Candidatus Phytoplasma pini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrXXI (Pine shoot proliferation group)

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559KJU8|A0A559KJU8_9MOLU Poly(A)polymerase OS=Candidatus Phytoplasma pini OX=267362 GN=pcnB PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.2KK3 pKa = 10.27KK4 pKa = 10.69NSIQNDD10 pKa = 3.75LNIVNSISMLEE21 pKa = 3.87LSYY24 pKa = 11.07QILEE28 pKa = 4.26NNRR31 pKa = 11.84DD32 pKa = 3.49PMSIHH37 pKa = 5.84QLIKK41 pKa = 10.57KK42 pKa = 8.28VLEE45 pKa = 3.92MKK47 pKa = 10.55KK48 pKa = 9.89IDD50 pKa = 3.59ISDD53 pKa = 3.67EE54 pKa = 4.1NKK56 pKa = 9.97ISQLYY61 pKa = 10.33LDD63 pKa = 5.42IILSGRR69 pKa = 11.84FVFHH73 pKa = 7.37GNDD76 pKa = 2.74LWSIKK81 pKa = 10.23KK82 pKa = 10.23NNLSLWDD89 pKa = 3.54QEE91 pKa = 4.53YY92 pKa = 10.57FILDD96 pKa = 3.91TIEE99 pKa = 4.14EE100 pKa = 4.1KK101 pKa = 10.9LINDD105 pKa = 3.81EE106 pKa = 4.88EE107 pKa = 4.34ILDD110 pKa = 3.73FDD112 pKa = 4.93EE113 pKa = 5.56FVLRR117 pKa = 11.84NKK119 pKa = 9.92KK120 pKa = 10.47KK121 pKa = 10.21KK122 pKa = 10.65DD123 pKa = 3.31NTEE126 pKa = 3.93EE127 pKa = 4.0EE128 pKa = 3.83KK129 pKa = 10.88DD130 pKa = 3.59ANNFEE135 pKa = 4.74HH136 pKa = 7.58LDD138 pKa = 3.55EE139 pKa = 5.45ALEE142 pKa = 4.47DD143 pKa = 3.91NEE145 pKa = 4.81EE146 pKa = 4.06DD147 pKa = 5.81LIDD150 pKa = 4.03EE151 pKa = 4.93DD152 pKa = 5.03EE153 pKa = 4.38YY154 pKa = 12.04

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559KIZ5|A0A559KIZ5_9MOLU Glycerol-3-phosphate acyltransferase OS=Candidatus Phytoplasma pini OX=267362 GN=plsY PE=3 SV=1
EEE2 pKa = 3.8TKKK5 pKa = 10.79YY6 pKa = 10.63RR7 pKa = 11.84GKKK10 pKa = 9.96YY11 pKa = 9.28GEEE14 pKa = 3.8MKKK17 pKa = 11.05KKK19 pKa = 10.73IRR21 pKa = 11.84FHHH24 pKa = 6.54DD25 pKa = 4.08VANRR29 pKa = 11.84MNYYY33 pKa = 8.88KK34 pKa = 10.18NAVYYY39 pKa = 9.73PTLPLKKK46 pKa = 10.81NGKKK50 pKa = 8.1VSFIPKKK57 pKa = 8.86RR58 pKa = 11.84KKK60 pKa = 9.06SEEE63 pKa = 4.48DD64 pKa = 3.7YY65 pKa = 11.43NGITMTLRR73 pKa = 11.84SDDD76 pKa = 3.69APCKKK81 pKa = 10.37RR82 pKa = 11.84SQRR85 pKa = 11.84TQKKK89 pKa = 10.58NNSRR93 pKa = 11.84VLLLN

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

404

0

404

123395

50

1471

305.4

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.311 ± 0.099

0.908 ± 0.029

4.777 ± 0.079

6.484 ± 0.116

6.781 ± 0.147

4.159 ± 0.118

1.693 ± 0.041

11.556 ± 0.125

11.831 ± 0.143

10.561 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.036

7.663 ± 0.124

2.453 ± 0.049

4.107 ± 0.083

2.806 ± 0.081

6.214 ± 0.084

4.019 ± 0.078

4.374 ± 0.089

0.489 ± 0.031

4.132 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski