Amino acid dipepetide frequency for Microbacterium sp. TS-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.559AlaAla: 22.559 ± 0.249	0.664AlaCys: 0.664 ± 0.029	9.133AlaAsp: 9.133 ± 0.107	8.194AlaGlu: 8.194 ± 0.109	4.071AlaPhe: 4.071 ± 0.063	13.09AlaGly: 13.09 ± 0.142	2.426AlaHis: 2.426 ± 0.051	5.934AlaIle: 5.934 ± 0.079	2.185AlaLys: 2.185 ± 0.061	14.431AlaLeu: 14.431 ± 0.151	2.685AlaMet: 2.685 ± 0.055	2.101AlaAsn: 2.101 ± 0.046	7.076AlaPro: 7.076 ± 0.122	3.671AlaGln: 3.671 ± 0.06	10.096AlaArg: 10.096 ± 0.136	7.691AlaSer: 7.691 ± 0.09	7.74AlaThr: 7.74 ± 0.097	12.691AlaVal: 12.691 ± 0.139	2.041AlaTrp: 2.041 ± 0.046	2.364AlaTyr: 2.364 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.695CysAla: 0.695 ± 0.026	0.042CysCys: 0.042 ± 0.007	0.322CysAsp: 0.322 ± 0.018	0.252CysGlu: 0.252 ± 0.017	0.156CysPhe: 0.156 ± 0.013	0.509CysGly: 0.509 ± 0.022	0.116CysHis: 0.116 ± 0.011	0.168CysIle: 0.168 ± 0.013	0.04CysLys: 0.04 ± 0.006	0.398CysLeu: 0.398 ± 0.017	0.074CysMet: 0.074 ± 0.009	0.09CysAsn: 0.09 ± 0.009	0.244CysPro: 0.244 ± 0.016	0.104CysGln: 0.104 ± 0.011	0.334CysArg: 0.334 ± 0.02	0.282CysSer: 0.282 ± 0.017	0.302CysThr: 0.302 ± 0.019	0.382CysVal: 0.382 ± 0.017	0.056CysTrp: 0.056 ± 0.008	0.09CysTyr: 0.09 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.022AspAla: 10.022 ± 0.124	0.215AspCys: 0.215 ± 0.014	4.986AspAsp: 4.986 ± 0.086	4.672AspGlu: 4.672 ± 0.075	1.701AspPhe: 1.701 ± 0.039	6.367AspGly: 6.367 ± 0.094	1.204AspHis: 1.204 ± 0.036	2.61AspIle: 2.61 ± 0.056	0.826AspLys: 0.826 ± 0.032	6.412AspLeu: 6.412 ± 0.092	0.791AspMet: 0.791 ± 0.029	0.909AspAsn: 0.909 ± 0.03	4.265AspPro: 4.265 ± 0.066	1.428AspGln: 1.428 ± 0.036	4.836AspArg: 4.836 ± 0.076	2.479AspSer: 2.479 ± 0.047	2.95AspThr: 2.95 ± 0.06	5.842AspVal: 5.842 ± 0.085	0.939AspTrp: 0.939 ± 0.032	1.252AspTyr: 1.252 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.131GluAla: 7.131 ± 0.096	0.222GluCys: 0.222 ± 0.015	2.5GluAsp: 2.5 ± 0.055	2.524GluGlu: 2.524 ± 0.054	1.63GluPhe: 1.63 ± 0.041	3.826GluGly: 3.826 ± 0.072	1.357GluHis: 1.357 ± 0.04	2.839GluIle: 2.839 ± 0.062	1.238GluLys: 1.238 ± 0.038	6.419GluLeu: 6.419 ± 0.095	0.889GluMet: 0.889 ± 0.032	1.259GluAsn: 1.259 ± 0.036	3.173GluPro: 3.173 ± 0.069	2.046GluGln: 2.046 ± 0.047	5.579GluArg: 5.579 ± 0.096	2.806GluSer: 2.806 ± 0.059	3.169GluThr: 3.169 ± 0.058	4.565GluVal: 4.565 ± 0.065	0.842GluTrp: 0.842 ± 0.031	1.095GluTyr: 1.095 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.583PheAla: 4.583 ± 0.074	0.14PheCys: 0.14 ± 0.011	2.395PheAsp: 2.395 ± 0.056	1.655PheGlu: 1.655 ± 0.039	1.095PhePhe: 1.095 ± 0.04	3.3PheGly: 3.3 ± 0.061	0.54PheHis: 0.54 ± 0.021	1.224PheIle: 1.224 ± 0.038	0.356PheLys: 0.356 ± 0.019	2.841PheLeu: 2.841 ± 0.06	0.423PheMet: 0.423 ± 0.021	0.599PheAsn: 0.599 ± 0.026	1.376PhePro: 1.376 ± 0.038	0.733PheGln: 0.733 ± 0.026	1.816PheArg: 1.816 ± 0.042	1.775PheSer: 1.775 ± 0.041	2.06PheThr: 2.06 ± 0.046	2.963PheVal: 2.963 ± 0.053	0.506PheTrp: 0.506 ± 0.021	0.668PheTyr: 0.668 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	11.543GlyAla: 11.543 ± 0.12	0.562GlyCys: 0.562 ± 0.026	5.559GlyAsp: 5.559 ± 0.091	5.108GlyGlu: 5.108 ± 0.076	3.252GlyPhe: 3.252 ± 0.06	7.799GlyGly: 7.799 ± 0.098	1.779GlyHis: 1.779 ± 0.046	4.838GlyIle: 4.838 ± 0.079	1.801GlyLys: 1.801 ± 0.055	8.669GlyLeu: 8.669 ± 0.104	1.956GlyMet: 1.956 ± 0.049	1.596GlyAsn: 1.596 ± 0.046	3.92GlyPro: 3.92 ± 0.071	2.307GlyGln: 2.307 ± 0.048	6.672GlyArg: 6.672 ± 0.076	5.489GlySer: 5.489 ± 0.082	5.33GlyThr: 5.33 ± 0.079	8.068GlyVal: 8.068 ± 0.093	1.638GlyTrp: 1.638 ± 0.043	2.14GlyTyr: 2.14 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.35HisAla: 2.35 ± 0.054	0.101HisCys: 0.101 ± 0.009	1.41HisAsp: 1.41 ± 0.038	1.086HisGlu: 1.086 ± 0.039	0.498HisPhe: 0.498 ± 0.022	1.881HisGly: 1.881 ± 0.045	0.533HisHis: 0.533 ± 0.025	0.686HisIle: 0.686 ± 0.026	0.243HisLys: 0.243 ± 0.014	1.909HisLeu: 1.909 ± 0.049	0.28HisMet: 0.28 ± 0.019	0.296HisAsn: 0.296 ± 0.018	1.524HisPro: 1.524 ± 0.042	0.418HisGln: 0.418 ± 0.021	1.658HisArg: 1.658 ± 0.047	0.86HisSer: 0.86 ± 0.034	1.007HisThr: 1.007 ± 0.031	1.588HisVal: 1.588 ± 0.041	0.26HisTrp: 0.26 ± 0.015	0.376HisTyr: 0.376 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.264IleAla: 7.264 ± 0.089	0.214IleCys: 0.214 ± 0.014	3.664IleAsp: 3.664 ± 0.061	2.796IleGlu: 2.796 ± 0.06	1.132IlePhe: 1.132 ± 0.037	4.53IleGly: 4.53 ± 0.078	0.678IleHis: 0.678 ± 0.026	1.767IleIle: 1.767 ± 0.053	0.607IleLys: 0.607 ± 0.029	3.655IleLeu: 3.655 ± 0.068	0.574IleMet: 0.574 ± 0.028	0.785IleAsn: 0.785 ± 0.031	2.492IlePro: 2.492 ± 0.051	0.892IleGln: 0.892 ± 0.03	2.805IleArg: 2.805 ± 0.055	2.281IleSer: 2.281 ± 0.043	2.677IleThr: 2.677 ± 0.058	4.869IleVal: 4.869 ± 0.077	0.502IleTrp: 0.502 ± 0.02	0.722IleTyr: 0.722 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	1.881LysAla: 1.881 ± 0.057	0.05LysCys: 0.05 ± 0.007	0.912LysAsp: 0.912 ± 0.033	0.721LysGlu: 0.721 ± 0.031	0.395LysPhe: 0.395 ± 0.019	1.261LysGly: 1.261 ± 0.044	0.349LysHis: 0.349 ± 0.019	0.723LysIle: 0.723 ± 0.03	0.617LysLys: 0.617 ± 0.034	1.578LysLeu: 1.578 ± 0.045	0.287LysMet: 0.287 ± 0.018	0.426LysAsn: 0.426 ± 0.024	1.005LysPro: 1.005 ± 0.033	0.578LysGln: 0.578 ± 0.023	1.331LysArg: 1.331 ± 0.04	0.942LysSer: 0.942 ± 0.039	1.106LysThr: 1.106 ± 0.043	1.364LysVal: 1.364 ± 0.045	0.197LysTrp: 0.197 ± 0.013	0.367LysTyr: 0.367 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.865LeuAla: 14.865 ± 0.135	0.478LeuCys: 0.478 ± 0.022	6.735LeuAsp: 6.735 ± 0.089	4.933LeuGlu: 4.933 ± 0.08	2.903LeuPhe: 2.903 ± 0.067	9.051LeuGly: 9.051 ± 0.114	1.805LeuHis: 1.805 ± 0.046	4.313LeuIle: 4.313 ± 0.082	1.371LeuLys: 1.371 ± 0.048	9.833LeuLeu: 9.833 ± 0.148	1.578LeuMet: 1.578 ± 0.044	1.6LeuAsn: 1.6 ± 0.043	5.576LeuPro: 5.576 ± 0.072	2.373LeuGln: 2.373 ± 0.046	7.877LeuArg: 7.877 ± 0.093	5.796LeuSer: 5.796 ± 0.062	6.108LeuThr: 6.108 ± 0.085	9.412LeuVal: 9.412 ± 0.131	1.365LeuTrp: 1.365 ± 0.038	1.637LeuTyr: 1.637 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.005MetAla: 2.005 ± 0.05	0.083MetCys: 0.083 ± 0.01	0.71MetAsp: 0.71 ± 0.025	0.565MetGlu: 0.565 ± 0.024	0.513MetPhe: 0.513 ± 0.022	1.273MetGly: 1.273 ± 0.04	0.305MetHis: 0.305 ± 0.018	0.818MetIle: 0.818 ± 0.027	0.358MetLys: 0.358 ± 0.018	1.928MetLeu: 1.928 ± 0.047	0.304MetMet: 0.304 ± 0.018	0.429MetAsn: 0.429 ± 0.02	1.153MetPro: 1.153 ± 0.034	0.527MetGln: 0.527 ± 0.022	1.42MetArg: 1.42 ± 0.034	1.475MetSer: 1.475 ± 0.042	1.756MetThr: 1.756 ± 0.044	1.23MetVal: 1.23 ± 0.034	0.223MetTrp: 0.223 ± 0.015	0.288MetTyr: 0.288 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.321AsnAla: 2.321 ± 0.05	0.092AsnCys: 0.092 ± 0.01	1.167AsnAsp: 1.167 ± 0.038	0.899AsnGlu: 0.899 ± 0.03	0.584AsnPhe: 0.584 ± 0.025	1.748AsnGly: 1.748 ± 0.052	0.343AsnHis: 0.343 ± 0.019	0.793AsnIle: 0.793 ± 0.029	0.341AsnLys: 0.341 ± 0.022	1.869AsnLeu: 1.869 ± 0.045	0.291AsnMet: 0.291 ± 0.018	0.41AsnAsn: 0.41 ± 0.019	1.413AsnPro: 1.413 ± 0.04	0.488AsnGln: 0.488 ± 0.025	1.216AsnArg: 1.216 ± 0.034	0.843AsnSer: 0.843 ± 0.028	1.013AsnThr: 1.013 ± 0.037	1.582AsnVal: 1.582 ± 0.038	0.264AsnTrp: 0.264 ± 0.017	0.422AsnTyr: 0.422 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.962ProAla: 7.962 ± 0.117	0.148ProCys: 0.148 ± 0.012	4.091ProAsp: 4.091 ± 0.067	3.849ProGlu: 3.849 ± 0.067	1.749ProPhe: 1.749 ± 0.038	5.294ProGly: 5.294 ± 0.082	1.129ProHis: 1.129 ± 0.033	2.093ProIle: 2.093 ± 0.043	0.779ProLys: 0.779 ± 0.031	5.016ProLeu: 5.016 ± 0.085	0.873ProMet: 0.873 ± 0.026	0.927ProAsn: 0.927 ± 0.032	2.401ProPro: 2.401 ± 0.068	1.492ProGln: 1.492 ± 0.03	3.798ProArg: 3.798 ± 0.062	3.096ProSer: 3.096 ± 0.056	3.347ProThr: 3.347 ± 0.07	4.995ProVal: 4.995 ± 0.078	0.861ProTrp: 0.861 ± 0.028	1.023ProTyr: 1.023 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.201GlnAla: 3.201 ± 0.056	0.093GlnCys: 0.093 ± 0.01	1.147GlnAsp: 1.147 ± 0.036	1.159GlnGlu: 1.159 ± 0.033	0.854GlnPhe: 0.854 ± 0.029	1.859GlnGly: 1.859 ± 0.042	0.57GlnHis: 0.57 ± 0.022	1.302GlnIle: 1.302 ± 0.039	0.544GlnLys: 0.544 ± 0.022	2.987GlnLeu: 2.987 ± 0.056	0.422GlnMet: 0.422 ± 0.022	0.613GlnAsn: 0.613 ± 0.024	1.505GlnPro: 1.505 ± 0.042	1.044GlnGln: 1.044 ± 0.034	2.522GlnArg: 2.522 ± 0.053	1.393GlnSer: 1.393 ± 0.038	1.524GlnThr: 1.524 ± 0.038	2.144GlnVal: 2.144 ± 0.045	0.444GlnTrp: 0.444 ± 0.02	0.56GlnTyr: 0.56 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	9.729ArgAla: 9.729 ± 0.127	0.313ArgCys: 0.313 ± 0.017	4.692ArgAsp: 4.692 ± 0.072	4.553ArgGlu: 4.553 ± 0.073	2.442ArgPhe: 2.442 ± 0.043	6.104ArgGly: 6.104 ± 0.079	1.619ArgHis: 1.619 ± 0.041	3.76ArgIle: 3.76 ± 0.06	1.153ArgLys: 1.153 ± 0.039	7.528ArgLeu: 7.528 ± 0.099	1.854ArgMet: 1.854 ± 0.044	1.35ArgAsn: 1.35 ± 0.037	4.046ArgPro: 4.046 ± 0.07	1.933ArgGln: 1.933 ± 0.04	7.427ArgArg: 7.427 ± 0.115	4.455ArgSer: 4.455 ± 0.066	4.463ArgThr: 4.463 ± 0.062	6.578ArgVal: 6.578 ± 0.076	1.275ArgTrp: 1.275 ± 0.039	1.569ArgTyr: 1.569 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.465SerAla: 7.465 ± 0.092	0.276SerCys: 0.276 ± 0.018	3.391SerAsp: 3.391 ± 0.057	2.659SerGlu: 2.659 ± 0.055	1.946SerPhe: 1.946 ± 0.041	5.999SerGly: 5.999 ± 0.077	0.965SerHis: 0.965 ± 0.029	2.538SerIle: 2.538 ± 0.056	0.803SerLys: 0.803 ± 0.031	5.337SerLeu: 5.337 ± 0.066	1.139SerMet: 1.139 ± 0.035	1.016SerAsn: 1.016 ± 0.035	3.314SerPro: 3.314 ± 0.059	1.326SerGln: 1.326 ± 0.039	3.982SerArg: 3.982 ± 0.061	3.368SerSer: 3.368 ± 0.062	3.297SerThr: 3.297 ± 0.054	4.748SerVal: 4.748 ± 0.071	1.017SerTrp: 1.017 ± 0.031	1.104SerTyr: 1.104 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	8.267ThrAla: 8.267 ± 0.117	0.248ThrCys: 0.248 ± 0.015	3.665ThrAsp: 3.665 ± 0.063	2.849ThrGlu: 2.849 ± 0.047	1.874ThrPhe: 1.874 ± 0.046	5.723ThrGly: 5.723 ± 0.085	1.064ThrHis: 1.064 ± 0.031	2.746ThrIle: 2.746 ± 0.054	0.956ThrLys: 0.956 ± 0.037	5.738ThrLeu: 5.738 ± 0.082	0.937ThrMet: 0.937 ± 0.029	1.112ThrAsn: 1.112 ± 0.036	3.829ThrPro: 3.829 ± 0.075	1.395ThrGln: 1.395 ± 0.036	3.829ThrArg: 3.829 ± 0.06	3.363ThrSer: 3.363 ± 0.056	3.801ThrThr: 3.801 ± 0.075	5.944ThrVal: 5.944 ± 0.08	0.895ThrTrp: 0.895 ± 0.027	1.202ThrTyr: 1.202 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	12.889ValAla: 12.889 ± 0.135	0.498ValCys: 0.498 ± 0.022	6.039ValAsp: 6.039 ± 0.076	4.861ValGlu: 4.861 ± 0.077	3.016ValPhe: 3.016 ± 0.054	7.393ValGly: 7.393 ± 0.087	1.561ValHis: 1.561 ± 0.043	4.342ValIle: 4.342 ± 0.069	1.357ValLys: 1.357 ± 0.042	9.296ValLeu: 9.296 ± 0.113	1.441ValMet: 1.441 ± 0.04	1.669ValAsn: 1.669 ± 0.046	4.812ValPro: 4.812 ± 0.071	2.071ValGln: 2.071 ± 0.042	6.583ValArg: 6.583 ± 0.097	5.351ValSer: 5.351 ± 0.076	5.769ValThr: 5.769 ± 0.079	9.663ValVal: 9.663 ± 0.129	1.216ValTrp: 1.216 ± 0.039	1.553ValTyr: 1.553 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.57TrpAla: 1.57 ± 0.04	0.114TrpCys: 0.114 ± 0.009	0.856TrpAsp: 0.856 ± 0.027	0.698TrpGlu: 0.698 ± 0.025	0.563TrpPhe: 0.563 ± 0.026	1.136TrpGly: 1.136 ± 0.033	0.355TrpHis: 0.355 ± 0.017	0.71TrpIle: 0.71 ± 0.023	0.253TrpLys: 0.253 ± 0.016	1.732TrpLeu: 1.732 ± 0.042	0.328TrpMet: 0.328 ± 0.018	0.451TrpAsn: 0.451 ± 0.025	0.738TrpPro: 0.738 ± 0.028	0.561TrpGln: 0.561 ± 0.022	1.392TrpArg: 1.392 ± 0.04	0.946TrpSer: 0.946 ± 0.035	0.937TrpThr: 0.937 ± 0.032	1.225TrpVal: 1.225 ± 0.035	0.365TrpTrp: 0.365 ± 0.02	0.294TrpTyr: 0.294 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.544TyrAla: 2.544 ± 0.054	0.102TyrCys: 0.102 ± 0.01	1.332TyrAsp: 1.332 ± 0.037	1.074TyrGlu: 1.074 ± 0.032	0.677TyrPhe: 0.677 ± 0.027	1.884TyrGly: 1.884 ± 0.04	0.269TyrHis: 0.269 ± 0.018	0.699TyrIle: 0.699 ± 0.027	0.26TyrLys: 0.26 ± 0.018	2.029TyrLeu: 2.029 ± 0.04	0.235TyrMet: 0.235 ± 0.017	0.464TyrAsn: 0.464 ± 0.021	1.058TyrPro: 1.058 ± 0.035	0.502TyrGln: 0.502 ± 0.022	1.671TyrArg: 1.671 ± 0.042	1.0TyrSer: 1.0 ± 0.035	1.083TyrThr: 1.083 ± 0.036	1.527TyrVal: 1.527 ± 0.032	0.316TyrTrp: 0.316 ± 0.019	0.469TyrTyr: 0.469 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3155 proteins (1046449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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