Granulosicoccus antarcticus IMCC3135
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6680 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z2NMS6|A0A2Z2NMS6_9GAMM Fe2OG dioxygenase domain-containing protein OS=Granulosicoccus antarcticus IMCC3135 OX=1192854 GN=IMCC3135_12175 PE=3 SV=1
MM1 pKa = 7.49 WRR3 pKa = 11.84 RR4 pKa = 11.84 ASVFAVHH11 pKa = 6.76 IAILGSLTACSEE23 pKa = 4.58 SPFSGSEE30 pKa = 3.92 TADD33 pKa = 3.25 SDD35 pKa = 4.22 TQTSSATPSSPTSQTNDD52 pKa = 2.94 GSDD55 pKa = 3.79 SLSQPQSVVVAQANPTVVEE74 pKa = 4.32 IPDD77 pKa = 3.4 VAAATPEE84 pKa = 3.83 PTPIVVNPFTPVPQPTSEE102 pKa = 4.04 PDD104 pKa = 3.14 EE105 pKa = 4.4 EE106 pKa = 4.64 QEE108 pKa = 5.03 SEE110 pKa = 4.3 PDD112 pKa = 3.52 DD113 pKa = 4.87 SPDD116 pKa = 3.32 EE117 pKa = 4.19 VVANSDD123 pKa = 3.41 SDD125 pKa = 3.81 TDD127 pKa = 3.93 GNTGSDD133 pKa = 3.12 AGADD137 pKa = 3.76 SEE139 pKa = 5.71 AEE141 pKa = 4.51 LDD143 pKa = 3.84 TADD146 pKa = 4.24 NSPEE150 pKa = 4.02 STEE153 pKa = 4.07 TPEE156 pKa = 3.92 TGVADD161 pKa = 4.09 SQSSPTDD168 pKa = 3.26 NGDD171 pKa = 3.12 TDD173 pKa = 4.16 LAEE176 pKa = 4.31 TDD178 pKa = 3.8 VDD180 pKa = 4.46 DD181 pKa = 5.02 SDD183 pKa = 4.71 SAATDD188 pKa = 3.84 TEE190 pKa = 4.33 ASTDD194 pKa = 4.08 DD195 pKa = 3.62 NTQDD199 pKa = 5.16 AEE201 pKa = 4.52 TTDD204 pKa = 4.15 DD205 pKa = 4.08 SAPEE209 pKa = 3.67 TDD211 pKa = 3.49 VAEE214 pKa = 4.46 EE215 pKa = 4.34 SSDD218 pKa = 3.47 DD219 pKa = 3.72 TAEE222 pKa = 3.9 QDD224 pKa = 3.61 STDD227 pKa = 4.17 SIVAIDD233 pKa = 4.1 PADD236 pKa = 3.69 SGEE239 pKa = 4.31 TEE241 pKa = 3.9 QSKK244 pKa = 11.4 AEE246 pKa = 3.99 AEE248 pKa = 4.48 NNFSSHH254 pKa = 4.34 TTAGFIGKK262 pKa = 9.03 IVDD265 pKa = 4.47 DD266 pKa = 4.9 SIQVSWEE273 pKa = 4.0 VEE275 pKa = 4.14 PGALGYY281 pKa = 10.22 NVYY284 pKa = 10.98 LEE286 pKa = 4.39 GKK288 pKa = 9.58 YY289 pKa = 7.97 VTTVWEE295 pKa = 3.95 NSYY298 pKa = 11.27 VEE300 pKa = 4.07 TDD302 pKa = 4.47 LYY304 pKa = 10.37 DD305 pKa = 3.18 QNYY308 pKa = 8.93 YY309 pKa = 10.88 YY310 pKa = 10.18 EE311 pKa = 4.28 IQSFDD316 pKa = 3.74 EE317 pKa = 4.18 TKK319 pKa = 10.49 KK320 pKa = 10.28 IYY322 pKa = 9.42 WYY324 pKa = 10.32 NATGLTVKK332 pKa = 9.9 VRR334 pKa = 11.84 SAGRR338 pKa = 11.84 VDD340 pKa = 3.61 SSRR343 pKa = 11.84 ATAKK347 pKa = 10.72 ADD349 pKa = 4.12 LLNDD353 pKa = 3.47 YY354 pKa = 10.51 QLIFSDD360 pKa = 4.22 EE361 pKa = 4.41 FTGSTLDD368 pKa = 3.38 TSKK371 pKa = 10.87 WNTALLWGDD380 pKa = 3.38 QIIINGEE387 pKa = 3.79 VQFYY391 pKa = 11.35 VDD393 pKa = 4.61 INNKK397 pKa = 9.28 PDD399 pKa = 3.37 FGFNPFTFDD408 pKa = 5.02 GEE410 pKa = 4.42 HH411 pKa = 5.86 LTINSIKK418 pKa = 9.65 TPSEE422 pKa = 3.74 LSEE425 pKa = 4.12 KK426 pKa = 10.97 AKK428 pKa = 10.1 GQPYY432 pKa = 10.16 LSGVITSYY440 pKa = 11.34 DD441 pKa = 3.5 ALQFIYY447 pKa = 10.05 GYY449 pKa = 10.99 AEE451 pKa = 3.7 TRR453 pKa = 11.84 AKK455 pKa = 9.38 VTFGRR460 pKa = 11.84 GYY462 pKa = 10.02 WPAFWLLNAYY472 pKa = 9.19 YY473 pKa = 10.14 GQGGDD478 pKa = 4.33 DD479 pKa = 3.81 PEE481 pKa = 5.43 IDD483 pKa = 2.86 IMEE486 pKa = 5.4 FIGHH490 pKa = 6.37 DD491 pKa = 3.33 QDD493 pKa = 4.46 VVYY496 pKa = 8.4 HH497 pKa = 6.4 TYY499 pKa = 10.3 HH500 pKa = 6.79 YY501 pKa = 10.63 YY502 pKa = 10.68 DD503 pKa = 3.49 EE504 pKa = 4.86 NGEE507 pKa = 4.06 LRR509 pKa = 11.84 STKK512 pKa = 9.89 SQPTPGVDD520 pKa = 3.39 YY521 pKa = 11.56 SEE523 pKa = 5.45 DD524 pKa = 3.1 FHH526 pKa = 7.47 TFGVEE531 pKa = 3.92 WKK533 pKa = 9.11 PGTIIYY539 pKa = 10.0 YY540 pKa = 8.9 VDD542 pKa = 4.32 GIEE545 pKa = 3.92 VHH547 pKa = 6.52 RR548 pKa = 11.84 VVDD551 pKa = 4.12 PKK553 pKa = 11.14 VSQQTMYY560 pKa = 11.01 VIANTAMGGWWAGDD574 pKa = 3.65 PDD576 pKa = 4.3 EE577 pKa = 4.63 TTPFPGEE584 pKa = 3.69 FMIDD588 pKa = 3.63 YY589 pKa = 10.03 IRR591 pKa = 11.84 VYY593 pKa = 10.74 QRR595 pKa = 11.84 TTPYY599 pKa = 11.32 DD600 pKa = 3.5 DD601 pKa = 4.7 VLFNDD606 pKa = 5.03 DD607 pKa = 3.18 MTGIPFADD615 pKa = 5.44 EE616 pKa = 4.46 IPGSAIPNHH625 pKa = 6.77 RR626 pKa = 11.84 PTPAQWPAGYY636 pKa = 8.83 PHH638 pKa = 6.97 AQQ640 pKa = 3.11
Molecular weight: 69.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.503
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.859
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A2Z2NWN7|A0A2Z2NWN7_9GAMM 8-oxo-dGTP diphosphatase OS=Granulosicoccus antarcticus IMCC3135 OX=1192854 GN=mutT PE=3 SV=1
MM1 pKa = 7.53 INSIPPQGLVLLSIVAIQVGAAVAIHH27 pKa = 6.57 LFPVLGASGTVAVRR41 pKa = 11.84 IIFSAILLGLAARR54 pKa = 11.84 TGLRR58 pKa = 11.84 TLVQTFRR65 pKa = 11.84 LHH67 pKa = 5.41 WKK69 pKa = 9.4 LLVVFGLSIAAMNLFFYY86 pKa = 10.42 QALARR91 pKa = 11.84 IPLGAAVAFEE101 pKa = 4.69 FIGPLGVAALASRR114 pKa = 11.84 RR115 pKa = 11.84 LTQFAWIALAALGIVLLSPLSGADD139 pKa = 3.77 LDD141 pKa = 4.8 PIGILFALMAGTGWACFIIFAGRR164 pKa = 11.84 VGKK167 pKa = 9.91 LIPGNDD173 pKa = 3.4 GLAIGMAVAALAMIPFALPVAGILVTNPLILLASIGVALLSTTIPFTFEE222 pKa = 3.19 FTALKK227 pKa = 10.11 RR228 pKa = 11.84 LPARR232 pKa = 11.84 TYY234 pKa = 9.98 GVLVSLEE241 pKa = 4.23 PGVAALVGALLLGEE255 pKa = 4.82 RR256 pKa = 11.84 IGLQGMIAITCVIIAAIGITLSDD279 pKa = 3.64 ARR281 pKa = 11.84 TPPP284 pKa = 3.23
Molecular weight: 29.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.531
IPC_protein 10.496
Toseland 10.599
ProMoST 10.379
Dawson 10.701
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.774
Grimsley 10.76
Solomon 10.862
Lehninger 10.818
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.643
Patrickios 10.57
IPC_peptide 10.862
IPC2_peptide 9.736
IPC2.peptide.svr19 8.564
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6680
0
6680
2309472
29
8704
345.7
37.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.024 ± 0.032
1.035 ± 0.013
6.086 ± 0.077
5.862 ± 0.028
3.704 ± 0.02
7.601 ± 0.031
2.137 ± 0.018
5.678 ± 0.025
3.376 ± 0.033
10.503 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.528 ± 0.022
3.47 ± 0.031
4.434 ± 0.024
4.013 ± 0.026
5.665 ± 0.044
7.147 ± 0.038
5.656 ± 0.054
7.271 ± 0.027
1.304 ± 0.014
2.507 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here