Corynebacterium otitidis ATCC 51513
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1846 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0YTM6|K0YTM6_9CORY Uncharacterized protein (Fragment) OS=Corynebacterium otitidis ATCC 51513 OX=883169 GN=HMPREF9719_00211 PE=4 SV=1
MM1 pKa = 7.08 VSAAVGAALLLAGWLGIDD19 pKa = 5.11 LIDD22 pKa = 4.63 GSTEE26 pKa = 3.87 GEE28 pKa = 3.94 PAAGQEE34 pKa = 4.03 PGEE37 pKa = 4.15 LPEE40 pKa = 5.91 CEE42 pKa = 4.57 LATLPEE48 pKa = 4.25 EE49 pKa = 4.25 ADD51 pKa = 3.7 EE52 pKa = 4.45 VVDD55 pKa = 6.16 DD56 pKa = 4.8 ILAGGPFAYY65 pKa = 9.71 PEE67 pKa = 4.09 NDD69 pKa = 3.09 DD70 pKa = 3.81 THH72 pKa = 6.55 FGNYY76 pKa = 8.48 EE77 pKa = 3.71 GRR79 pKa = 11.84 LPEE82 pKa = 5.33 ADD84 pKa = 2.73 GDD86 pKa = 4.14 YY87 pKa = 10.8 YY88 pKa = 11.32 RR89 pKa = 11.84 EE90 pKa = 4.23 FTVEE94 pKa = 4.12 TPGLDD99 pKa = 2.96 HH100 pKa = 7.24 RR101 pKa = 11.84 GPRR104 pKa = 11.84 RR105 pKa = 11.84 IVTGGDD111 pKa = 3.19 SRR113 pKa = 11.84 DD114 pKa = 3.53 PDD116 pKa = 2.99 VWYY119 pKa = 8.44 YY120 pKa = 11.04 TDD122 pKa = 3.53 DD123 pKa = 3.75 HH124 pKa = 7.61 YY125 pKa = 11.78 EE126 pKa = 4.21 SFCEE130 pKa = 5.36 IPDD133 pKa = 3.57 ADD135 pKa = 3.51
Molecular weight: 14.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.795
Dawson 3.668
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.986
Thurlkill 3.516
EMBOSS 3.605
Sillero 3.795
Patrickios 0.693
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|K0YPP0|K0YPP0_9CORY Uncharacterized protein (Fragment) OS=Corynebacterium otitidis ATCC 51513 OX=883169 GN=HMPREF9719_01606 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.29 KK15 pKa = 9.76 KK16 pKa = 8.72 HH17 pKa = 5.56 RR18 pKa = 11.84 KK19 pKa = 5.56 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1846
0
1846
610217
22
3030
330.6
35.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.315 ± 0.104
0.571 ± 0.012
6.231 ± 0.053
7.422 ± 0.071
2.962 ± 0.036
9.482 ± 0.055
1.833 ± 0.023
4.126 ± 0.038
2.627 ± 0.049
9.833 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.631 ± 0.022
2.208 ± 0.032
5.594 ± 0.042
2.614 ± 0.034
7.451 ± 0.057
5.299 ± 0.032
5.26 ± 0.038
8.313 ± 0.057
1.269 ± 0.023
1.959 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here