Clostridiales bacterium KA00274

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133Y7L2|A0A133Y7L2_9FIRM Putative proton-coupled thiamine transporter YuaJ OS=Clostridiales bacterium KA00274 OX=1497955 GN=HMPREF1872_01209 PE=4 SV=1
MM1 pKa = 7.12KK2 pKa = 10.57VSVAYY7 pKa = 10.64YY8 pKa = 10.38SEE10 pKa = 4.15TGNTQEE16 pKa = 4.82LADD19 pKa = 4.38MLTAALQTEE28 pKa = 4.4GAEE31 pKa = 4.35VYY33 pKa = 7.55EE34 pKa = 4.45TAVSDD39 pKa = 3.74VDD41 pKa = 3.68SSEE44 pKa = 4.75FLDD47 pKa = 4.02SDD49 pKa = 4.22LLVFATPATGTEE61 pKa = 4.25EE62 pKa = 3.72IDD64 pKa = 3.75QIEE67 pKa = 4.31MQAFIDD73 pKa = 5.2DD74 pKa = 3.85IASQLSGRR82 pKa = 11.84KK83 pKa = 8.98VFLCGTFGWGDD94 pKa = 3.86GEE96 pKa = 5.65YY97 pKa = 9.35MQAWQEE103 pKa = 3.79AMADD107 pKa = 4.05LGCQFAHH114 pKa = 6.95EE115 pKa = 4.83PFTCLEE121 pKa = 4.23SPDD124 pKa = 5.27DD125 pKa = 3.79EE126 pKa = 7.1AEE128 pKa = 4.35DD129 pKa = 3.78KK130 pKa = 10.98LAEE133 pKa = 3.96IAKK136 pKa = 9.91EE137 pKa = 3.92LFRR140 pKa = 11.84SII142 pKa = 4.58

Molecular weight: 15.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133Y7S0|A0A133Y7S0_9FIRM Cell division protein FtsZ OS=Clostridiales bacterium KA00274 OX=1497955 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.6AVPKK5 pKa = 10.43RR6 pKa = 11.84RR7 pKa = 11.84WSKK10 pKa = 9.16EE11 pKa = 2.98RR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84SNWKK21 pKa = 9.66LVAPALVEE29 pKa = 4.06CTSCHH34 pKa = 6.21EE35 pKa = 4.08LRR37 pKa = 11.84QAHH40 pKa = 6.4RR41 pKa = 11.84PCKK44 pKa = 9.78ACGVYY49 pKa = 10.14RR50 pKa = 11.84GRR52 pKa = 11.84QVIDD56 pKa = 3.25VAKK59 pKa = 10.94AEE61 pKa = 4.13

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1471

0

1471

464541

31

1684

315.8

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.552 ± 0.055

1.373 ± 0.023

5.228 ± 0.055

6.37 ± 0.053

4.328 ± 0.049

5.605 ± 0.059

1.781 ± 0.027

6.72 ± 0.057

7.36 ± 0.056

11.198 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.03

5.07 ± 0.043

3.303 ± 0.032

4.18 ± 0.048

3.951 ± 0.048

6.212 ± 0.043

5.157 ± 0.039

6.313 ± 0.052

0.923 ± 0.021

3.948 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski