Mycobacterium phage Marley1013

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pipefishvirus; unclassified Pipefishvirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FGG4|A0A3G8FGG4_9CAUD Uncharacterized protein OS=Mycobacterium phage Marley1013 OX=2488787 GN=34 PE=4 SV=1
MM1 pKa = 7.18AQDD4 pKa = 3.55VNLGAVFDD12 pKa = 4.8DD13 pKa = 5.21ALTADD18 pKa = 3.87EE19 pKa = 4.77WWDD22 pKa = 3.52EE23 pKa = 4.23FNANACCPDD32 pKa = 3.74SVTAAARR39 pKa = 11.84LCGCGGSGRR48 pKa = 11.84IPTGISRR55 pKa = 11.84LLL57 pKa = 3.6

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FJC1|A0A3G8FJC1_9CAUD Uncharacterized protein OS=Mycobacterium phage Marley1013 OX=2488787 GN=80 PE=4 SV=1
MM1 pKa = 7.56APKK4 pKa = 8.8KK5 pKa = 9.17TPATRR10 pKa = 11.84EE11 pKa = 3.97SPVFIRR17 pKa = 11.84MASPEE22 pKa = 3.86DD23 pKa = 3.23RR24 pKa = 11.84TRR26 pKa = 11.84FKK28 pKa = 10.96VAAAEE33 pKa = 4.72DD34 pKa = 4.03GLSYY38 pKa = 11.53ADD40 pKa = 5.46LISSLLDD47 pKa = 3.4LRR49 pKa = 11.84DD50 pKa = 3.3DD51 pKa = 3.78RR52 pKa = 11.84NRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84AAMAHH62 pKa = 6.2PLDD65 pKa = 3.6TRR67 pKa = 11.84RR68 pKa = 11.84NAGG71 pKa = 3.17

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

21987

36

1912

217.7

23.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.411 ± 0.3

1.105 ± 0.159

6.922 ± 0.268

5.954 ± 0.281

2.638 ± 0.105

9.401 ± 0.661

1.728 ± 0.133

4.853 ± 0.197

3.247 ± 0.182

7.827 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.123

2.824 ± 0.137

6.017 ± 0.228

3.129 ± 0.15

6.731 ± 0.433

5.453 ± 0.191

6.681 ± 0.23

7.732 ± 0.255

1.947 ± 0.149

2.247 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski