Amino acid dipepetide frequency for Tieghemostelium lacteum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.778AlaAla: 1.778 ± 0.025	0.55AlaCys: 0.55 ± 0.011	1.623AlaAsp: 1.623 ± 0.021	1.692AlaGlu: 1.692 ± 0.022	1.616AlaPhe: 1.616 ± 0.017	1.798AlaGly: 1.798 ± 0.026	0.666AlaHis: 0.666 ± 0.012	3.119AlaIle: 3.119 ± 0.025	2.441AlaLys: 2.441 ± 0.027	3.581AlaLeu: 3.581 ± 0.033	0.825AlaMet: 0.825 ± 0.012	2.284AlaAsn: 2.284 ± 0.022	1.402AlaPro: 1.402 ± 0.016	1.515AlaGln: 1.515 ± 0.02	1.115AlaArg: 1.115 ± 0.016	3.161AlaSer: 3.161 ± 0.028	2.717AlaThr: 2.717 ± 0.075	1.98AlaVal: 1.98 ± 0.022	0.289AlaTrp: 0.289 ± 0.007	1.241AlaTyr: 1.241 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	0.519CysAla: 0.519 ± 0.011	0.241CysCys: 0.241 ± 0.008	0.778CysAsp: 0.778 ± 0.021	0.669CysGlu: 0.669 ± 0.01	0.677CysPhe: 0.677 ± 0.013	0.795CysGly: 0.795 ± 0.016	0.3CysHis: 0.3 ± 0.008	1.087CysIle: 1.087 ± 0.014	1.143CysLys: 1.143 ± 0.017	1.324CysLeu: 1.324 ± 0.018	0.23CysMet: 0.23 ± 0.005	0.974CysAsn: 0.974 ± 0.016	0.583CysPro: 0.583 ± 0.014	0.742CysGln: 0.742 ± 0.014	0.49CysArg: 0.49 ± 0.009	1.235CysSer: 1.235 ± 0.017	0.921CysThr: 0.921 ± 0.019	0.745CysVal: 0.745 ± 0.014	0.139CysTrp: 0.139 ± 0.005	0.585CysTyr: 0.585 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	1.641AspAla: 1.641 ± 0.022	0.796AspCys: 0.796 ± 0.013	3.758AspAsp: 3.758 ± 0.046	3.604AspGlu: 3.604 ± 0.035	2.695AspPhe: 2.695 ± 0.021	2.482AspGly: 2.482 ± 0.026	1.146AspHis: 1.146 ± 0.015	4.859AspIle: 4.859 ± 0.031	3.803AspLys: 3.803 ± 0.034	5.109AspLeu: 5.109 ± 0.034	1.001AspMet: 1.001 ± 0.012	3.648AspAsn: 3.648 ± 0.03	2.026AspPro: 2.026 ± 0.019	2.726AspGln: 2.726 ± 0.029	1.81AspArg: 1.81 ± 0.023	4.712AspSer: 4.712 ± 0.032	2.707AspThr: 2.707 ± 0.023	2.346AspVal: 2.346 ± 0.019	0.505AspTrp: 0.505 ± 0.01	2.279AspTyr: 2.279 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	1.927GluAla: 1.927 ± 0.024	0.821GluCys: 0.821 ± 0.013	3.386GluAsp: 3.386 ± 0.034	4.799GluGlu: 4.799 ± 0.067	2.854GluPhe: 2.854 ± 0.023	2.042GluGly: 2.042 ± 0.02	0.986GluHis: 0.986 ± 0.014	5.08GluIle: 5.08 ± 0.034	5.171GluLys: 5.171 ± 0.045	5.298GluLeu: 5.298 ± 0.04	1.375GluMet: 1.375 ± 0.016	4.179GluAsn: 4.179 ± 0.028	1.571GluPro: 1.571 ± 0.017	2.561GluGln: 2.561 ± 0.029	2.045GluArg: 2.045 ± 0.03	4.689GluSer: 4.689 ± 0.032	3.033GluThr: 3.033 ± 0.025	2.559GluVal: 2.559 ± 0.023	0.522GluTrp: 0.522 ± 0.01	2.532GluTyr: 2.532 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	1.546PheAla: 1.546 ± 0.019	0.643PheCys: 0.643 ± 0.012	2.638PheAsp: 2.638 ± 0.026	2.623PheGlu: 2.623 ± 0.024	1.974PhePhe: 1.974 ± 0.023	2.442PheGly: 2.442 ± 0.026	0.963PheHis: 0.963 ± 0.014	3.859PheIle: 3.859 ± 0.03	3.56PheLys: 3.56 ± 0.031	4.022PheLeu: 4.022 ± 0.035	0.793PheMet: 0.793 ± 0.012	3.51PheAsn: 3.51 ± 0.032	1.682PhePro: 1.682 ± 0.019	2.3PheGln: 2.3 ± 0.022	1.454PheArg: 1.454 ± 0.018	4.143PheSer: 4.143 ± 0.033	2.847PheThr: 2.847 ± 0.027	2.237PheVal: 2.237 ± 0.021	0.393PheTrp: 0.393 ± 0.009	2.105PheTyr: 2.105 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	1.908GlyAla: 1.908 ± 0.023	0.772GlyCys: 0.772 ± 0.018	2.477GlyAsp: 2.477 ± 0.024	2.145GlyGlu: 2.145 ± 0.02	2.229GlyPhe: 2.229 ± 0.025	3.341GlyGly: 3.341 ± 0.047	0.949GlyHis: 0.949 ± 0.016	3.516GlyIle: 3.516 ± 0.032	3.01GlyLys: 3.01 ± 0.029	3.679GlyLeu: 3.679 ± 0.028	0.855GlyMet: 0.855 ± 0.016	3.029GlyAsn: 3.029 ± 0.028	1.307GlyPro: 1.307 ± 0.021	1.787GlyGln: 1.787 ± 0.028	1.431GlyArg: 1.431 ± 0.017	4.391GlySer: 4.391 ± 0.037	2.831GlyThr: 2.831 ± 0.028	2.653GlyVal: 2.653 ± 0.027	0.452GlyTrp: 0.452 ± 0.01	2.039GlyTyr: 2.039 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	0.598HisAla: 0.598 ± 0.012	0.392HisCys: 0.392 ± 0.009	0.829HisAsp: 0.829 ± 0.013	0.859HisGlu: 0.859 ± 0.015	1.122HisPhe: 1.122 ± 0.015	0.84HisGly: 0.84 ± 0.015	0.965HisHis: 0.965 ± 0.025	1.463HisIle: 1.463 ± 0.014	1.27HisLys: 1.27 ± 0.018	2.397HisLeu: 2.397 ± 0.024	0.357HisMet: 0.357 ± 0.009	1.316HisAsn: 1.316 ± 0.017	1.055HisPro: 1.055 ± 0.014	1.382HisGln: 1.382 ± 0.02	0.771HisArg: 0.771 ± 0.012	1.933HisSer: 1.933 ± 0.022	1.024HisThr: 1.024 ± 0.017	0.867HisVal: 0.867 ± 0.013	0.229HisTrp: 0.229 ± 0.006	0.994HisTyr: 0.994 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	2.942IleAla: 2.942 ± 0.026	1.126IleCys: 1.126 ± 0.017	4.894IleAsp: 4.894 ± 0.032	5.021IleGlu: 5.021 ± 0.033	3.501IlePhe: 3.501 ± 0.03	3.574IleGly: 3.574 ± 0.031	1.731IleHis: 1.731 ± 0.021	5.89IleIle: 5.89 ± 0.043	5.926IleLys: 5.926 ± 0.032	7.439IleLeu: 7.439 ± 0.047	1.258IleMet: 1.258 ± 0.014	5.527IleAsn: 5.527 ± 0.039	3.88IlePro: 3.88 ± 0.033	4.214IleGln: 4.214 ± 0.033	2.573IleArg: 2.573 ± 0.022	7.43IleSer: 7.43 ± 0.039	4.859IleThr: 4.859 ± 0.035	4.142IleVal: 4.142 ± 0.028	0.596IleTrp: 0.596 ± 0.013	3.128IleTyr: 3.128 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.451LysAla: 2.451 ± 0.029	1.029LysCys: 1.029 ± 0.019	4.081LysAsp: 4.081 ± 0.034	5.412LysGlu: 5.412 ± 0.048	3.185LysPhe: 3.185 ± 0.03	2.566LysGly: 2.566 ± 0.024	1.443LysHis: 1.443 ± 0.014	5.371LysIle: 5.371 ± 0.035	6.719LysLys: 6.719 ± 0.066	7.247LysLeu: 7.247 ± 0.043	1.572LysMet: 1.572 ± 0.019	4.608LysAsn: 4.608 ± 0.029	3.069LysPro: 3.069 ± 0.034	3.349LysGln: 3.349 ± 0.027	2.709LysArg: 2.709 ± 0.024	6.942LysSer: 6.942 ± 0.042	3.894LysThr: 3.894 ± 0.033	3.726LysVal: 3.726 ± 0.025	0.654LysTrp: 0.654 ± 0.011	3.066LysTyr: 3.066 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	3.609LeuAla: 3.609 ± 0.031	1.246LeuCys: 1.246 ± 0.017	5.236LeuAsp: 5.236 ± 0.035	6.104LeuGlu: 6.104 ± 0.049	4.472LeuPhe: 4.472 ± 0.038	3.725LeuGly: 3.725 ± 0.03	1.9LeuHis: 1.9 ± 0.022	6.922LeuIle: 6.922 ± 0.047	8.01LeuLys: 8.01 ± 0.05	9.363LeuLeu: 9.363 ± 0.073	1.802LeuMet: 1.802 ± 0.02	6.805LeuAsn: 6.805 ± 0.044	4.347LeuPro: 4.347 ± 0.033	4.462LeuGln: 4.462 ± 0.032	2.838LeuArg: 2.838 ± 0.027	8.794LeuSer: 8.794 ± 0.046	5.3LeuThr: 5.3 ± 0.036	5.403LeuVal: 5.403 ± 0.035	0.72LeuTrp: 0.72 ± 0.013	3.609LeuTyr: 3.609 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	0.946MetAla: 0.946 ± 0.015	0.219MetCys: 0.219 ± 0.007	1.344MetAsp: 1.344 ± 0.016	1.397MetGlu: 1.397 ± 0.015	0.779MetPhe: 0.779 ± 0.013	0.945MetGly: 0.945 ± 0.014	0.233MetHis: 0.233 ± 0.006	1.557MetIle: 1.557 ± 0.018	1.573MetLys: 1.573 ± 0.017	1.462MetLeu: 1.462 ± 0.019	0.46MetMet: 0.46 ± 0.01	1.562MetAsn: 1.562 ± 0.024	0.54MetPro: 0.54 ± 0.01	0.574MetGln: 0.574 ± 0.01	0.488MetArg: 0.488 ± 0.011	1.679MetSer: 1.679 ± 0.016	1.075MetThr: 1.075 ± 0.015	1.314MetVal: 1.314 ± 0.013	0.147MetTrp: 0.147 ± 0.005	0.598MetTyr: 0.598 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.269AsnAla: 2.269 ± 0.021	0.984AsnCys: 0.984 ± 0.017	3.581AsnAsp: 3.581 ± 0.028	3.784AsnGlu: 3.784 ± 0.03	2.927AsnPhe: 2.927 ± 0.028	3.571AsnGly: 3.571 ± 0.033	1.644AsnHis: 1.644 ± 0.018	6.365AsnIle: 6.365 ± 0.045	4.121AsnLys: 4.121 ± 0.03	6.648AsnLeu: 6.648 ± 0.041	1.182AsnMet: 1.182 ± 0.017	11.102AsnAsn: 11.102 ± 0.138	2.844AsnPro: 2.844 ± 0.025	4.191AsnGln: 4.191 ± 0.038	2.424AsnArg: 2.424 ± 0.023	8.09AsnSer: 8.09 ± 0.06	4.551AsnThr: 4.551 ± 0.038	3.238AsnVal: 3.238 ± 0.025	0.567AsnTrp: 0.567 ± 0.011	2.858AsnTyr: 2.858 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	1.142ProAla: 1.142 ± 0.016	0.393ProCys: 0.393 ± 0.009	1.789ProAsp: 1.789 ± 0.017	2.095ProGlu: 2.095 ± 0.018	1.856ProPhe: 1.856 ± 0.019	1.543ProGly: 1.543 ± 0.025	0.86ProHis: 0.86 ± 0.013	3.761ProIle: 3.761 ± 0.031	3.026ProLys: 3.026 ± 0.027	4.023ProLeu: 4.023 ± 0.035	0.861ProMet: 0.861 ± 0.014	3.27ProAsn: 3.27 ± 0.029	3.277ProPro: 3.277 ± 0.055	2.448ProGln: 2.448 ± 0.039	1.067ProArg: 1.067 ± 0.014	4.26ProSer: 4.26 ± 0.033	3.826ProThr: 3.826 ± 0.034	2.447ProVal: 2.447 ± 0.037	0.302ProTrp: 0.302 ± 0.01	1.534ProTyr: 1.534 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	1.565GlnAla: 1.565 ± 0.021	0.756GlnCys: 0.756 ± 0.013	2.165GlnAsp: 2.165 ± 0.021	2.715GlnGlu: 2.715 ± 0.027	2.314GlnPhe: 2.314 ± 0.025	1.604GlnGly: 1.604 ± 0.02	1.22GlnHis: 1.22 ± 0.018	3.524GlnIle: 3.524 ± 0.028	3.252GlnLys: 3.252 ± 0.03	5.16GlnLeu: 5.16 ± 0.042	1.058GlnMet: 1.058 ± 0.013	3.424GlnAsn: 3.424 ± 0.031	2.638GlnPro: 2.638 ± 0.043	6.923GlnGln: 6.923 ± 0.155	1.514GlnArg: 1.514 ± 0.022	4.551GlnSer: 4.551 ± 0.034	2.65GlnThr: 2.65 ± 0.029	2.389GlnVal: 2.389 ± 0.023	0.477GlnTrp: 0.477 ± 0.01	2.135GlnTyr: 2.135 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	1.203ArgAla: 1.203 ± 0.017	0.434ArgCys: 0.434 ± 0.009	2.087ArgAsp: 2.087 ± 0.027	2.115ArgGlu: 2.115 ± 0.023	1.674ArgPhe: 1.674 ± 0.02	1.457ArgGly: 1.457 ± 0.017	0.576ArgHis: 0.576 ± 0.009	2.498ArgIle: 2.498 ± 0.022	2.378ArgLys: 2.378 ± 0.025	3.031ArgLeu: 3.031 ± 0.034	0.652ArgMet: 0.652 ± 0.011	2.052ArgAsn: 2.052 ± 0.021	1.029ArgPro: 1.029 ± 0.015	1.152ArgGln: 1.152 ± 0.013	1.071ArgArg: 1.071 ± 0.016	2.494ArgSer: 2.494 ± 0.026	1.396ArgThr: 1.396 ± 0.017	1.877ArgVal: 1.877 ± 0.019	0.296ArgTrp: 0.296 ± 0.008	1.511ArgTyr: 1.511 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	3.188SerAla: 3.188 ± 0.029	1.149SerCys: 1.149 ± 0.021	4.528SerAsp: 4.528 ± 0.035	4.23SerGlu: 4.23 ± 0.029	4.107SerPhe: 4.107 ± 0.032	4.547SerGly: 4.547 ± 0.039	1.777SerHis: 1.777 ± 0.019	7.852SerIle: 7.852 ± 0.045	6.784SerLys: 6.784 ± 0.041	9.025SerLeu: 9.025 ± 0.053	1.706SerMet: 1.706 ± 0.02	8.157SerAsn: 8.157 ± 0.05	4.612SerPro: 4.612 ± 0.052	4.223SerGln: 4.223 ± 0.034	2.486SerArg: 2.486 ± 0.031	11.701SerSer: 11.701 ± 0.114	7.403SerThr: 7.403 ± 0.057	4.493SerVal: 4.493 ± 0.03	0.617SerTrp: 0.617 ± 0.01	2.98SerTyr: 2.98 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	2.522ThrAla: 2.522 ± 0.06	0.894ThrCys: 0.894 ± 0.017	2.707ThrAsp: 2.707 ± 0.028	2.779ThrGlu: 2.779 ± 0.025	2.795ThrPhe: 2.795 ± 0.025	2.793ThrGly: 2.793 ± 0.04	1.114ThrHis: 1.114 ± 0.015	5.29ThrIle: 5.29 ± 0.032	4.025ThrLys: 4.025 ± 0.031	6.162ThrLeu: 6.162 ± 0.039	1.084ThrMet: 1.084 ± 0.014	4.36ThrAsn: 4.36 ± 0.036	3.784ThrPro: 3.784 ± 0.039	2.543ThrGln: 2.543 ± 0.029	1.514ThrArg: 1.514 ± 0.018	6.784ThrSer: 6.784 ± 0.063	7.074ThrThr: 7.074 ± 0.086	3.267ThrVal: 3.267 ± 0.028	0.414ThrTrp: 0.414 ± 0.009	1.927ThrTyr: 1.927 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	2.068ValAla: 2.068 ± 0.022	0.807ValCys: 0.807 ± 0.013	3.167ValAsp: 3.167 ± 0.025	3.073ValGlu: 3.073 ± 0.025	2.368ValPhe: 2.368 ± 0.022	2.479ValGly: 2.479 ± 0.026	1.031ValHis: 1.031 ± 0.014	3.905ValIle: 3.905 ± 0.029	3.529ValLys: 3.529 ± 0.031	4.725ValLeu: 4.725 ± 0.033	0.953ValMet: 0.953 ± 0.015	3.639ValAsn: 3.639 ± 0.03	2.291ValPro: 2.291 ± 0.023	2.238ValGln: 2.238 ± 0.021	1.419ValArg: 1.419 ± 0.017	4.381ValSer: 4.381 ± 0.032	3.175ValThr: 3.175 ± 0.041	3.139ValVal: 3.139 ± 0.027	0.444ValTrp: 0.444 ± 0.011	2.096ValTyr: 2.096 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	0.335TrpAla: 0.335 ± 0.008	0.152TrpCys: 0.152 ± 0.006	0.571TrpAsp: 0.571 ± 0.013	0.464TrpGlu: 0.464 ± 0.009	0.409TrpPhe: 0.409 ± 0.009	0.397TrpGly: 0.397 ± 0.01	0.12TrpHis: 0.12 ± 0.005	0.652TrpIle: 0.652 ± 0.013	0.717TrpLys: 0.717 ± 0.013	0.7TrpLeu: 0.7 ± 0.014	0.187TrpMet: 0.187 ± 0.006	0.636TrpAsn: 0.636 ± 0.011	0.206TrpPro: 0.206 ± 0.006	0.229TrpGln: 0.229 ± 0.007	0.355TrpArg: 0.355 ± 0.008	0.686TrpSer: 0.686 ± 0.012	0.448TrpThr: 0.448 ± 0.01	0.519TrpVal: 0.519 ± 0.011	0.099TrpTrp: 0.099 ± 0.005	0.335TrpTyr: 0.335 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.237TyrAla: 1.237 ± 0.016	0.773TyrCys: 0.773 ± 0.024	2.016TyrAsp: 2.016 ± 0.02	1.85TyrGlu: 1.85 ± 0.018	2.183TyrPhe: 2.183 ± 0.019	1.858TyrGly: 1.858 ± 0.021	1.039TyrHis: 1.039 ± 0.015	3.078TyrIle: 3.078 ± 0.028	2.634TyrLys: 2.634 ± 0.023	4.274TyrLeu: 4.274 ± 0.035	0.682TyrMet: 0.682 ± 0.011	2.858TyrAsn: 2.858 ± 0.024	1.638TyrPro: 1.638 ± 0.023	2.474TyrGln: 2.474 ± 0.022	1.409TyrArg: 1.409 ± 0.017	3.51TyrSer: 3.51 ± 0.031	2.097TyrThr: 2.097 ± 0.021	1.585TyrVal: 1.585 ± 0.019	0.357TyrTrp: 0.357 ± 0.009	2.009TyrTyr: 2.009 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10208 proteins (5820820 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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