Torque teno midi virus 1 (isolate MD1-073)

Taxonomy: Viruses; Anelloviridae; Gammatorquevirus; Torque teno midi virus 1

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A4GZ95-3|ORF3-3_TTVG1 Isoform of A4GZ95 Isoform 3 of Uncharacterized ORF3 protein OS=Torque teno midi virus 1 (isolate MD1-073) OX=766184 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.26QNLSAKK7 pKa = 10.27DD8 pKa = 3.79FYY10 pKa = 11.44KK11 pKa = 10.12PCRR14 pKa = 11.84YY15 pKa = 9.83NCEE18 pKa = 4.43TKK20 pKa = 10.66NQMWMSGIADD30 pKa = 4.07SHH32 pKa = 7.27DD33 pKa = 3.43SWCDD37 pKa = 2.92CDD39 pKa = 4.31TPFAHH44 pKa = 7.4LLASIFPPGHH54 pKa = 5.8TDD56 pKa = 3.06RR57 pKa = 11.84TRR59 pKa = 11.84TIQEE63 pKa = 3.37ILTRR67 pKa = 11.84DD68 pKa = 3.56FRR70 pKa = 11.84KK71 pKa = 7.97TCLSGGADD79 pKa = 3.35ATNSGMAEE87 pKa = 3.98TIEE90 pKa = 4.29EE91 pKa = 4.18KK92 pKa = 10.79RR93 pKa = 11.84EE94 pKa = 3.83DD95 pKa = 3.6FQKK98 pKa = 10.98EE99 pKa = 3.9EE100 pKa = 4.65KK101 pKa = 10.43EE102 pKa = 4.63DD103 pKa = 3.62FTEE106 pKa = 4.14EE107 pKa = 4.13QNIEE111 pKa = 4.1DD112 pKa = 4.42LLAAVADD119 pKa = 3.93AEE121 pKa = 4.48GRR123 pKa = 3.7

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A4GZ95-2|ORF3-2_TTVG1 Isoform of A4GZ95 Isoform 2 of Uncharacterized ORF3 protein OS=Torque teno midi virus 1 (isolate MD1-073) OX=766184 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.35PFWWGRR7 pKa = 11.84RR8 pKa = 11.84NKK10 pKa = 9.48FWYY13 pKa = 8.69GRR15 pKa = 11.84NYY17 pKa = 9.7RR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 9.37KK21 pKa = 9.86RR22 pKa = 11.84RR23 pKa = 11.84FPKK26 pKa = 8.58RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 8.07RR30 pKa = 11.84RR31 pKa = 11.84FYY33 pKa = 10.69RR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 8.83YY38 pKa = 9.96RR39 pKa = 11.84RR40 pKa = 11.84PARR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 8.95VRR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 9.75KK56 pKa = 8.97KK57 pKa = 8.65TLIVRR62 pKa = 11.84QWQPDD67 pKa = 4.04SIVLCKK73 pKa = 10.36IKK75 pKa = 10.61GYY77 pKa = 11.1DD78 pKa = 3.55SIIWGAEE85 pKa = 3.73GTQFQCSTHH94 pKa = 5.79EE95 pKa = 4.03MYY97 pKa = 10.47EE98 pKa = 4.13YY99 pKa = 10.48TRR101 pKa = 11.84QKK103 pKa = 10.77YY104 pKa = 9.48PGGGGFGVQLYY115 pKa = 10.08SLEE118 pKa = 3.98YY119 pKa = 10.66LYY121 pKa = 10.71DD122 pKa = 3.33QWKK125 pKa = 9.45LRR127 pKa = 11.84NNIWTKK133 pKa = 9.91TNQLKK138 pKa = 10.4DD139 pKa = 3.13LCRR142 pKa = 11.84YY143 pKa = 7.35LKK145 pKa = 10.71CVMTFYY151 pKa = 10.74RR152 pKa = 11.84HH153 pKa = 4.19QHH155 pKa = 4.83IDD157 pKa = 3.36FVIVYY162 pKa = 7.96EE163 pKa = 4.09RR164 pKa = 11.84QPPFEE169 pKa = 4.57IDD171 pKa = 2.54KK172 pKa = 8.94LTYY175 pKa = 9.08MKK177 pKa = 9.9YY178 pKa = 9.98HH179 pKa = 7.61PYY181 pKa = 9.72MLLQRR186 pKa = 11.84KK187 pKa = 8.47HH188 pKa = 6.78KK189 pKa = 10.31IILPSQTTNPRR200 pKa = 11.84GKK202 pKa = 10.11LKK204 pKa = 10.41KK205 pKa = 9.93KK206 pKa = 9.43KK207 pKa = 8.62TIKK210 pKa = 10.02PPKK213 pKa = 9.77QMLSKK218 pKa = 10.27WFFQQQFAKK227 pKa = 10.74YY228 pKa = 9.53DD229 pKa = 3.81LLLIAAAACSLRR241 pKa = 11.84YY242 pKa = 9.21PRR244 pKa = 11.84IGCCNEE250 pKa = 3.06NRR252 pKa = 11.84MITLYY257 pKa = 10.84CLNTKK262 pKa = 9.85FYY264 pKa = 11.01QDD266 pKa = 4.13TEE268 pKa = 4.02WGTTKK273 pKa = 10.3QAPHH277 pKa = 6.1YY278 pKa = 7.35FKK280 pKa = 10.7PYY282 pKa = 8.64ATINKK287 pKa = 9.79SMIFVSNYY295 pKa = 9.18GGKK298 pKa = 8.07KK299 pKa = 7.44TEE301 pKa = 3.99YY302 pKa = 10.89NIGQWIEE309 pKa = 3.58TDD311 pKa = 3.3IPGEE315 pKa = 4.3GNLARR320 pKa = 11.84YY321 pKa = 8.24YY322 pKa = 10.77RR323 pKa = 11.84SISKK327 pKa = 10.27EE328 pKa = 3.48GGYY331 pKa = 10.15FSPKK335 pKa = 9.55ILQAYY340 pKa = 4.93QTKK343 pKa = 10.1VKK345 pKa = 10.72SVDD348 pKa = 3.67YY349 pKa = 10.41KK350 pKa = 10.64PLPIVLGRR358 pKa = 11.84YY359 pKa = 8.8NPAIDD364 pKa = 4.27DD365 pKa = 4.0GKK367 pKa = 11.26GNKK370 pKa = 9.14IYY372 pKa = 10.58LQTIMNGHH380 pKa = 6.96WGLPQKK386 pKa = 9.76TPDD389 pKa = 3.82YY390 pKa = 10.32IIEE393 pKa = 4.32EE394 pKa = 4.3VPLWLGFWGYY404 pKa = 11.6YY405 pKa = 10.23NYY407 pKa = 10.88LKK409 pKa = 9.02QTRR412 pKa = 11.84TEE414 pKa = 4.57AIFPLHH420 pKa = 5.94MFVVQSKK427 pKa = 9.98YY428 pKa = 10.45IQTQQTEE435 pKa = 4.42TPNNFWAFIDD445 pKa = 3.53NSFIQGKK452 pKa = 8.6NPWDD456 pKa = 3.57SVITYY461 pKa = 9.81SEE463 pKa = 3.89QKK465 pKa = 10.35LWFPTVAWQLKK476 pKa = 7.86TINAICEE483 pKa = 4.05SGPYY487 pKa = 9.18VPKK490 pKa = 10.56LDD492 pKa = 3.54NQTYY496 pKa = 7.58STWEE500 pKa = 3.92LATHH504 pKa = 6.28YY505 pKa = 10.55SFHH508 pKa = 6.68FKK510 pKa = 9.82WGGPQISDD518 pKa = 4.03QPVEE522 pKa = 4.62DD523 pKa = 4.56PGNKK527 pKa = 9.29NKK529 pKa = 10.3YY530 pKa = 9.42DD531 pKa = 3.52VPDD534 pKa = 4.39TIKK537 pKa = 10.76EE538 pKa = 3.83ALQIVNPAKK547 pKa = 10.76NIAATMFHH555 pKa = 6.8DD556 pKa = 3.09WDD558 pKa = 3.76YY559 pKa = 11.74RR560 pKa = 11.84RR561 pKa = 11.84GCITSTAIKK570 pKa = 10.3RR571 pKa = 11.84MQQNLPTDD579 pKa = 3.53SSLEE583 pKa = 3.99SDD585 pKa = 4.22SDD587 pKa = 4.33SEE589 pKa = 4.06PAPKK593 pKa = 9.93KK594 pKa = 10.32KK595 pKa = 10.13RR596 pKa = 11.84LLPVLHH602 pKa = 7.34DD603 pKa = 3.94PQKK606 pKa = 9.96KK607 pKa = 6.76TEE609 pKa = 4.79KK610 pKa = 10.3INQCLLSLCEE620 pKa = 4.27EE621 pKa = 4.61STCQEE626 pKa = 3.95QEE628 pKa = 3.98TEE630 pKa = 4.1EE631 pKa = 4.7NILKK635 pKa = 10.58LIQQQQQQQQKK646 pKa = 9.55LKK648 pKa = 10.67HH649 pKa = 5.85NLLVLIKK656 pKa = 10.17DD657 pKa = 4.01LKK659 pKa = 9.88VKK661 pKa = 10.23QRR663 pKa = 11.84LLQLQTGVLEE673 pKa = 4.3

Molecular weight: 80.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

2

4

1316

123

673

329.0

38.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.395 ± 1.477

2.356 ± 0.423

5.091 ± 0.988

6.155 ± 1.251

3.951 ± 0.496

4.863 ± 0.489

2.356 ± 0.322

5.243 ± 0.943

9.043 ± 0.874

7.447 ± 0.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.315

4.559 ± 0.084

5.319 ± 0.917

6.687 ± 0.828

6.383 ± 0.255

6.231 ± 1.693

8.131 ± 1.143

2.356 ± 0.82

2.052 ± 0.599

4.179 ± 1.473
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski