Botryosphaeria dothidea chrysovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Chrysoviridae; Betachrysovirus; Botryosphaeria dothidea chrysovirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023IRJ3|A0A023IRJ3_9VIRU RNA-directed RNA polymerase OS=Botryosphaeria dothidea chrysovirus 1 OX=1405299 GN=RdRp PE=4 SV=1
MM1 pKa = 7.36TSADD5 pKa = 3.51NFFASMLEE13 pKa = 3.82AGAGFGQPDD22 pKa = 3.74AFKK25 pKa = 11.0SQATKK30 pKa = 10.79ADD32 pKa = 3.6RR33 pKa = 11.84GLIDD37 pKa = 4.24FDD39 pKa = 4.05FTGGGQNAKK48 pKa = 10.67SMAQQLLSPGSWTLLQQLSGEE69 pKa = 4.38EE70 pKa = 3.98VHH72 pKa = 6.27QVAVYY77 pKa = 9.96YY78 pKa = 10.6SLEE81 pKa = 4.17RR82 pKa = 11.84EE83 pKa = 4.41GADD86 pKa = 3.37LSSPNLRR93 pKa = 11.84QTTVVYY99 pKa = 9.59PEE101 pKa = 3.93PSSRR105 pKa = 11.84KK106 pKa = 7.52EE107 pKa = 3.43TALACRR113 pKa = 11.84GGASRR118 pKa = 11.84VEE120 pKa = 4.4SIGVDD125 pKa = 3.41VAEE128 pKa = 4.51RR129 pKa = 11.84ALANYY134 pKa = 7.25TRR136 pKa = 11.84SVSTNSSPGAGSAQHH151 pKa = 6.47HH152 pKa = 5.9AHH154 pKa = 6.49LTQMHH159 pKa = 6.06KK160 pKa = 10.08HH161 pKa = 5.19VEE163 pKa = 4.16SLLVVMWLIHH173 pKa = 6.67LDD175 pKa = 3.55VAAGAEE181 pKa = 4.14PVVALDD187 pKa = 3.77QMGVHH192 pKa = 6.99AGLSNVTDD200 pKa = 3.94PADD203 pKa = 3.47EE204 pKa = 4.48CEE206 pKa = 4.1EE207 pKa = 4.11AKK209 pKa = 11.0SIDD212 pKa = 3.48GVRR215 pKa = 11.84LHH217 pKa = 7.14VNSCDD222 pKa = 3.3NTTNALLAAACDD234 pKa = 3.96YY235 pKa = 11.27NAIANLGRR243 pKa = 11.84RR244 pKa = 11.84AEE246 pKa = 4.18RR247 pKa = 11.84YY248 pKa = 8.19KK249 pKa = 9.07WTPSRR254 pKa = 11.84LTLYY258 pKa = 10.15GGLMSASVNVPLTNPDD274 pKa = 2.71ATARR278 pKa = 11.84AINSFGHH285 pKa = 6.71RR286 pKa = 11.84FASPSEE292 pKa = 3.85MSAALEE298 pKa = 4.18TALLLYY304 pKa = 10.5GLEE307 pKa = 4.65DD308 pKa = 3.63GGTRR312 pKa = 11.84VVLDD316 pKa = 3.68VPAPQLHH323 pKa = 6.71EE324 pKa = 4.65DD325 pKa = 3.59SHH327 pKa = 6.98RR328 pKa = 11.84LPMTTSGLARR338 pKa = 11.84TRR340 pKa = 11.84EE341 pKa = 4.12LAGSAIVALSLFLGRR356 pKa = 11.84AYY358 pKa = 9.81MQVAGAVLRR367 pKa = 11.84SAVLTTGAKK376 pKa = 9.51DD377 pKa = 3.13LHH379 pKa = 6.21GAEE382 pKa = 3.94HH383 pKa = 6.77FLRR386 pKa = 11.84RR387 pKa = 11.84NHH389 pKa = 6.59EE390 pKa = 4.24LLQVAAGAHH399 pKa = 6.87LAQTWGQLHH408 pKa = 7.1PNYY411 pKa = 9.33IHH413 pKa = 7.08HH414 pKa = 6.4GTMLHH419 pKa = 5.66VNFVRR424 pKa = 11.84AWQQLGVLHH433 pKa = 7.1ALMAGCVTEE442 pKa = 4.45GSIFEE447 pKa = 4.53TVTNALEE454 pKa = 4.46LGTALDD460 pKa = 4.14TPVTDD465 pKa = 4.42SPSLGQSRR473 pKa = 11.84ADD475 pKa = 4.57EE476 pKa = 4.16MVTWYY481 pKa = 9.95ILSEE485 pKa = 3.97LLRR488 pKa = 11.84DD489 pKa = 4.05GGEE492 pKa = 4.08HH493 pKa = 6.99LNGSRR498 pKa = 11.84AAIKK502 pKa = 10.27RR503 pKa = 11.84SLGASTRR510 pKa = 11.84LHH512 pKa = 6.73AEE514 pKa = 3.84TRR516 pKa = 11.84QYY518 pKa = 11.66AGTKK522 pKa = 9.77ARR524 pKa = 11.84FIISGSGAPLLLRR537 pKa = 11.84MVDD540 pKa = 3.5NSTLALDD547 pKa = 3.98PTPQEE552 pKa = 4.01AAEE555 pKa = 4.25SNAAEE560 pKa = 4.22VDD562 pKa = 3.72TGNDD566 pKa = 3.16TALGQLADD574 pKa = 4.22RR575 pKa = 11.84PALRR579 pKa = 11.84GEE581 pKa = 4.12DD582 pKa = 3.22WVNALVTQRR591 pKa = 11.84GPIASKK597 pKa = 10.39VMQRR601 pKa = 11.84AKK603 pKa = 10.56PPVYY607 pKa = 10.11KK608 pKa = 10.5AGRR611 pKa = 11.84SNGTQIVIEE620 pKa = 4.55RR621 pKa = 11.84LPEE624 pKa = 4.0DD625 pKa = 3.14ARR627 pKa = 11.84LSVGRR632 pKa = 11.84ASRR635 pKa = 11.84LGWGPEE641 pKa = 3.74PTPGYY646 pKa = 11.44GMLCGARR653 pKa = 11.84AITQSLCAVAALRR666 pKa = 11.84NEE668 pKa = 4.13PEE670 pKa = 3.84PLYY673 pKa = 10.29DD674 pKa = 3.61TVRR677 pKa = 11.84TAVQVAMTDD686 pKa = 3.44EE687 pKa = 4.0QRR689 pKa = 11.84QLAEE693 pKa = 4.02VAGVATGIDD702 pKa = 3.97NFTVDD707 pKa = 3.59QLTAGLQQLGDD718 pKa = 3.7YY719 pKa = 9.29RR720 pKa = 11.84LSVVTSVNGQGNVVVHH736 pKa = 6.17GSGNGEE742 pKa = 3.86IVLVASS748 pKa = 4.44

Molecular weight: 79.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023IRJ8|A0A023IRJ8_9VIRU Uncharacterized protein OS=Botryosphaeria dothidea chrysovirus 1 OX=1405299 PE=4 SV=1
MM1 pKa = 7.75SFTRR5 pKa = 11.84DD6 pKa = 3.53PNHH9 pKa = 6.28SWRR12 pKa = 11.84SMAFAGVPVARR23 pKa = 11.84VRR25 pKa = 11.84VTQTVRR31 pKa = 11.84GRR33 pKa = 11.84ADD35 pKa = 3.86LAPADD40 pKa = 4.26TDD42 pKa = 3.39NTAATLEE49 pKa = 3.97AWIDD53 pKa = 3.42HH54 pKa = 5.02QRR56 pKa = 11.84PYY58 pKa = 11.38LLPDD62 pKa = 3.76EE63 pKa = 4.78LAGPAMATGVLASVSEE79 pKa = 4.55VSSADD84 pKa = 3.27HH85 pKa = 7.07SVGLDD90 pKa = 3.16GAARR94 pKa = 11.84IVTGIGYY101 pKa = 8.54NLRR104 pKa = 11.84TKK106 pKa = 7.5TTHH109 pKa = 6.6AVLATGGAHH118 pKa = 5.15MQVTPYY124 pKa = 10.65LDD126 pKa = 3.83PVNVASMTKK135 pKa = 10.03ANSVEE140 pKa = 3.93AFARR144 pKa = 11.84QAEE147 pKa = 4.44IKK149 pKa = 10.04LPRR152 pKa = 11.84VLAPGQPTANQQAQGATYY170 pKa = 10.13NQLEE174 pKa = 4.1ALRR177 pKa = 11.84WEE179 pKa = 4.47IAKK182 pKa = 9.78HH183 pKa = 5.3AAQADD188 pKa = 3.64YY189 pKa = 10.65GARR192 pKa = 11.84LRR194 pKa = 11.84GLARR198 pKa = 11.84VVCHH202 pKa = 5.36THH204 pKa = 5.16VLRR207 pKa = 11.84QAGLKK212 pKa = 10.03AKK214 pKa = 8.37RR215 pKa = 11.84TRR217 pKa = 11.84HH218 pKa = 5.16MGVLAMGSCLTAEE231 pKa = 4.55TPNLAINDD239 pKa = 3.86LNEE242 pKa = 4.13RR243 pKa = 11.84CWLYY247 pKa = 11.33LCDD250 pKa = 4.6EE251 pKa = 4.75LDD253 pKa = 3.25PRR255 pKa = 11.84YY256 pKa = 9.76RR257 pKa = 11.84AFLAMGVRR265 pKa = 11.84GLRR268 pKa = 11.84HH269 pKa = 5.45YY270 pKa = 11.08ADD272 pKa = 4.28PASGTIYY279 pKa = 10.63SEE281 pKa = 4.18LRR283 pKa = 11.84SEE285 pKa = 4.74PEE287 pKa = 3.37NDD289 pKa = 4.03ADD291 pKa = 3.82NQGITFVRR299 pKa = 11.84KK300 pKa = 10.18SGDD303 pKa = 3.07LRR305 pKa = 11.84QPHH308 pKa = 7.64PDD310 pKa = 3.23PADD313 pKa = 3.57YY314 pKa = 11.01EE315 pKa = 4.38RR316 pKa = 11.84VLAAPDD322 pKa = 3.38LCLAYY327 pKa = 10.13YY328 pKa = 9.34YY329 pKa = 10.74AYY331 pKa = 10.32AGSLGIGHH339 pKa = 6.68QATQVLLQTMLGPHH353 pKa = 5.37VWGEE357 pKa = 4.11NARR360 pKa = 11.84LPYY363 pKa = 9.94RR364 pKa = 11.84ASFPKK369 pKa = 10.38LDD371 pKa = 3.48AATYY375 pKa = 10.06LLRR378 pKa = 11.84STDD381 pKa = 3.9PAPPSAVVAHH391 pKa = 6.85FGRR394 pKa = 11.84MLATGPVIAQRR405 pKa = 11.84FLAGLGSVVSGFAGGKK421 pKa = 9.0KK422 pKa = 10.12VSLADD427 pKa = 3.19VMAQVVGVLADD438 pKa = 3.82PDD440 pKa = 3.6QARR443 pKa = 11.84SLLRR447 pKa = 11.84LVWSHH452 pKa = 6.71ASDD455 pKa = 3.78GSAALEE461 pKa = 4.12WVNPFWSGVSEE472 pKa = 5.2AFDD475 pKa = 3.53ACIAAYY481 pKa = 10.04RR482 pKa = 11.84LNAHH486 pKa = 7.52LISQCRR492 pKa = 11.84ATPSQSIRR500 pKa = 11.84PVFNGGVDD508 pKa = 3.32MRR510 pKa = 11.84DD511 pKa = 3.49AIIGSDD517 pKa = 3.96LRR519 pKa = 11.84GEE521 pKa = 3.97PDD523 pKa = 2.91ARR525 pKa = 11.84YY526 pKa = 9.01CEE528 pKa = 4.23SVIYY532 pKa = 9.99QLVAGVPLTCQCEE545 pKa = 4.27PPSGAMYY552 pKa = 10.46GRR554 pKa = 11.84APEE557 pKa = 3.9RR558 pKa = 11.84LGVLRR563 pKa = 11.84SWRR566 pKa = 11.84AVVTWVRR573 pKa = 11.84YY574 pKa = 9.16GLEE577 pKa = 3.79PAKK580 pKa = 10.47RR581 pKa = 11.84LALEE585 pKa = 3.94QEE587 pKa = 4.16EE588 pKa = 4.52PPRR591 pKa = 11.84RR592 pKa = 11.84EE593 pKa = 4.25SKK595 pKa = 10.68SSEE598 pKa = 3.79SSAGVVPALTFTQKK612 pKa = 10.02IPATTTEE619 pKa = 3.96QTKK622 pKa = 9.98PLSDD626 pKa = 4.58PGLQPKK632 pKa = 10.2SLFDD636 pKa = 3.9SAPRR640 pKa = 11.84WQSPATPTVDD650 pKa = 2.98HH651 pKa = 7.27AKK653 pKa = 9.95RR654 pKa = 11.84ASIGSLGSGNRR665 pKa = 11.84RR666 pKa = 11.84DD667 pKa = 4.35FLRR670 pKa = 11.84SPPPAPPPATYY681 pKa = 10.26RR682 pKa = 11.84SPPRR686 pKa = 11.84SDD688 pKa = 3.21APTVVALPAEE698 pKa = 4.74APPAPPSTSDD708 pKa = 3.11EE709 pKa = 3.83MSMRR713 pKa = 11.84GVLPP717 pKa = 3.98

Molecular weight: 77.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3327

717

1116

831.8

91.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.422 ± 0.78

1.503 ± 0.263

5.35 ± 0.206

5.591 ± 0.636

2.765 ± 0.451

7.604 ± 0.534

2.735 ± 0.242

2.825 ± 0.326

2.916 ± 0.411

10.249 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.243

2.916 ± 0.248

5.14 ± 0.895

3.877 ± 0.291

7.304 ± 0.297

6.943 ± 0.207

5.5 ± 0.479

7.815 ± 0.111

1.473 ± 0.136

3.306 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski