
328.6
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.236 ± 0.055
0.781 ± 0.012
5.78 ± 0.028
5.271 ± 0.036
3.636 ± 0.022
9.432 ± 0.051
1.964 ± 0.02
4.77 ± 0.021
2.713 ± 0.028
10.697 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.111 ± 0.016
2.244 ± 0.024
5.59 ± 0.035
2.741 ± 0.021
7.625 ± 0.042
4.404 ± 0.03
5.067 ± 0.041
7.509 ± 0.037
1.288 ± 0.015
2.142 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here