Citrobacter koseri (Citrobacter diversus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A376C3U0|A0A376C3U0_CITKO Uncharacterized protein OS=Citrobacter koseri OX=545 GN=NCTC10810_00130 PE=4 SV=1
MM1 pKa = 7.23SVVAPAVYY9 pKa = 9.81VGTWHH14 pKa = 7.51KK15 pKa = 10.4YY16 pKa = 7.94NCGSIAGRR24 pKa = 11.84WFDD27 pKa = 4.66LATFDD32 pKa = 5.53DD33 pKa = 4.08EE34 pKa = 5.43RR35 pKa = 11.84DD36 pKa = 3.52FFAACRR42 pKa = 11.84SLHH45 pKa = 5.77QDD47 pKa = 3.09EE48 pKa = 5.44ADD50 pKa = 3.82PEE52 pKa = 5.28LMFQDD57 pKa = 4.16YY58 pKa = 11.15EE59 pKa = 4.36GFPGNMASEE68 pKa = 4.31CHH70 pKa = 6.46INWAYY75 pKa = 11.17VEE77 pKa = 4.34GFRR80 pKa = 11.84QARR83 pKa = 11.84DD84 pKa = 3.53EE85 pKa = 4.44GCEE88 pKa = 3.44EE89 pKa = 4.85AYY91 pKa = 10.64RR92 pKa = 11.84LWVDD96 pKa = 3.55DD97 pKa = 3.81TGEE100 pKa = 4.0TDD102 pKa = 4.16FDD104 pKa = 4.06TFRR107 pKa = 11.84DD108 pKa = 3.97AWWGEE113 pKa = 3.61ADD115 pKa = 3.71SEE117 pKa = 4.33EE118 pKa = 4.18AFAVEE123 pKa = 4.69FASDD127 pKa = 3.34TGLLADD133 pKa = 3.86VPEE136 pKa = 4.53TVALYY141 pKa = 10.3FDD143 pKa = 4.08YY144 pKa = 10.8EE145 pKa = 4.24AYY147 pKa = 10.46ARR149 pKa = 11.84DD150 pKa = 3.68LFLDD154 pKa = 3.51SFTFIDD160 pKa = 3.34GHH162 pKa = 4.72VFRR165 pKa = 11.84RR166 pKa = 3.77

Molecular weight: 19.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A381GZA5|A0A381GZA5_CITKO Nitric oxide dioxygenase OS=Citrobacter koseri OX=545 GN=hmp_3 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84KK3 pKa = 9.23LAGNLKK9 pKa = 10.11QSNTLLIFINQIRR22 pKa = 11.84MKK24 pKa = 9.59IGVMFATRR32 pKa = 11.84KK33 pKa = 8.7PLPGVTRR40 pKa = 4.12

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5320

0

5320

1346380

29

1968

253.1

27.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.816 ± 0.04

1.169 ± 0.015

5.167 ± 0.03

5.551 ± 0.035

3.827 ± 0.025

7.468 ± 0.029

2.292 ± 0.018

5.799 ± 0.033

4.128 ± 0.032

10.728 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.02

3.742 ± 0.024

4.479 ± 0.024

4.416 ± 0.029

5.869 ± 0.031

5.883 ± 0.03

5.439 ± 0.025

7.06 ± 0.025

1.553 ± 0.018

2.763 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski