Amino acid dipepetide frequency for Clostridiales bacterium KA00274

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.643AlaAla: 6.643 ± 0.153
1.673AlaCys: 1.673 ± 0.069
4.859AlaAsp: 4.859 ± 0.098
5.931AlaGlu: 5.931 ± 0.131
3.66AlaPhe: 3.66 ± 0.092
4.753AlaGly: 4.753 ± 0.107
1.298AlaHis: 1.298 ± 0.051
5.933AlaIle: 5.933 ± 0.128
8.019AlaLys: 8.019 ± 0.151
9.153AlaLeu: 9.153 ± 0.172
2.269AlaMet: 2.269 ± 0.089
5.102AlaAsn: 5.102 ± 0.123
2.056AlaPro: 2.056 ± 0.066
2.566AlaGln: 2.566 ± 0.076
2.844AlaArg: 2.844 ± 0.078
5.25AlaSer: 5.25 ± 0.119
3.954AlaThr: 3.954 ± 0.096
5.026AlaVal: 5.026 ± 0.11
0.852AlaTrp: 0.852 ± 0.041
3.683AlaTyr: 3.683 ± 0.083
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.046
0.215CysCys: 0.215 ± 0.022
0.611CysAsp: 0.611 ± 0.032
0.717CysGlu: 0.717 ± 0.041
0.741CysPhe: 0.741 ± 0.039
0.936CysGly: 0.936 ± 0.049
0.366CysHis: 0.366 ± 0.033
0.818CysIle: 0.818 ± 0.04
0.971CysLys: 0.971 ± 0.051
2.049CysLeu: 2.049 ± 0.077
0.327CysMet: 0.327 ± 0.023
0.519CysAsn: 0.519 ± 0.034
0.502CysPro: 0.502 ± 0.04
0.76CysGln: 0.76 ± 0.042
0.536CysArg: 0.536 ± 0.032
0.827CysSer: 0.827 ± 0.045
0.549CysThr: 0.549 ± 0.036
0.769CysVal: 0.769 ± 0.043
0.108CysTrp: 0.108 ± 0.016
0.484CysTyr: 0.484 ± 0.035
0.0CysXaa: 0.0 ± 0.0
Asp
3.685AspAla: 3.685 ± 0.113
0.771AspCys: 0.771 ± 0.044
2.603AspAsp: 2.603 ± 0.087
3.84AspGlu: 3.84 ± 0.095
2.956AspPhe: 2.956 ± 0.081
3.13AspGly: 3.13 ± 0.095
0.678AspHis: 0.678 ± 0.036
3.668AspIle: 3.668 ± 0.099
4.275AspLys: 4.275 ± 0.091
6.264AspLeu: 6.264 ± 0.125
1.339AspMet: 1.339 ± 0.063
2.454AspAsn: 2.454 ± 0.081
1.634AspPro: 1.634 ± 0.062
1.49AspGln: 1.49 ± 0.06
1.845AspArg: 1.845 ± 0.08
2.837AspSer: 2.837 ± 0.086
2.443AspThr: 2.443 ± 0.073
3.149AspVal: 3.149 ± 0.088
0.605AspTrp: 0.605 ± 0.045
2.609AspTyr: 2.609 ± 0.081
0.0AspXaa: 0.0 ± 0.0
Glu
5.935GluAla: 5.935 ± 0.123
0.603GluCys: 0.603 ± 0.036
2.68GluAsp: 2.68 ± 0.088
3.929GluGlu: 3.929 ± 0.122
2.495GluPhe: 2.495 ± 0.077
2.633GluGly: 2.633 ± 0.079
1.436GluHis: 1.436 ± 0.056
4.813GluIle: 4.813 ± 0.113
4.938GluLys: 4.938 ± 0.11
7.741GluLeu: 7.741 ± 0.159
1.666GluMet: 1.666 ± 0.057
3.406GluAsn: 3.406 ± 0.092
1.985GluPro: 1.985 ± 0.073
3.548GluGln: 3.548 ± 0.112
3.195GluArg: 3.195 ± 0.1
2.979GluSer: 2.979 ± 0.088
3.281GluThr: 3.281 ± 0.094
3.92GluVal: 3.92 ± 0.102
0.489GluTrp: 0.489 ± 0.033
2.032GluTyr: 2.032 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
3.827PheAla: 3.827 ± 0.093
0.598PheCys: 0.598 ± 0.036
2.275PheAsp: 2.275 ± 0.072
1.998PheGlu: 1.998 ± 0.064
1.993PhePhe: 1.993 ± 0.073
2.532PheGly: 2.532 ± 0.074
0.624PheHis: 0.624 ± 0.038
2.975PheIle: 2.975 ± 0.104
2.872PheLys: 2.872 ± 0.091
4.15PheLeu: 4.15 ± 0.103
1.184PheMet: 1.184 ± 0.05
2.504PheAsn: 2.504 ± 0.072
1.391PhePro: 1.391 ± 0.058
1.453PheGln: 1.453 ± 0.052
1.515PheArg: 1.515 ± 0.055
3.498PheSer: 3.498 ± 0.093
2.504PheThr: 2.504 ± 0.077
3.04PheVal: 3.04 ± 0.085
0.474PheTrp: 0.474 ± 0.026
1.873PheTyr: 1.873 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
4.028GlyAla: 4.028 ± 0.1
0.758GlyCys: 0.758 ± 0.04
2.656GlyAsp: 2.656 ± 0.081
3.675GlyGlu: 3.675 ± 0.09
2.499GlyPhe: 2.499 ± 0.083
3.541GlyGly: 3.541 ± 0.112
1.132GlyHis: 1.132 ± 0.057
4.264GlyIle: 4.264 ± 0.111
4.46GlyLys: 4.46 ± 0.109
5.95GlyLeu: 5.95 ± 0.119
1.5GlyMet: 1.5 ± 0.062
2.478GlyAsn: 2.478 ± 0.089
1.268GlyPro: 1.268 ± 0.053
2.424GlyGln: 2.424 ± 0.081
2.308GlyArg: 2.308 ± 0.079
3.406GlySer: 3.406 ± 0.082
3.143GlyThr: 3.143 ± 0.093
3.83GlyVal: 3.83 ± 0.113
0.428GlyTrp: 0.428 ± 0.032
2.303GlyTyr: 2.303 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
1.328HisAla: 1.328 ± 0.054
0.278HisCys: 0.278 ± 0.023
1.035HisAsp: 1.035 ± 0.051
1.023HisGlu: 1.023 ± 0.042
0.898HisPhe: 0.898 ± 0.041
1.171HisGly: 1.171 ± 0.061
0.355HisHis: 0.355 ± 0.029
1.156HisIle: 1.156 ± 0.054
1.281HisLys: 1.281 ± 0.055
1.952HisLeu: 1.952 ± 0.071
0.459HisMet: 0.459 ± 0.033
0.923HisAsn: 0.923 ± 0.05
0.697HisPro: 0.697 ± 0.042
0.603HisGln: 0.603 ± 0.038
0.637HisArg: 0.637 ± 0.04
1.074HisSer: 1.074 ± 0.049
0.829HisThr: 0.829 ± 0.042
1.087HisVal: 1.087 ± 0.048
0.153HisTrp: 0.153 ± 0.021
0.872HisTyr: 0.872 ± 0.05
0.0HisXaa: 0.0 ± 0.0
Ile
5.728IleAla: 5.728 ± 0.113
1.251IleCys: 1.251 ± 0.052
3.873IleAsp: 3.873 ± 0.109
3.799IleGlu: 3.799 ± 0.105
2.964IlePhe: 2.964 ± 0.091
4.03IleGly: 4.03 ± 0.119
0.939IleHis: 0.939 ± 0.054
4.422IleIle: 4.422 ± 0.136
4.891IleLys: 4.891 ± 0.093
6.716IleLeu: 6.716 ± 0.132
1.681IleMet: 1.681 ± 0.061
3.347IleAsn: 3.347 ± 0.084
2.467IlePro: 2.467 ± 0.069
2.015IleGln: 2.015 ± 0.076
2.562IleArg: 2.562 ± 0.083
5.061IleSer: 5.061 ± 0.126
3.375IleThr: 3.375 ± 0.081
4.682IleVal: 4.682 ± 0.119
0.663IleTrp: 0.663 ± 0.034
2.734IleTyr: 2.734 ± 0.088
0.0IleXaa: 0.0 ± 0.0
Lys
6.686LysAla: 6.686 ± 0.129
0.678LysCys: 0.678 ± 0.04
3.892LysAsp: 3.892 ± 0.102
5.326LysGlu: 5.326 ± 0.122
2.542LysPhe: 2.542 ± 0.074
3.272LysGly: 3.272 ± 0.09
1.46LysHis: 1.46 ± 0.054
4.581LysIle: 4.581 ± 0.098
4.613LysLys: 4.613 ± 0.124
8.893LysLeu: 8.893 ± 0.16
1.851LysMet: 1.851 ± 0.077
3.399LysAsn: 3.399 ± 0.085
2.364LysPro: 2.364 ± 0.07
4.71LysGln: 4.71 ± 0.106
3.496LysArg: 3.496 ± 0.085
4.125LysSer: 4.125 ± 0.11
3.799LysThr: 3.799 ± 0.105
4.714LysVal: 4.714 ± 0.109
0.646LysTrp: 0.646 ± 0.042
2.553LysTyr: 2.553 ± 0.068
0.0LysXaa: 0.0 ± 0.0
Leu
11.982LeuAla: 11.982 ± 0.215
1.5LeuCys: 1.5 ± 0.057
6.017LeuAsp: 6.017 ± 0.123
6.391LeuGlu: 6.391 ± 0.122
4.301LeuPhe: 4.301 ± 0.131
6.137LeuGly: 6.137 ± 0.135
2.075LeuHis: 2.075 ± 0.073
7.093LeuIle: 7.093 ± 0.147
7.511LeuLys: 7.511 ± 0.132
12.003LeuLeu: 12.003 ± 0.276
2.25LeuMet: 2.25 ± 0.077
5.638LeuAsn: 5.638 ± 0.122
4.732LeuPro: 4.732 ± 0.11
4.889LeuGln: 4.889 ± 0.124
4.419LeuArg: 4.419 ± 0.104
7.446LeuSer: 7.446 ± 0.135
6.48LeuThr: 6.48 ± 0.128
6.757LeuVal: 6.757 ± 0.125
0.872LeuTrp: 0.872 ± 0.048
3.487LeuTyr: 3.487 ± 0.102
0.0LeuXaa: 0.0 ± 0.0
Met
2.095MetAla: 2.095 ± 0.076
0.22MetCys: 0.22 ± 0.021
1.072MetAsp: 1.072 ± 0.044
1.317MetGlu: 1.317 ± 0.06
0.891MetPhe: 0.891 ± 0.047
1.33MetGly: 1.33 ± 0.056
0.564MetHis: 0.564 ± 0.034
1.541MetIle: 1.541 ± 0.059
1.599MetLys: 1.599 ± 0.049
2.732MetLeu: 2.732 ± 0.082
0.665MetMet: 0.665 ± 0.038
1.18MetAsn: 1.18 ± 0.048
1.178MetPro: 1.178 ± 0.054
1.619MetGln: 1.619 ± 0.06
1.162MetArg: 1.162 ± 0.056
1.5MetSer: 1.5 ± 0.056
1.391MetThr: 1.391 ± 0.06
1.324MetVal: 1.324 ± 0.064
0.196MetTrp: 0.196 ± 0.018
0.693MetTyr: 0.693 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.434AsnAla: 3.434 ± 0.084
0.822AsnCys: 0.822 ± 0.043
2.497AsnAsp: 2.497 ± 0.081
3.261AsnGlu: 3.261 ± 0.085
2.585AsnPhe: 2.585 ± 0.078
2.997AsnGly: 2.997 ± 0.095
0.73AsnHis: 0.73 ± 0.04
3.207AsnIle: 3.207 ± 0.074
3.522AsnLys: 3.522 ± 0.089
6.65AsnLeu: 6.65 ± 0.143
1.369AsnMet: 1.369 ± 0.055
2.482AsnAsn: 2.482 ± 0.079
1.795AsnPro: 1.795 ± 0.054
1.875AsnGln: 1.875 ± 0.077
1.856AsnArg: 1.856 ± 0.076
3.309AsnSer: 3.309 ± 0.106
2.129AsnThr: 2.129 ± 0.078
2.9AsnVal: 2.9 ± 0.082
0.678AsnTrp: 0.678 ± 0.042
2.6AsnTyr: 2.6 ± 0.073
0.0AsnXaa: 0.0 ± 0.0
Pro
2.596ProAla: 2.596 ± 0.072
0.349ProCys: 0.349 ± 0.032
1.985ProAsp: 1.985 ± 0.067
2.816ProGlu: 2.816 ± 0.075
1.569ProPhe: 1.569 ± 0.053
1.709ProGly: 1.709 ± 0.065
0.555ProHis: 0.555 ± 0.032
2.159ProIle: 2.159 ± 0.079
2.473ProLys: 2.473 ± 0.077
3.416ProLeu: 3.416 ± 0.088
0.693ProMet: 0.693 ± 0.037
2.095ProAsn: 2.095 ± 0.066
0.624ProPro: 0.624 ± 0.038
0.99ProGln: 0.99 ± 0.047
0.999ProArg: 0.999 ± 0.044
2.146ProSer: 2.146 ± 0.083
1.877ProThr: 1.877 ± 0.056
2.204ProVal: 2.204 ± 0.061
0.342ProTrp: 0.342 ± 0.027
1.427ProTyr: 1.427 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
6.034GlnAla: 6.034 ± 0.18
0.293GlnCys: 0.293 ± 0.027
1.683GlnAsp: 1.683 ± 0.065
2.243GlnGlu: 2.243 ± 0.08
1.455GlnPhe: 1.455 ± 0.057
2.034GlnGly: 2.034 ± 0.058
0.749GlnHis: 0.749 ± 0.04
3.167GlnIle: 3.167 ± 0.086
2.777GlnLys: 2.777 ± 0.079
4.174GlnLeu: 4.174 ± 0.118
0.954GlnMet: 0.954 ± 0.047
1.955GlnAsn: 1.955 ± 0.062
1.287GlnPro: 1.287 ± 0.057
1.828GlnGln: 1.828 ± 0.087
1.666GlnArg: 1.666 ± 0.064
2.295GlnSer: 2.295 ± 0.07
2.773GlnThr: 2.773 ± 0.095
2.921GlnVal: 2.921 ± 0.088
0.239GlnTrp: 0.239 ± 0.026
1.274GlnTyr: 1.274 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
2.695ArgAla: 2.695 ± 0.077
0.437ArgCys: 0.437 ± 0.03
1.888ArgAsp: 1.888 ± 0.075
2.876ArgGlu: 2.876 ± 0.1
1.709ArgPhe: 1.709 ± 0.063
2.006ArgGly: 2.006 ± 0.07
0.822ArgHis: 0.822 ± 0.041
2.65ArgIle: 2.65 ± 0.085
3.068ArgLys: 3.068 ± 0.09
4.589ArgLeu: 4.589 ± 0.102
1.059ArgMet: 1.059 ± 0.052
1.978ArgAsn: 1.978 ± 0.074
1.309ArgPro: 1.309 ± 0.059
2.129ArgGln: 2.129 ± 0.073
1.961ArgArg: 1.961 ± 0.081
2.183ArgSer: 2.183 ± 0.072
1.782ArgThr: 1.782 ± 0.067
2.241ArgVal: 2.241 ± 0.076
0.314ArgTrp: 0.314 ± 0.025
1.817ArgTyr: 1.817 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
4.568SerAla: 4.568 ± 0.103
0.784SerCys: 0.784 ± 0.046
3.386SerAsp: 3.386 ± 0.088
4.359SerGlu: 4.359 ± 0.119
2.96SerPhe: 2.96 ± 0.08
4.028SerGly: 4.028 ± 0.082
1.04SerHis: 1.04 ± 0.049
3.888SerIle: 3.888 ± 0.107
4.803SerLys: 4.803 ± 0.11
7.67SerLeu: 7.67 ± 0.16
1.348SerMet: 1.348 ± 0.057
3.113SerAsn: 3.113 ± 0.094
1.767SerPro: 1.767 ± 0.06
2.361SerGln: 2.361 ± 0.076
2.123SerArg: 2.123 ± 0.074
4.112SerSer: 4.112 ± 0.103
2.947SerThr: 2.947 ± 0.076
3.808SerVal: 3.808 ± 0.093
0.618SerTrp: 0.618 ± 0.037
2.443SerTyr: 2.443 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
4.439ThrAla: 4.439 ± 0.112
0.685ThrCys: 0.685 ± 0.041
2.96ThrAsp: 2.96 ± 0.084
3.66ThrGlu: 3.66 ± 0.101
2.25ThrPhe: 2.25 ± 0.071
3.285ThrGly: 3.285 ± 0.096
0.844ThrHis: 0.844 ± 0.042
3.53ThrIle: 3.53 ± 0.084
3.541ThrLys: 3.541 ± 0.104
5.474ThrLeu: 5.474 ± 0.126
1.096ThrMet: 1.096 ± 0.045
2.605ThrAsn: 2.605 ± 0.076
1.823ThrPro: 1.823 ± 0.063
1.559ThrGln: 1.559 ± 0.064
1.679ThrArg: 1.679 ± 0.061
3.182ThrSer: 3.182 ± 0.093
2.557ThrThr: 2.557 ± 0.09
3.769ThrVal: 3.769 ± 0.09
0.517ThrTrp: 0.517 ± 0.031
2.118ThrTyr: 2.118 ± 0.068
0.0ThrXaa: 0.0 ± 0.0
Val
5.044ValAla: 5.044 ± 0.109
1.059ValCys: 1.059 ± 0.051
3.774ValAsp: 3.774 ± 0.103
3.959ValGlu: 3.959 ± 0.102
2.594ValPhe: 2.594 ± 0.072
3.722ValGly: 3.722 ± 0.094
1.001ValHis: 1.001 ± 0.046
4.359ValIle: 4.359 ± 0.126
4.955ValLys: 4.955 ± 0.116
6.535ValLeu: 6.535 ± 0.125
1.494ValMet: 1.494 ± 0.06
3.3ValAsn: 3.3 ± 0.085
2.273ValPro: 2.273 ± 0.07
2.077ValGln: 2.077 ± 0.073
2.428ValArg: 2.428 ± 0.077
4.17ValSer: 4.17 ± 0.092
3.283ValThr: 3.283 ± 0.095
4.135ValVal: 4.135 ± 0.11
0.532ValTrp: 0.532 ± 0.033
2.439ValTyr: 2.439 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.59TrpAla: 0.59 ± 0.037
0.118TrpCys: 0.118 ± 0.016
0.366TrpAsp: 0.366 ± 0.03
0.411TrpGlu: 0.411 ± 0.03
0.338TrpPhe: 0.338 ± 0.027
0.478TrpGly: 0.478 ± 0.032
0.319TrpHis: 0.319 ± 0.027
0.441TrpIle: 0.441 ± 0.029
0.452TrpLys: 0.452 ± 0.028
1.156TrpLeu: 1.156 ± 0.053
0.211TrpMet: 0.211 ± 0.022
0.398TrpAsn: 0.398 ± 0.03
0.478TrpPro: 0.478 ± 0.035
1.171TrpGln: 1.171 ± 0.054
0.538TrpArg: 0.538 ± 0.037
0.549TrpSer: 0.549 ± 0.035
0.392TrpThr: 0.392 ± 0.031
0.385TrpVal: 0.385 ± 0.028
0.114TrpTrp: 0.114 ± 0.015
0.323TrpTyr: 0.323 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.261TyrAla: 3.261 ± 0.076
0.639TyrCys: 0.639 ± 0.039
2.163TyrAsp: 2.163 ± 0.087
2.172TyrGlu: 2.172 ± 0.078
1.879TyrPhe: 1.879 ± 0.067
2.327TyrGly: 2.327 ± 0.069
0.824TyrHis: 0.824 ± 0.043
2.534TyrIle: 2.534 ± 0.075
2.579TyrLys: 2.579 ± 0.081
4.712TyrLeu: 4.712 ± 0.106
0.852TyrMet: 0.852 ± 0.043
1.823TyrAsn: 1.823 ± 0.053
1.397TyrPro: 1.397 ± 0.057
1.735TyrGln: 1.735 ± 0.068
1.746TyrArg: 1.746 ± 0.064
2.176TyrSer: 2.176 ± 0.065
2.084TyrThr: 2.084 ± 0.061
2.471TyrVal: 2.471 ± 0.079
0.39TyrTrp: 0.39 ± 0.027
1.718TyrTyr: 1.718 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1471 proteins (464541 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski