Weissella ceti
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1338 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075TZF1|A0A075TZF1_9LACO Addiction module antitoxin RelB/DinJ family OS=Weissella ceti OX=759620 GN=WS74_0743 PE=3 SV=1
MM1 pKa = 7.61 ASFGEE6 pKa = 4.19 RR7 pKa = 11.84 MLNEE11 pKa = 4.04 LALGQVDD18 pKa = 3.81 EE19 pKa = 4.83 AKK21 pKa = 10.62 KK22 pKa = 10.8 SFASALRR29 pKa = 11.84 HH30 pKa = 6.77 DD31 pKa = 5.55 DD32 pKa = 4.03 DD33 pKa = 3.97 DD34 pKa = 4.66 TIYY37 pKa = 11.32 SLAEE41 pKa = 3.53 EE42 pKa = 5.18 LYY44 pKa = 10.96 ALGMSNQAKK53 pKa = 9.72 RR54 pKa = 11.84 AYY56 pKa = 9.08 EE57 pKa = 3.99 KK58 pKa = 10.94 LLEE61 pKa = 4.57 RR62 pKa = 11.84 YY63 pKa = 9.11 PDD65 pKa = 3.54 EE66 pKa = 4.75 DD67 pKa = 3.47 QLRR70 pKa = 11.84 TALADD75 pKa = 3.37 IAIDD79 pKa = 4.73 EE80 pKa = 5.33 DD81 pKa = 3.98 DD82 pKa = 3.62 TDD84 pKa = 3.73 AAMGYY89 pKa = 10.0 LADD92 pKa = 5.07 IKK94 pKa = 9.69 PTSNAYY100 pKa = 10.36 LEE102 pKa = 4.43 SLLVLADD109 pKa = 4.59 LYY111 pKa = 11.1 QSEE114 pKa = 4.37 GLYY117 pKa = 10.54 EE118 pKa = 3.99 PAEE121 pKa = 4.28 AKK123 pKa = 10.62 LLEE126 pKa = 4.2 AHH128 pKa = 6.41 NLAPEE133 pKa = 3.89 EE134 pKa = 3.97 AVIGFALAEE143 pKa = 4.26 YY144 pKa = 9.84 YY145 pKa = 10.11 FASAKK150 pKa = 8.85 YY151 pKa = 9.72 QEE153 pKa = 5.11 AIPYY157 pKa = 9.28 YY158 pKa = 10.39 RR159 pKa = 11.84 EE160 pKa = 3.78 LLKK163 pKa = 11.24 AGDD166 pKa = 4.28 RR167 pKa = 11.84 YY168 pKa = 10.73 FSGTDD173 pKa = 3.02 VASRR177 pKa = 11.84 IGVAYY182 pKa = 10.28 ALVGDD187 pKa = 4.06 VTNALAYY194 pKa = 10.49 LEE196 pKa = 4.28 QIKK199 pKa = 7.65 TTEE202 pKa = 3.96 LTPDD206 pKa = 2.88 VRR208 pKa = 11.84 FQLGMLYY215 pKa = 10.44 AADD218 pKa = 4.2 EE219 pKa = 4.33 EE220 pKa = 4.84 THH222 pKa = 6.12 QKK224 pKa = 10.91 AIDD227 pKa = 3.65 TFEE230 pKa = 3.96 EE231 pKa = 4.21 LMEE234 pKa = 5.96 IDD236 pKa = 4.01 ASYY239 pKa = 9.04 ATLYY243 pKa = 10.52 EE244 pKa = 4.07 PLGNLYY250 pKa = 10.17 EE251 pKa = 4.52 HH252 pKa = 7.03 AQQDD256 pKa = 3.58 EE257 pKa = 4.49 DD258 pKa = 4.73 ALRR261 pKa = 11.84 TYY263 pKa = 10.47 QAGLAVDD270 pKa = 3.5 QFNTKK275 pKa = 9.83 LVEE278 pKa = 3.89 RR279 pKa = 11.84 AAILSEE285 pKa = 3.87 RR286 pKa = 11.84 LGSSEE291 pKa = 4.76 QAEE294 pKa = 4.11 QLYY297 pKa = 10.47 KK298 pKa = 10.8 EE299 pKa = 4.43 GLRR302 pKa = 11.84 NNPGDD307 pKa = 3.61 PTLVLDD313 pKa = 3.88 YY314 pKa = 11.25 SDD316 pKa = 3.78 WLVLQNKK323 pKa = 8.26 YY324 pKa = 10.98 AEE326 pKa = 4.85 NIALLNDD333 pKa = 3.44 YY334 pKa = 10.58 LADD337 pKa = 4.64 DD338 pKa = 4.69 EE339 pKa = 6.19 ADD341 pKa = 3.42 IDD343 pKa = 3.94 PVIYY347 pKa = 10.48 RR348 pKa = 11.84 NLAQSYY354 pKa = 7.64 TALEE358 pKa = 4.7 DD359 pKa = 3.67 YY360 pKa = 11.3 EE361 pKa = 4.61 MATQYY366 pKa = 9.18 WQAAVPLFIDD376 pKa = 3.81 DD377 pKa = 4.52 ANFLRR382 pKa = 11.84 EE383 pKa = 3.6 AFFYY387 pKa = 10.66 FRR389 pKa = 11.84 EE390 pKa = 4.23 NGNQEE395 pKa = 4.1 LALEE399 pKa = 4.19 TLTRR403 pKa = 11.84 YY404 pKa = 9.94 VEE406 pKa = 4.21 LVPTDD411 pKa = 4.2 LEE413 pKa = 4.18 MSEE416 pKa = 4.29 TLAALADD423 pKa = 3.52 TDD425 pKa = 4.16 YY426 pKa = 11.97
Molecular weight: 48.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.24
Thurlkill 3.821
EMBOSS 3.859
Sillero 4.101
Patrickios 1.481
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A075TXN0|A0A075TXN0_9LACO Uncharacterized protein OS=Weissella ceti OX=759620 GN=WS74_0148 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1338
0
1338
398033
29
1673
297.5
33.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.893 ± 0.095
0.2 ± 0.012
6.208 ± 0.066
6.564 ± 0.096
4.129 ± 0.049
6.926 ± 0.069
1.858 ± 0.033
6.616 ± 0.061
5.487 ± 0.094
9.504 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.232 ± 0.038
4.463 ± 0.045
3.703 ± 0.062
4.314 ± 0.062
4.219 ± 0.049
5.232 ± 0.054
6.38 ± 0.054
7.759 ± 0.052
1.079 ± 0.026
3.232 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here