Amino acid dipepetide frequency for Mycobacterium phage Marley1013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.238AlaAla: 16.238 ± 1.124
0.864AlaCys: 0.864 ± 0.191
7.96AlaAsp: 7.96 ± 0.53
7.914AlaGlu: 7.914 ± 0.729
2.32AlaPhe: 2.32 ± 0.334
9.324AlaGly: 9.324 ± 1.095
1.728AlaHis: 1.728 ± 0.326
5.185AlaIle: 5.185 ± 0.46
3.457AlaLys: 3.457 ± 0.373
10.234AlaLeu: 10.234 ± 0.808
2.638AlaMet: 2.638 ± 0.353
3.32AlaAsn: 3.32 ± 0.413
6.049AlaPro: 6.049 ± 0.599
2.774AlaGln: 2.774 ± 0.41
7.641AlaArg: 7.641 ± 0.687
5.231AlaSer: 5.231 ± 0.467
6.55AlaThr: 6.55 ± 0.593
9.642AlaVal: 9.642 ± 0.531
2.547AlaTrp: 2.547 ± 0.28
2.502AlaTyr: 2.502 ± 0.362
0.0AlaXaa: 0.0 ± 0.0
Cys
0.955CysAla: 0.955 ± 0.227
0.091CysCys: 0.091 ± 0.07
0.728CysAsp: 0.728 ± 0.162
0.682CysGlu: 0.682 ± 0.259
0.182CysPhe: 0.182 ± 0.109
1.546CysGly: 1.546 ± 0.365
0.409CysHis: 0.409 ± 0.139
0.318CysIle: 0.318 ± 0.108
0.227CysLys: 0.227 ± 0.09
0.546CysLeu: 0.546 ± 0.198
0.182CysMet: 0.182 ± 0.085
0.318CysAsn: 0.318 ± 0.104
1.41CysPro: 1.41 ± 0.299
0.182CysGln: 0.182 ± 0.085
1.046CysArg: 1.046 ± 0.236
0.773CysSer: 0.773 ± 0.205
0.318CysThr: 0.318 ± 0.131
0.637CysVal: 0.637 ± 0.184
0.318CysTrp: 0.318 ± 0.155
0.182CysTyr: 0.182 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
7.869AspAla: 7.869 ± 0.482
0.546AspCys: 0.546 ± 0.145
5.867AspAsp: 5.867 ± 0.689
5.549AspGlu: 5.549 ± 0.519
1.865AspPhe: 1.865 ± 0.257
6.595AspGly: 6.595 ± 0.567
1.455AspHis: 1.455 ± 0.311
2.82AspIle: 2.82 ± 0.292
2.092AspLys: 2.092 ± 0.248
5.64AspLeu: 5.64 ± 0.5
1.365AspMet: 1.365 ± 0.25
1.956AspAsn: 1.956 ± 0.265
4.639AspPro: 4.639 ± 0.492
2.411AspGln: 2.411 ± 0.329
4.639AspArg: 4.639 ± 0.56
3.411AspSer: 3.411 ± 0.47
3.957AspThr: 3.957 ± 0.343
4.503AspVal: 4.503 ± 0.492
1.046AspTrp: 1.046 ± 0.184
1.001AspTyr: 1.001 ± 0.202
0.0AspXaa: 0.0 ± 0.0
Glu
6.55GluAla: 6.55 ± 0.6
0.91GluCys: 0.91 ± 0.229
3.912GluAsp: 3.912 ± 0.535
2.229GluGlu: 2.229 ± 0.378
2.092GluPhe: 2.092 ± 0.266
4.23GluGly: 4.23 ± 0.41
1.546GluHis: 1.546 ± 0.258
3.821GluIle: 3.821 ± 0.368
2.411GluLys: 2.411 ± 0.277
4.048GluLeu: 4.048 ± 0.415
1.637GluMet: 1.637 ± 0.292
1.501GluAsn: 1.501 ± 0.2
3.821GluPro: 3.821 ± 0.44
2.456GluGln: 2.456 ± 0.328
4.321GluArg: 4.321 ± 0.492
3.184GluSer: 3.184 ± 0.405
3.684GluThr: 3.684 ± 0.528
4.412GluVal: 4.412 ± 0.447
1.365GluTrp: 1.365 ± 0.223
1.41GluTyr: 1.41 ± 0.275
0.0GluXaa: 0.0 ± 0.0
Phe
2.865PheAla: 2.865 ± 0.312
0.182PheCys: 0.182 ± 0.077
2.82PheAsp: 2.82 ± 0.393
1.41PheGlu: 1.41 ± 0.251
0.5PhePhe: 0.5 ± 0.127
2.774PheGly: 2.774 ± 0.418
0.455PheHis: 0.455 ± 0.185
0.773PheIle: 0.773 ± 0.194
0.773PheLys: 0.773 ± 0.181
2.001PheLeu: 2.001 ± 0.233
0.318PheMet: 0.318 ± 0.118
0.728PheAsn: 0.728 ± 0.156
1.501PhePro: 1.501 ± 0.238
0.728PheGln: 0.728 ± 0.177
1.774PheArg: 1.774 ± 0.327
1.365PheSer: 1.365 ± 0.199
1.819PheThr: 1.819 ± 0.322
2.411PheVal: 2.411 ± 0.373
0.637PheTrp: 0.637 ± 0.173
0.546PheTyr: 0.546 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
7.732GlyAla: 7.732 ± 1.249
0.955GlyCys: 0.955 ± 0.251
5.185GlyAsp: 5.185 ± 0.465
4.912GlyGlu: 4.912 ± 0.443
2.547GlyPhe: 2.547 ± 0.321
12.872GlyGly: 12.872 ± 2.661
1.455GlyHis: 1.455 ± 0.244
4.958GlyIle: 4.958 ± 0.53
3.957GlyLys: 3.957 ± 0.456
7.823GlyLeu: 7.823 ± 0.699
2.32GlyMet: 2.32 ± 0.415
3.002GlyAsn: 3.002 ± 0.39
3.775GlyPro: 3.775 ± 0.427
2.956GlyGln: 2.956 ± 0.452
5.64GlyArg: 5.64 ± 0.591
5.685GlySer: 5.685 ± 0.722
6.777GlyThr: 6.777 ± 0.7
7.505GlyVal: 7.505 ± 0.664
2.001GlyTrp: 2.001 ± 0.296
1.956GlyTyr: 1.956 ± 0.325
0.0GlyXaa: 0.0 ± 0.0
His
1.774HisAla: 1.774 ± 0.304
0.273HisCys: 0.273 ± 0.108
1.501HisAsp: 1.501 ± 0.264
0.682HisGlu: 0.682 ± 0.227
0.591HisPhe: 0.591 ± 0.164
1.592HisGly: 1.592 ± 0.276
0.455HisHis: 0.455 ± 0.164
0.546HisIle: 0.546 ± 0.166
0.364HisLys: 0.364 ± 0.12
1.546HisLeu: 1.546 ± 0.265
0.364HisMet: 0.364 ± 0.144
0.364HisAsn: 0.364 ± 0.136
1.137HisPro: 1.137 ± 0.287
0.546HisGln: 0.546 ± 0.183
1.592HisArg: 1.592 ± 0.231
0.819HisSer: 0.819 ± 0.19
1.137HisThr: 1.137 ± 0.251
1.046HisVal: 1.046 ± 0.234
0.455HisTrp: 0.455 ± 0.151
0.5HisTyr: 0.5 ± 0.141
0.0HisXaa: 0.0 ± 0.0
Ile
6.049IleAla: 6.049 ± 0.415
0.455IleCys: 0.455 ± 0.172
4.048IleAsp: 4.048 ± 0.358
3.912IleGlu: 3.912 ± 0.452
1.046IlePhe: 1.046 ± 0.214
3.593IleGly: 3.593 ± 0.47
0.864IleHis: 0.864 ± 0.173
2.001IleIle: 2.001 ± 0.352
1.546IleLys: 1.546 ± 0.327
3.047IleLeu: 3.047 ± 0.342
0.773IleMet: 0.773 ± 0.166
1.592IleAsn: 1.592 ± 0.264
2.638IlePro: 2.638 ± 0.274
1.819IleGln: 1.819 ± 0.286
3.684IleArg: 3.684 ± 0.376
2.684IleSer: 2.684 ± 0.379
3.502IleThr: 3.502 ± 0.452
4.048IleVal: 4.048 ± 0.486
0.591IleTrp: 0.591 ± 0.164
0.637IleTyr: 0.637 ± 0.159
0.0IleXaa: 0.0 ± 0.0
Lys
4.094LysAla: 4.094 ± 0.5
0.273LysCys: 0.273 ± 0.101
1.819LysAsp: 1.819 ± 0.282
1.319LysGlu: 1.319 ± 0.201
1.228LysPhe: 1.228 ± 0.244
2.411LysGly: 2.411 ± 0.31
0.546LysHis: 0.546 ± 0.155
2.001LysIle: 2.001 ± 0.347
0.864LysLys: 0.864 ± 0.216
3.138LysLeu: 3.138 ± 0.378
0.546LysMet: 0.546 ± 0.172
1.365LysAsn: 1.365 ± 0.23
1.637LysPro: 1.637 ± 0.254
0.864LysGln: 0.864 ± 0.256
2.32LysArg: 2.32 ± 0.396
1.956LysSer: 1.956 ± 0.295
1.41LysThr: 1.41 ± 0.334
3.093LysVal: 3.093 ± 0.415
0.546LysTrp: 0.546 ± 0.133
1.046LysTyr: 1.046 ± 0.196
0.0LysXaa: 0.0 ± 0.0
Leu
9.006LeuAla: 9.006 ± 0.643
0.955LeuCys: 0.955 ± 0.221
5.594LeuAsp: 5.594 ± 0.469
3.275LeuGlu: 3.275 ± 0.403
2.047LeuPhe: 2.047 ± 0.322
7.96LeuGly: 7.96 ± 0.719
1.546LeuHis: 1.546 ± 0.298
4.048LeuIle: 4.048 ± 0.401
2.547LeuLys: 2.547 ± 0.432
5.64LeuLeu: 5.64 ± 0.552
1.501LeuMet: 1.501 ± 0.236
1.865LeuAsn: 1.865 ± 0.308
4.776LeuPro: 4.776 ± 0.856
1.865LeuGln: 1.865 ± 0.3
5.731LeuArg: 5.731 ± 0.563
4.457LeuSer: 4.457 ± 0.568
6.732LeuThr: 6.732 ± 0.691
5.14LeuVal: 5.14 ± 0.531
1.274LeuTrp: 1.274 ± 0.265
2.32LeuTyr: 2.32 ± 0.311
0.0LeuXaa: 0.0 ± 0.0
Met
3.275MetAla: 3.275 ± 0.432
0.182MetCys: 0.182 ± 0.103
0.955MetAsp: 0.955 ± 0.2
0.91MetGlu: 0.91 ± 0.161
0.5MetPhe: 0.5 ± 0.168
1.41MetGly: 1.41 ± 0.262
0.273MetHis: 0.273 ± 0.106
1.228MetIle: 1.228 ± 0.224
0.773MetLys: 0.773 ± 0.173
1.683MetLeu: 1.683 ± 0.304
0.455MetMet: 0.455 ± 0.139
0.682MetAsn: 0.682 ± 0.159
1.819MetPro: 1.819 ± 0.292
0.682MetGln: 0.682 ± 0.169
0.728MetArg: 0.728 ± 0.204
1.365MetSer: 1.365 ± 0.238
2.638MetThr: 2.638 ± 0.369
1.319MetVal: 1.319 ± 0.26
0.318MetTrp: 0.318 ± 0.14
0.318MetTyr: 0.318 ± 0.108
0.0MetXaa: 0.0 ± 0.0
Asn
3.866AsnAla: 3.866 ± 0.458
0.273AsnCys: 0.273 ± 0.103
1.683AsnAsp: 1.683 ± 0.256
1.183AsnGlu: 1.183 ± 0.222
0.591AsnPhe: 0.591 ± 0.148
3.093AsnGly: 3.093 ± 0.419
0.546AsnHis: 0.546 ± 0.176
1.137AsnIle: 1.137 ± 0.309
0.864AsnLys: 0.864 ± 0.159
2.183AsnLeu: 2.183 ± 0.404
0.409AsnMet: 0.409 ± 0.145
0.546AsnAsn: 0.546 ± 0.16
3.047AsnPro: 3.047 ± 0.397
0.364AsnGln: 0.364 ± 0.121
1.546AsnArg: 1.546 ± 0.244
1.001AsnSer: 1.001 ± 0.226
2.138AsnThr: 2.138 ± 0.378
2.274AsnVal: 2.274 ± 0.266
0.91AsnTrp: 0.91 ± 0.193
0.591AsnTyr: 0.591 ± 0.145
0.0AsnXaa: 0.0 ± 0.0
Pro
6.186ProAla: 6.186 ± 0.565
0.682ProCys: 0.682 ± 0.222
5.367ProAsp: 5.367 ± 0.661
6.14ProGlu: 6.14 ± 0.6
1.637ProPhe: 1.637 ± 0.278
5.685ProGly: 5.685 ± 0.479
0.591ProHis: 0.591 ± 0.126
3.138ProIle: 3.138 ± 0.382
1.501ProLys: 1.501 ± 0.232
3.275ProLeu: 3.275 ± 0.287
1.319ProMet: 1.319 ± 0.255
1.365ProAsn: 1.365 ± 0.325
4.275ProPro: 4.275 ± 0.534
1.228ProGln: 1.228 ± 0.179
2.911ProArg: 2.911 ± 0.354
3.548ProSer: 3.548 ± 0.466
3.821ProThr: 3.821 ± 0.376
5.322ProVal: 5.322 ± 0.454
1.228ProTrp: 1.228 ± 0.291
0.91ProTyr: 0.91 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
4.275GlnAla: 4.275 ± 0.409
0.455GlnCys: 0.455 ± 0.155
1.183GlnAsp: 1.183 ± 0.22
1.592GlnGlu: 1.592 ± 0.326
1.183GlnPhe: 1.183 ± 0.223
2.593GlnGly: 2.593 ± 0.321
0.546GlnHis: 0.546 ± 0.151
1.91GlnIle: 1.91 ± 0.296
0.819GlnLys: 0.819 ± 0.178
2.365GlnLeu: 2.365 ± 0.333
1.137GlnMet: 1.137 ± 0.233
0.546GlnAsn: 0.546 ± 0.142
1.683GlnPro: 1.683 ± 0.253
1.956GlnGln: 1.956 ± 0.302
2.32GlnArg: 2.32 ± 0.303
1.365GlnSer: 1.365 ± 0.241
1.774GlnThr: 1.774 ± 0.27
1.956GlnVal: 1.956 ± 0.295
0.5GlnTrp: 0.5 ± 0.148
1.137GlnTyr: 1.137 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
6.55ArgAla: 6.55 ± 0.564
1.001ArgCys: 1.001 ± 0.236
4.776ArgAsp: 4.776 ± 0.448
3.912ArgGlu: 3.912 ± 0.53
1.91ArgPhe: 1.91 ± 0.292
4.685ArgGly: 4.685 ± 0.484
1.228ArgHis: 1.228 ± 0.239
3.093ArgIle: 3.093 ± 0.376
1.865ArgLys: 1.865 ± 0.329
5.276ArgLeu: 5.276 ± 0.493
1.865ArgMet: 1.865 ± 0.297
2.092ArgAsn: 2.092 ± 0.298
3.639ArgPro: 3.639 ± 0.465
2.593ArgGln: 2.593 ± 0.339
6.186ArgArg: 6.186 ± 0.903
3.457ArgSer: 3.457 ± 0.394
4.003ArgThr: 4.003 ± 0.426
5.231ArgVal: 5.231 ± 0.498
1.774ArgTrp: 1.774 ± 0.378
2.183ArgTyr: 2.183 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
5.958SerAla: 5.958 ± 0.501
0.455SerCys: 0.455 ± 0.148
3.184SerAsp: 3.184 ± 0.378
2.774SerGlu: 2.774 ± 0.334
1.501SerPhe: 1.501 ± 0.264
6.459SerGly: 6.459 ± 0.661
0.91SerHis: 0.91 ± 0.15
2.729SerIle: 2.729 ± 0.364
2.229SerLys: 2.229 ± 0.394
4.184SerLeu: 4.184 ± 0.481
1.046SerMet: 1.046 ± 0.228
1.546SerAsn: 1.546 ± 0.325
3.639SerPro: 3.639 ± 0.347
2.092SerGln: 2.092 ± 0.312
3.684SerArg: 3.684 ± 0.41
3.002SerSer: 3.002 ± 0.36
3.639SerThr: 3.639 ± 0.447
3.32SerVal: 3.32 ± 0.395
1.092SerTrp: 1.092 ± 0.223
1.092SerTyr: 1.092 ± 0.275
0.0SerXaa: 0.0 ± 0.0
Thr
8.051ThrAla: 8.051 ± 0.636
0.864ThrCys: 0.864 ± 0.236
4.048ThrAsp: 4.048 ± 0.377
3.593ThrGlu: 3.593 ± 0.389
1.865ThrPhe: 1.865 ± 0.222
6.686ThrGly: 6.686 ± 0.649
0.637ThrHis: 0.637 ± 0.153
3.821ThrIle: 3.821 ± 0.443
2.638ThrLys: 2.638 ± 0.308
5.367ThrLeu: 5.367 ± 0.464
1.41ThrMet: 1.41 ± 0.268
1.501ThrAsn: 1.501 ± 0.27
4.457ThrPro: 4.457 ± 0.433
1.865ThrGln: 1.865 ± 0.315
4.412ThrArg: 4.412 ± 0.554
3.32ThrSer: 3.32 ± 0.457
4.139ThrThr: 4.139 ± 0.504
5.731ThrVal: 5.731 ± 0.472
1.137ThrTrp: 1.137 ± 0.218
1.274ThrTyr: 1.274 ± 0.275
0.0ThrXaa: 0.0 ± 0.0
Val
8.824ValAla: 8.824 ± 0.677
0.728ValCys: 0.728 ± 0.198
5.594ValAsp: 5.594 ± 0.641
5.049ValGlu: 5.049 ± 0.448
1.546ValPhe: 1.546 ± 0.266
7.186ValGly: 7.186 ± 0.525
1.319ValHis: 1.319 ± 0.273
3.184ValIle: 3.184 ± 0.385
2.411ValLys: 2.411 ± 0.307
6.186ValLeu: 6.186 ± 0.461
1.41ValMet: 1.41 ± 0.284
2.911ValAsn: 2.911 ± 0.35
4.23ValPro: 4.23 ± 0.4
2.274ValGln: 2.274 ± 0.25
3.866ValArg: 3.866 ± 0.549
5.413ValSer: 5.413 ± 0.641
5.549ValThr: 5.549 ± 0.649
6.368ValVal: 6.368 ± 0.53
1.274ValTrp: 1.274 ± 0.225
2.001ValTyr: 2.001 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.683TrpAla: 1.683 ± 0.287
0.546TrpCys: 0.546 ± 0.176
1.501TrpAsp: 1.501 ± 0.295
1.41TrpGlu: 1.41 ± 0.231
0.546TrpPhe: 0.546 ± 0.187
1.092TrpGly: 1.092 ± 0.191
0.409TrpHis: 0.409 ± 0.117
0.864TrpIle: 0.864 ± 0.179
0.728TrpLys: 0.728 ± 0.196
1.592TrpLeu: 1.592 ± 0.274
0.364TrpMet: 0.364 ± 0.136
0.455TrpAsn: 0.455 ± 0.143
1.137TrpPro: 1.137 ± 0.246
0.546TrpGln: 0.546 ± 0.14
1.546TrpArg: 1.546 ± 0.263
1.546TrpSer: 1.546 ± 0.246
1.728TrpThr: 1.728 ± 0.341
1.092TrpVal: 1.092 ± 0.27
0.227TrpTrp: 0.227 ± 0.105
0.455TrpTyr: 0.455 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.32TyrAla: 2.32 ± 0.307
0.364TyrCys: 0.364 ± 0.133
1.501TyrAsp: 1.501 ± 0.302
1.092TyrGlu: 1.092 ± 0.199
0.682TyrPhe: 0.682 ± 0.173
2.229TyrGly: 2.229 ± 0.298
0.364TyrHis: 0.364 ± 0.135
0.955TyrIle: 0.955 ± 0.21
0.409TyrLys: 0.409 ± 0.136
2.502TyrLeu: 2.502 ± 0.41
0.409TyrMet: 0.409 ± 0.128
0.591TyrAsn: 0.591 ± 0.179
0.864TyrPro: 0.864 ± 0.21
1.092TyrGln: 1.092 ± 0.23
1.728TyrArg: 1.728 ± 0.307
0.955TyrSer: 0.955 ± 0.258
1.501TyrThr: 1.501 ± 0.284
2.274TyrVal: 2.274 ± 0.376
0.227TyrTrp: 0.227 ± 0.108
0.409TyrTyr: 0.409 ± 0.148
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (21987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski