Luteitalea pratensis
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6189 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143PP57|A0A143PP57_9BACT Uncharacterized protein OS=Luteitalea pratensis OX=1855912 GN=LuPra_03710 PE=4 SV=1
MM1 pKa = 7.36 LCVYY5 pKa = 10.13 CRR7 pKa = 11.84 QQPVDD12 pKa = 3.88 PRR14 pKa = 11.84 WQPFCSEE21 pKa = 3.94 RR22 pKa = 11.84 CQWLDD27 pKa = 3.28 LSHH30 pKa = 7.03 WLDD33 pKa = 2.65 GDD35 pKa = 3.91 YY36 pKa = 10.6 RR37 pKa = 11.84 VEE39 pKa = 4.61 GPADD43 pKa = 3.81 DD44 pKa = 5.07 TPPDD48 pKa = 4.06 MDD50 pKa = 5.49 PDD52 pKa = 4.18 DD53 pKa = 5.44 PEE55 pKa = 4.39 ATDD58 pKa = 3.59 LL59 pKa = 4.62
Molecular weight: 6.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.503
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.745
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A143PPU9|A0A143PPU9_9BACT Uroporphyrinogen decarboxylase OS=Luteitalea pratensis OX=1855912 GN=hemE PE=3 SV=1
MM1 pKa = 7.36 SRR3 pKa = 11.84 PLVVLVLGVTALMALSWAVGRR24 pKa = 11.84 VIFWLRR30 pKa = 11.84 ATFPPNRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 GRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 3.03
Molecular weight: 4.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6189
0
6189
2193066
29
2794
354.3
38.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.818 ± 0.044
0.765 ± 0.01
5.686 ± 0.02
4.978 ± 0.023
3.392 ± 0.019
8.644 ± 0.027
2.19 ± 0.014
3.984 ± 0.02
2.193 ± 0.023
10.034 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.052 ± 0.013
2.243 ± 0.025
5.775 ± 0.021
3.275 ± 0.018
8.229 ± 0.032
5.394 ± 0.023
6.061 ± 0.025
8.496 ± 0.025
1.543 ± 0.013
2.248 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here